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EMBO J
1992 Aug 01;118:3099-103. doi: 10.1002/j.1460-2075.1992.tb05382.x.
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Alternative polyadenylation of the amyloid protein precursor mRNA regulates translation.
de Sauvage F
,
Kruys V
,
Marinx O
,
Huez G
,
Octave JN
.
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The sequence of several cDNAs encoding the amyloid protein precursor showed that two polyadenylation sites of the mRNA are utilized; RNA blot analysis with different riboprobes indicated that this explains the difference between the two major 3.2 and 3.4 kb mRNAs found in the human brain. These two mRNAs, which contain the whole sequence of the natural molecules, were synthesized by in vitro transcription and translated in Xenopus oocytes. The long mRNA using the second polyadenylation site produced more protein than the short mRNA. The sequence contained within the two polyadenylation sites used in the 3' untranslated region of the amyloid protein precursor mRNA was also able to increase the production of the chicken lysozyme or the chloramphenicol acetyl transferase, as demonstrated by in vivo translation of different chimeric mRNAs obtained by in vitro transcription. This difference in protein production was also observed when chimeric cDNA constructs were transfected into Chinese hamster ovary cells. Since long mRNAs are not more stable than short mRNAs, the sequence contained within the two polyadenylation sites of the amyloid protein precursor mRNA increases the translation.
Casey,
Iron-responsive elements: regulatory RNA sequences that control mRNA levels and translation.
1988, Pubmed
Casey,
Iron-responsive elements: regulatory RNA sequences that control mRNA levels and translation.
1988,
Pubmed
Chartier-Harlin,
Early-onset Alzheimer's disease caused by mutations at codon 717 of the beta-amyloid precursor protein gene.
1991,
Pubmed
Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed
Cohen,
In situ hybridization of nucleus basalis neurons shows increased beta-amyloid mRNA in Alzheimer disease.
1988,
Pubmed
Eperon,
M13 vectors with T7 polymerase promoters: transcription limited by oligonucleotides.
1986,
Pubmed
Esch,
Cleavage of amyloid beta peptide during constitutive processing of its precursor.
1990,
Pubmed
Estus,
Potentially amyloidogenic, carboxyl-terminal derivatives of the amyloid protein precursor.
1992,
Pubmed
Glenner,
Alzheimer's disease: initial report of the purification and characterization of a novel cerebrovascular amyloid protein.
1984,
Pubmed
Goate,
Segregation of a missense mutation in the amyloid precursor protein gene with familial Alzheimer's disease.
1991,
Pubmed
Golde,
Processing of the amyloid protein precursor to potentially amyloidogenic derivatives.
1992,
Pubmed
Gorman,
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
1982,
Pubmed
Gurdon,
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
1971,
Pubmed
,
Xenbase
Han,
Endotoxin-responsive sequences control cachectin/tumor necrosis factor biosynthesis at the translational level.
1990,
Pubmed
Higgins,
Differential regulation of amyloid-beta-protein mRNA expression within hippocampal neuronal subpopulations in Alzheimer disease.
1988,
Pubmed
Huez,
Translational stability of plant viral RNAs microinjected into living cells. Influence of a 3'-poly(A) segment.
1983,
Pubmed
,
Xenbase
Johnson,
Relation of neuronal APP-751/APP-695 mRNA ratio and neuritic plaque density in Alzheimer's disease.
1990,
Pubmed
Kang,
The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptor.
,
Pubmed
Kitaguchi,
Novel precursor of Alzheimer's disease amyloid protein shows protease inhibitory activity.
1988,
Pubmed
Kruys,
Translational blockade imposed by cytokine-derived UA-rich sequences.
1989,
Pubmed
,
Xenbase
Kruys,
Identification of a translation inhibitory element (TIE) in the 3' untranslated region of the human interferon-beta mRNA.
1988,
Pubmed
,
Xenbase
Kruys,
The 3' untranslated region of the human interferon-beta mRNA has an inhibitory effect on translation.
1987,
Pubmed
,
Xenbase
Kruys,
Constitutive activity of the tumor necrosis factor promoter is canceled by the 3' untranslated region in nonmacrophage cell lines; a trans-dominant factor overcomes this suppressive effect.
1992,
Pubmed
Levy,
Mutation of the Alzheimer's disease amyloid gene in hereditary cerebral hemorrhage, Dutch type.
1990,
Pubmed
Masters,
Amyloid plaque core protein in Alzheimer disease and Down syndrome.
1985,
Pubmed
Murrell,
A mutation in the amyloid precursor protein associated with hereditary Alzheimer's disease.
1991,
Pubmed
Neve,
Expression of the Alzheimer amyloid precursor gene transcripts in the human brain.
1988,
Pubmed
Neve,
The Alzheimer amyloid precursor-related transcript lacking the beta/A4 sequence is specifically increased in Alzheimer's disease brain.
1990,
Pubmed
Palmert,
Amyloid protein precursor messenger RNAs: differential expression in Alzheimer's disease.
1988,
Pubmed
Podlisny,
Gene dosage of the amyloid beta precursor protein in Alzheimer's disease.
1987,
Pubmed
Ponte,
A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors.
1988,
Pubmed
Rouault,
Structural relationship between an iron-regulated RNA-binding protein (IRE-BP) and aconitase: functional implications.
1991,
Pubmed
Rumble,
Amyloid A4 protein and its precursor in Down's syndrome and Alzheimer's disease.
1989,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Strickland,
Antisense RNA directed against the 3' noncoding region prevents dormant mRNA activation in mouse oocytes.
1988,
Pubmed
Tanzi,
Protease inhibitor domain encoded by an amyloid protein precursor mRNA associated with Alzheimer's disease.
1988,
Pubmed
Thomas,
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
1980,
Pubmed
Van Broeckhoven,
Amyloid beta protein precursor gene and hereditary cerebral hemorrhage with amyloidosis (Dutch).
1990,
Pubmed
Vitek,
Absence of mutation in the beta-amyloid cDNAs cloned from the brains of three patients with sporadic Alzheimer's disease.
1988,
Pubmed
Young,
Efficient isolation of genes by using antibody probes.
1983,
Pubmed
Zuker,
Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information.
1981,
Pubmed
de Sauvage,
A novel mRNA of the A4 amyloid precursor gene coding for a possibly secreted protein.
1989,
Pubmed
