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XB-ART-24046
Am J Physiol 1992 Feb 01;2622 Pt 1:G244-8. doi: 10.1152/ajpgi.1992.262.2.G244.
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NaCl-dependent expression of amiloride-blockable Na+ channel in Xenopus oocytes.

Asher C , Singer D , Eren R , Yeger O , Dascal N , Garty H .


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RNA was isolated from chicken lower intestine (both colon and coprodeum) and injected into Xenopus oocytes. 22Na+ fluxes measured after 1-4 days demonstrated the induction of an amiloride-blockable pathway. The Na+ transporter expressed by the exogenous RNA had a high affinity to amiloride (inhibitory constant less than 0.1 microM), but was insensitive to ethylisopropyl amiloride, i.e., it is likely to be the apical Na+ channel. Functional channels were readily expressed in oocytes injected with RNA derived from chickens fed a low-NaCl diet. On the other hand, no channel activity was detected in oocytes injected with RNA isolated from chickens fed a high-NaCl diet. Thus the previously reported regulation of transport by the dietary NaCl intake involves modulations in the level of mRNA that codes either for the Na+ channel or a posttranscriptional regulator of the channel.

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