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A GTPase controls cell-substrate adhesion in Xenopus XTC fibroblasts.
Symons MH
,
Mitchison TJ
.
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Cell-substrate adhesion is crucial at various stages of development and for the maintenance of normal tissues. Little is known about the regulation of these adhesive interactions. To investigate the role of GTPases in the control of cell morphology and cell-substrate adhesion we have injected guanine nucleotide analogs into Xenopus XTC fibroblasts. Injection of GTP gamma S inhibited ruffling and increased spreading, suggesting an increase in adhesion. To further investigate this, we made use of GRGDSP, a peptide which inhibits binding of integrins to vitronectin and fibronectin. XTC fibroblasts injected with non-hydrolyzable analogs of GTP took much more time to round up than mock-injected cells in response to treatment with GRGDSP, while GDP beta S-injected cells rounded up in less time than controls. Injection with GTP gamma S did not inhibit cell rounding induced by trypsin however, showing that cell contractility is not significantly affected by the activation of GTPases. These data provide evidence for the existence of a GTPase which can control cell-substrate adhesion from the cytoplasm. Treatment of XTC fibroblasts with the phorbol ester 12-o-tetradecanoylphorbol-13-acetate reduced cell spreading and accelerated cell rounding in response to GRGDSP, which is essentially opposite to the effect exerted by non-hydrolyzable GTP analogs. These results suggest the existence of at least two distinct pathways controlling cell-substrate adhesion in XTC fibroblasts, one depending on a GTPase and another one involving protein kinase C.
Adams,
Changes in keratinocyte adhesion during terminal differentiation: reduction in fibronectin binding precedes alpha 5 beta 1 integrin loss from the cell surface.
1990, Pubmed
Adams,
Changes in keratinocyte adhesion during terminal differentiation: reduction in fibronectin binding precedes alpha 5 beta 1 integrin loss from the cell surface.
1990,
Pubmed
Aznavoorian,
Signal transduction for chemotaxis and haptotaxis by matrix molecules in tumor cells.
1990,
Pubmed
Bar-Sagi,
Induction of membrane ruffling and fluid-phase pinocytosis in quiescent fibroblasts by ras proteins.
1986,
Pubmed
Bender,
Multicopy suppression of the cdc24 budding defect in yeast by CDC42 and three newly identified genes including the ras-related gene RSR1.
1989,
Pubmed
Bengtsson,
Involvement of GTP-binding proteins in actin polymerization in human neutrophils.
1990,
Pubmed
Bourne,
The GTPase superfamily: a conserved switch for diverse cell functions.
1990,
Pubmed
Brass,
GTP-binding proteins and platelet activation.
1991,
Pubmed
Burridge,
Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton.
1988,
Pubmed
Cande,
Regulation of contraction and thick filament assembly-disassembly in glycerinated vertebrate smooth muscle cells.
1983,
Pubmed
Chant,
Genetic control of bud site selection in yeast by a set of gene products that constitute a morphogenetic pathway.
1991,
Pubmed
Chen,
Mechanism of retraction of the trailing edge during fibroblast movement.
1981,
Pubmed
Dedhar,
Integrins and tumor invasion.
1990,
Pubmed
DiMilla,
Mathematical model for the effects of adhesion and mechanics on cell migration speed.
1991,
Pubmed
Dodd,
Axon guidance and the patterning of neuronal projections in vertebrates.
1988,
Pubmed
,
Xenbase
Doherty,
Morphoregulatory activities of NCAM and N-cadherin can be accounted for by G protein-dependent activation of L- and N-type neuronal Ca2+ channels.
1991,
Pubmed
Downey,
Actin assembly in electropermeabilized neutrophils: role of G-proteins.
1989,
Pubmed
Dustin,
T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1.
1989,
Pubmed
Eckstein,
Guanosine 5'-O-(2-thiodiphosphate). An inhibitor of adenylate cyclase stimulation by guanine nucleotides and fluoride ions.
1979,
Pubmed
Edelman,
Morphoregulation.
1992,
Pubmed
Giancotti,
Elevated levels of the alpha 5 beta 1 fibronectin receptor suppress the transformed phenotype of Chinese hamster ovary cells.
1990,
Pubmed
Gilman,
G proteins: transducers of receptor-generated signals.
1987,
Pubmed
Gingell,
How do cells sense and respond to adhesive contacts? Diffusion-trapping of laterally mobile membrane proteins at maturing adhesions may initiate signals leading to local cytoskeletal assembly response and lamella formation.
1992,
Pubmed
Goud,
Small GTP-binding proteins and their role in transport.
1991,
Pubmed
Hall,
The cellular functions of small GTP-binding proteins.
1990,
Pubmed
Hayman,
Vitronectin--a major cell attachment-promoting protein in fetal bovine serum.
1985,
Pubmed
Hayman,
Detachment of cells from culture substrate by soluble fibronectin peptides.
1985,
Pubmed
Hibbs,
Regulation of adhesion of ICAM-1 by the cytoplasmic domain of LFA-1 integrin beta subunit.
