Campbell FE and Setzer DR (1992), Transcription termination by RNA polymerase III...

XB-ART-23740

Mol Cell Biol 1992 May 01;125:2260-72. doi: 10.1128/mcb.12.5.2260-2272.1992.

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Transcription termination by RNA polymerase III: uncoupling of polymerase release from termination signal recognition.

Campbell FE , Setzer DR .

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Xenopus RNA polymerase III specifically initiates transcription on poly(dC)-tailed DNA templates in the absence of other class III transcription factors normally required for transcription initiation. In experimental analyses of transcription termination using DNA fragments with a 5S rRNA gene positioned downstream of the tailed end, only 40% of the transcribing polymerase molecules terminate at the normally efficient Xenopus borealis somatic-type 5S rRNA terminators; the remaining 60% read through these signals and give rise to runoff transcripts. We find that the nascent RNA strand is inefficiently displaced from the DNA template during transcription elongation. Interestingly, only polymerases synthesizing a displaced RNA terminate at the 5S rRNA gene terminators; when the nascent RNA is not displaced from the template, read-through transcripts are synthesized. RNAs with 3' ends at the 5S rRNA gene terminators are judged to result from authentic termination events on the basis of multiple criteria, including kinetic properties, the precise 3' ends generated, release of transcripts from the template, and recycling of the polymerase. Even though only 40% of the polymerase molecules ultimately terminate at either of the tandem 5S rRNA gene terminators, virtually all polymerases pause there, demonstrating that termination signal recognition can be experimentally uncoupled from polymerase release. Thus, termination is dependent on RNA strand displacement during transcription elongation, whereas termination signal recognition is not. We interpret our results in terms of a two-step model for transcription termination in which polymerase release is dependent on the fate of the nascent RNA strand during transcription elongation.

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References [+] :

Allison, Effects of alterations in the 3' flanking sequence on in vivo and in vitro expression of the yeast SUP4-o tRNATyr gene. 1985, Pubmed

