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Expression of renal organic cation transporter in Xenopus laevis oocytes.
Hori R
,
Hirai M
,
Katsura T
,
Takano M
,
Yasuhara M
,
Kaneko S
,
Satoh M
.
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The expression of the organic cation transport system of rat renal proximal tubules has been studied in Xenopus laevis oocytes injected with poly(A)+ RNA from the rat renal cortex. The effectiveness of the technique was confirmed by examining expression of the Na+/D-glucose co-transporter. Compared with water-injected and non-injected oocytes, the injection of total poly(A)+ RNA resulted in about a 3-fold increase in tetraethylammonium (TEA) uptake activity. TEA uptake by poly(A)(+)-RNA-injected oocytes was time-dependent and was inhibited by cimetidine and HgCl2, but not by p-aminohippurate. After size-fractionation on a sucrose density gradient, a 1.4-2.4 kb poly(A)+ RNA fragment was identified that expressed the organic cation transport system in oocytes. These results demonstrate that the renal organic cation transporter was expressed in oocytes and that this expression system can provide an effective assay procedure for cloning of the organic cation transporter.
Coady,
Expression of mammalian renal transporters in Xenopus laevis oocytes.
1990, Pubmed,
Xenbase
Coady,
Expression of mammalian renal transporters in Xenopus laevis oocytes.
1990,
Pubmed
,
Xenbase
Gisclon,
Cimetidine transport in isolated luminal membrane vesicles from rabbit kidney.
1987,
Pubmed
Hagenbuch,
Expression of the hepatocyte Na+/bile acid cotransporter in Xenopus laevis oocytes.
1990,
Pubmed
,
Xenbase
Hediger,
Expression cloning and cDNA sequencing of the Na+/glucose co-transporter.
,
Pubmed
,
Xenbase
Hediger,
Expression of size-selected mRNA encoding the intestinal Na/glucose cotransporter in Xenopus laevis oocytes.
1987,
Pubmed
,
Xenbase
Hori,
Transport of p-aminohippurate, tetraethylammonium and D-glucose in renal brush border membranes from rats with acute renal failure.
1985,
Pubmed
Hori,
Inhibitory effect of diethyl pyrocarbonate on the H+/organic cation antiport system in rat renal brush-border membranes.
1989,
Pubmed
Hori,
Effect of sulfhydryl reagents on tetraethylammonium transport in rat renal brush border membranes.
1987,
Pubmed
Inui,
H+-gradient-dependent active transport of tetraethylammonium cation in apical-membrane vesicles isolated from kidney epithelial cell line LLC-PK1.
1985,
Pubmed
Inui,
H+ gradient-dependent transport of aminocephalosporins in rat renal brush border membrane vesicles: role of H+/organic cation antiport system.
1985,
Pubmed
Kinsella,
Transport of organic ions in renal cortical luminal and antiluminal membrane vesicles.
1979,
Pubmed
Maegawa,
pH sensitivity of H+/organic cation antiport system in rat renal brush-border membranes.
1988,
Pubmed
McKinney,
Cimetidine transport in rabbit renal cortical brush-border membrane vesicles.
1987,
Pubmed
Miyamoto,
Functional expression of the intestinal peptide-proton co-transporter in Xenopus laevis oocytes.
1991,
Pubmed
,
Xenbase
Montrose-Rafizadeh,
Carrier-mediated transport of tetraethylammonium across rabbit renal basolateral membrane.
1989,
Pubmed
Palacin,
Expression of rat liver Na+/L-alanine co-transport in Xenopus laevis oocytes. Effect of glucagon in vivo.
1990,
Pubmed
,
Xenbase
Rafizadeh,
Tetraethylammonium transport in renal brush border membrane vesicles of the rabbit.
1987,
Pubmed
Sigel,
Use of Xenopus oocytes for the functional expression of plasma membrane proteins.
1990,
Pubmed
,
Xenbase
Sokol,
N,N'-dicyclohexylcarbodiimide inactivates organic cation transport in renal brush border membranes.
1987,
Pubmed
Sokol,
Mechanism of organic cation transport in rabbit renal basolateral membrane vesicles.
1990,
Pubmed
Sokol,
Proton-coupled organic cation transport in renal brush-border membrane vesicles.
1988,
Pubmed
Sokol,
Essential disulfide and sulfhydryl groups for organic cation transport in renal brush-border membranes.
1986,
Pubmed
Takano,
Cimetidine transport in rat renal brush border and basolateral membrane vesicles.
1985,
Pubmed
Takano,
Carrier-mediated transport systems of tetraethylammonium in rat renal brush-border and basolateral membrane vesicles.
1984,
Pubmed
Takumi,
Cloning of a membrane protein that induces a slow voltage-gated potassium current.
1988,
Pubmed
,
Xenbase
Tarnuzzer,
Expression of the mammalian system A neutral amino acid transporter in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Wright,
Transport of tetraethylammonium by rabbit renal brush-border and basolateral membrane vesicles.
1987,
Pubmed
