Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
A peptide-hormone-inactivating endopeptidase in Xenopus laevis skin secretion.
Carvalho KM
,
Joudiou C
,
Boussetta H
,
Leseney AM
,
Cohen P
.
???displayArticle.abstract???
An endopeptidase was isolated from Xenopus laevis skin secretions. This enzyme, which has an apparent molecular mass of 100 kDa, performs a selective cleavage at the Xaa-Phe, Xaa-Leu, or Xaa-Ile bond (Xaa = Ser, Phe, Tyr, His, or Gly) of a number of peptide hormones, including atrial natriuretic factor, substance P, angiotensin II, bradykinin, somatostatin, neuromedins B and C, and litorin. The peptidase exhibited optimal activity at pH 7.5 and a Km in the micromolar range. No cleavage was produced in vasopressin, ocytocin, minigastrin I, and [Leu5]enkephalin, which include in their sequence an Xaa-Phe, Xaa-Leu, or Xaa-Ile motif. The endopeptidase activity was inhibited by divalent cation chelators and by phosphoramidon only at high concentrations (IC50 = 50 microM), whereas it was insensitive to classical inhibitors of chymotrypsin, angiotensin convertase, and serine and cysteine peptidases, as well as carboxypeptidases. It is hypothesized that this enzyme, which is distinct from neutral endopeptidase (EC 3.4.24.11), constitutes the prototype of a family of related metalloendopeptidases that inactivate peptide substrates by cleavage at the Xaa-Phe, Xaa-Leu, or Xaa-Ile bond.
Almenoff,
Biochemical and immunological properties of a membrane-bound brain metalloendopeptidase: comparison with thermolysin-like kidney neutral metalloendopeptidase.
1984, Pubmed
Almenoff,
Biochemical and immunological properties of a membrane-bound brain metalloendopeptidase: comparison with thermolysin-like kidney neutral metalloendopeptidase.
1984,
Pubmed
Bevins,
Peptides from frog skin.
1990,
Pubmed
,
Xenbase
Bourne,
The hydrolysis of brain and atrial natriuretic peptides by porcine choroid plexus is attributable to endopeptidase-24.11.
1990,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Bulant,
Processing of thyrotropin-releasing hormone prohormone (pro-TRH) generates pro-TRH-connecting peptides. Identification and characterization of prepro-TRH-(160-169) and prepro-TRH-(178-199) in the rat nervous system.
1988,
Pubmed
Chang,
Manual micro-sequence analysis of polypeptides using dimethylaminoazobenzene isothiocyanate.
1983,
Pubmed
Darby,
Purification of a cysteine endopeptidase which is secreted with bioactive peptides from the epidermal glands of Xenopus laevis.
1991,
Pubmed
,
Xenbase
Delporte,
Characterization and regulation of atrial natriuretic peptide (ANP)-R1 receptors in the human neuroblastoma cell line NB-OK-1.
1991,
Pubmed
Devault,
Amino acid sequence of rabbit kidney neutral endopeptidase 24.11 (enkephalinase) deduced from a complementary DNA.
1987,
Pubmed
Endo,
Purification and characterization of a substance P-degrading endopeptidase from rat brain.
1988,
Pubmed
Gibson,
Novel peptide fragments originating from PGLa and the caerulein and xenopsin precursors from Xenopus laevis.
1986,
Pubmed
,
Xenbase
Johnson,
Metabolism of 125I-atrial natriuretic factor by vascular smooth muscle cells. Evidence for a peptidase that specifically removes the COOH-terminal tripeptide.
1989,
Pubmed
Johnson,
Partial characterization of a metalloendopeptidase activity produced by cultured endothelial cells that removes the COOH-terminal tripeptide from 125I-atrial natriuretic factor.
1990,
Pubmed
Jongeneel,
A unique signature identifies a family of zinc-dependent metallopeptidases.
1989,
Pubmed
Kuks,
Xenopus laevis skin Arg-Xaa-Val-Arg-Gly-endoprotease. A highly specific protease cleaving after a single arginine of a consensus sequence of peptide hormone precursors.
1989,
Pubmed
,
Xenbase
Lee,
Purification and characterisation of a membrane-bound substance-P-degrading enzyme from human brain.
1981,
Pubmed
Mizuno,
Peptide C-terminal alpha-amidating enzyme purified to homogeneity from Xenopus laevis skin.
1986,
Pubmed
,
Xenbase
Mollay,
Isolation of a dipeptidyl aminopeptidase, a putative processing enzyme, from skin secretion of Xenopus laevis.
1986,
Pubmed
,
Xenbase
Nicolas,
Solid phase synthesis of somatostatin-28 II. A new biologically active octacosapeptide from anglerfish pancreatic islets.
1986,
Pubmed
Nyberg,
Characterization of substance P(1-7) and (1-8) generating enzyme in human cerebrospinal fluid.
1984,
Pubmed
Oblin,
Metalloendopeptidase (EC 3.4.24.11) but not angiotensin converting enzyme is involved in the inactivation of substance P by synaptic membranes of the rat substantia nigra.
1989,
Pubmed
Olins,
Specific inhibitors of endopeptidase 24.11 inhibit the metabolism of atrial natriuretic peptides in vitro and in vivo.
1989,
Pubmed
Plevrakis,
Proocytocin/neurophysin convertase from bovine neurohypophysis and corpus luteum secretory granules: complete purification, structure-function relationships, and competitive inhibitor.
1989,
Pubmed
Pozsgay,
The active site of endopeptidase-24.11: substrate and inhibitor studies.
1985,
Pubmed
Resnick,
A novel endopeptidase from Xenopus that recognizes alpha-helical secondary structure.
1991,
Pubmed
,
Xenbase
Roques,
Neutral endopeptidase-24.11 inhibitors: from analgesics to antihypertensives?
1990,
Pubmed
Rugg,
Degradation of [125I]-atrial natriuretic peptide by a soluble metallopeptidase isolated from rat ventricular myocytes.
1988,
Pubmed
Schwartz,
Enkephalinase (EC 3.4.24.11) inhibitors: protection of endogenous ANF against inactivation and potential therapeutic applications.
1990,
Pubmed
Stephenson,
The metabolism of neuropeptides. Hydrolysis of peptides by the phosphoramidon-insensitive rat kidney enzyme 'endopeptidase-2' and by rat microvillar membranes.
1988,
Pubmed
Toll,
Isolation and characterization of a new atrial peptide-degrading enzyme from bovine kidney.
1991,
Pubmed