Allison, Effects of alterations in the 3' flanking sequence on in vivo and in vitro expression of the yeast SUP4-o tRNATyr gene. 1985, Pubmed
Bieker, Formation of a rate-limiting intermediate in 5S RNA gene transcription. 1985, Pubmed , Xenbase
Birkenmeier, A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes. 1978, Pubmed , Xenbase
Bogenhagen, Stable transcription complexes of Xenopus 5S RNA genes: a means to maintain the differentiated state. 1982, Pubmed , Xenbase
Bogenhagen, Nucleotide sequences in Xenopus 5S DNA required for transcription termination. 1981, Pubmed , Xenbase
Campbell, Displacement of Xenopus transcription factor IIIA from a 5S rRNA gene by a transcribing RNA polymerase. 1991, Pubmed , Xenbase
Coulter, A mutation in the largest subunit of RNA polymerase II alters RNA chain elongation in vitro. 1985, Pubmed
Cozzarelli, Purified RNA polymerase III accurately and efficiently terminates transcription of 5S RNA genes. 1983, Pubmed , Xenbase
Darby, Transcription complexes that program Xenopus 5S RNA genes are stable in vivo. 1988, Pubmed , Xenbase
Dedrick, Studies on transcription of 3'-extended templates by mammalian RNA polymerase II. Parameters that affect the initiation and elongation reactions. 1985, Pubmed
Dedrick, Purified RNA polymerase II recognizes specific termination sites during transcription in vitro. 1987, Pubmed
Denis, [Biochemical research on oogenesis. 7. Synthesis and maturation of 5S RNA in the small oocytes of Xenopus laevis]. 1973, Pubmed , Xenbase
Emerson, DNA sequences and transcription factor interactions of active and inactive forms of mammalian 5 S RNA genes. 1984, Pubmed , Xenbase
Engelke, Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes. 1980, Pubmed , Xenbase
Fradkin, Human transcription factor TFIIIC2 specifically interacts with a unique sequence in the Xenopus laevis 5S rRNA gene. 1989, Pubmed , Xenbase
Geiduschek, Transcription by RNA polymerase III. 1988, Pubmed
Gottlieb, Function of the mammalian La protein: evidence for its action in transcription termination by RNA polymerase III. 1989, Pubmed
Gottlieb, The RNA binding protein La influences both the accuracy and the efficiency of RNA polymerase III transcription in vitro. 1989, Pubmed
Hess, End-to-end transcription of an Alu family repeat. A new type of polymerase-III-dependent terminator and its evolutionary implication. 1985, Pubmed
James, The RET1 gene of yeast encodes the second-largest subunit of RNA polymerase III. Structural analysis of the wild-type and ret1-1 mutant alleles. 1991, Pubmed
James, ret1-1, a yeast mutant affecting transcription termination by RNA polymerase III. 1990, Pubmed
Kadesch, Studies of in vitro transcription by calf thymus RNA polymerase II using a novel duplex DNA template. 1982, Pubmed
Kane, Studies on transcription of 3'-extended DNA templates by mammalian RNA polymerase II. Partial purification and characterization of a factor from HeLa cells that facilitates renaturation of the DNA template. 1985, Pubmed
Kane, Renaturase and ribonuclease H: a novel mechanism that influences transcript displacement by RNA polymerase II in vitro. 1988, Pubmed
Kassavetis, Pausing and termination of transcription within the early region of bacteriophage T7 DNA in vitro. 1981, Pubmed
Kassavetis, S. cerevisiae TFIIIB is the transcription initiation factor proper of RNA polymerase III, while TFIIIA and TFIIIC are assembly factors. 1990, Pubmed
Kerppola, Analysis of the signals for transcription termination by purified RNA polymerase II. 1990, Pubmed
Kerppola, Intrinsic sites of transcription termination and pausing in the c-myc gene. 1988, Pubmed , Xenbase
Lassar, Transcription of class III genes: formation of preinitiation complexes. 1983, Pubmed , Xenbase
Lau, RNA polymerase pausing and transcript release at the lambda tR1 terminator in vitro. 1983, Pubmed
Matsumoto, Characterization of a new termination signal for RNA polymerase III responsible for generation of a discrete-sized RNA transcribed from salmon total genomic DNA in a HeLa cell extract. 1989, Pubmed
Mazabraud, Structure and transcription termination of a lysine tRNA gene from Xenopus laevis. 1987, Pubmed , Xenbase
Miller, Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes. 1985, Pubmed , Xenbase
Morgan, Rho-dependent termination of transcription. II. Kinetics of mRNA elongation during transcription from the bacteriophage lambda PR promoter. 1983, Pubmed
Morgan, Specificity of release by Escherichia coli transcription termination factor rho of nascent mRNA transcripts initiated at the lambda PR. 1984, Pubmed
Platt, Transcription termination and the regulation of gene expression. 1986, Pubmed
Price, Fractionation of transcription factors for RNA polymerase II from Drosophila Kc cell nuclear extracts. 1987, Pubmed
Price, Dynamic interaction between a Drosophila transcription factor and RNA polymerase II. 1989, Pubmed
Reinberg, Factors involved in specific transcription by mammalian RNA polymerase II. Transcription factor IIS stimulates elongation of RNA chains. 1987, Pubmed
Reines, Transcription elongation factor SII (TFIIS) enables RNA polymerase II to elongate through a block to transcription in a human gene in vitro. 1989, Pubmed
Reines, Identification of intrinsic termination sites in vitro for RNA polymerase II within eukaryotic gene sequences. 1987, Pubmed
Segall, Multiple factors are required for the accurate transcription of purified genes by RNA polymerase III. 1980, Pubmed
Setzer, Formation and stability of the 5 S RNA transcription complex. 1985, Pubmed , Xenbase
Shastry, Multiple factors involved in the transcription of class III genes in Xenopus laevis. 1982, Pubmed , Xenbase
Sluder, Elongation by Drosophila RNA polymerase II. Transcription of 3'-extended DNA templates. 1988, Pubmed
Sluder, Properties of a Drosophila RNA polymerase II elongation factor. 1989, Pubmed
St Clair, Quantitative immunoassay of anti-La antibodies using purified recombinant La antigen. 1988, Pubmed
Tomizawa, Factor-independent termination of transcription in a stretch of deoxyadenosine residues in the template DNA. 1987, Pubmed
Watson, Specific termination of in vitro transcription by calf thymus RNA polymerase III. 1984, Pubmed
Wolffe, A bacteriophage RNA polymerase transcribes through a Xenopus 5S RNA gene transcription complex without disrupting it. 1986, Pubmed , Xenbase
Wolffe, Developmental regulation of two 5S ribosomal RNA genes. 1988, Pubmed , Xenbase
Young, A class III transcription factor composed of RNA. 1991, Pubmed