1991,
Pubmed
Hynes,
Contact and adhesive specificities in the associations, migrations, and targeting of cells and axons.
1992,
Pubmed
Hynes,
Integrins: versatility, modulation, and signaling in cell adhesion.
1992,
Pubmed
Johnson,
Molecular characterization of CDC42, a Saccharomyces cerevisiae gene involved in the development of cell polarity.
1990,
Pubmed
Kahn,
Fluoride is not an activator of the smaller (20-25 kDa) GTP-binding proteins.
1991,
Pubmed
Kawata,
A novel small molecular weight GTP-binding protein with the same putative effector domain as the ras proteins in bovine brain membranes. Purification, determination of primary structure, and characterization.
1988,
Pubmed
Kitayama,
A ras-related gene with transformation suppressor activity.
1989,
Pubmed
Lamb,
Regulation of actin microfilament integrity in living nonmuscle cells by the cAMP-dependent protein kinase and the myosin light chain kinase.
1988,
Pubmed
Leone,
Reduced tumor incidence, metastatic potential, and cytokine responsiveness of nm23-transfected melanoma cells.
1991,
Pubmed
Lester,
G-protein involvement in matrix-mediated motility and invasion of high and low experimental metastatic B16 melanoma clones.
1989,
Pubmed
Liotta,
Tumor invasion and metastases--role of the extracellular matrix: Rhoads Memorial Award lecture.
1986,
Pubmed
Munemitsu,
Molecular cloning and expression of a G25K cDNA, the human homolog of the yeast cell cycle gene CDC42.
1990,
Pubmed
Neugebauer,
Cell-surface regulation of beta 1-integrin activity on developing retinal neurons.
1991,
Pubmed
Nishizuka,
Studies and perspectives of protein kinase C.
1986,
Pubmed
O'Toole,
Modulation of the affinity of integrin alpha IIb beta 3 (GPIIb-IIIa) by the cytoplasmic domain of alpha IIb.
1991,
Pubmed
O'Toole,
Affinity modulation of the alpha IIb beta 3 integrin (platelet GPIIb-IIIa) is an intrinsic property of the receptor.
1990,
Pubmed
Otero,
Transphosphorylation and G protein activation.
1990,
Pubmed
Otero,
Activation of muscarinic potassium currents by ATP gamma S in atrial cells.
1988,
Pubmed
Pardi,
Antigen-receptor complex stimulation triggers protein kinase C-dependent CD11a/CD18-cytoskeleton association in T lymphocytes.
1992,
Pubmed
Paterson,
Microinjection of recombinant p21rho induces rapid changes in cell morphology.
1990,
Pubmed
Phillips,
GPIIb-IIIa: the responsive integrin.
1991,
Pubmed
Pierschbacher,
Influence of stereochemistry of the sequence Arg-Gly-Asp-Xaa on binding specificity in cell adhesion.
1987,
Pubmed
Pizon,
Human cDNAs rap1 and rap2 homologous to the Drosophila gene Dras3 encode proteins closely related to ras in the 'effector' region.
1988,
Pubmed
Sandoval,
Role of nucleotides in tubulin polymerization: effect of guanylyl 5'-methylenediphosphonate.
1977,
Pubmed
Sawin,
Poleward microtubule flux mitotic spindles assembled in vitro.
1991,
Pubmed
,
Xenbase
Sha'afi,
Activation of the neutrophil.
1988,
Pubmed
Shattil,
Induction of the fibrinogen receptor on human platelets by intracellular mediators.
1987,
Pubmed
Shaw,
The activation dependent adhesion of macrophages to laminin involves cytoskeletal anchoring and phosphorylation of the alpha 6 beta 1 integrin.
1990,
Pubmed
Shinjo,
Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42.
1990,
Pubmed
Simon,
Diversity of G proteins in signal transduction.
1991,
Pubmed
Small,
The actin cytoskeleton.
1988,
Pubmed
Springer,
The sensation and regulation of interactions with the extracellular environment: the cell biology of lymphocyte adhesion receptors.
1990,
Pubmed
Svitkina,
Actin cytoskeleton of spread fibroblasts appears to assemble at the cell edges.
1986,
Pubmed
Symons,
Control of actin polymerization in live and permeabilized fibroblasts.
1991,
Pubmed
Särndahl,
Association of ligand-receptor complexes with actin filaments in human neutrophils: a possible regulatory role for a G-protein.
1989,
Pubmed
Therrien,
Guanine nucleotide-induced polymerization of actin in electropermeabilized human neutrophils.
1989,
Pubmed
Wieland,
Receptor-regulated formation of GTP[gamma S] with subsequent persistent Gs-protein activation in membranes of human platelets.
1989,
Pubmed
Zetter,
Cell motility and the extracellular matrix.
1990,
Pubmed
Zetter,
Effects of protease treatment on growth, morphology, adhesion, and cell surface proteins of secondary chick embryo fibroblasts.
1976,
Pubmed
van Kooyk,
Enhancement of LFA-1-mediated cell adhesion by triggering through CD2 or CD3 on T lymphocytes.
1989,
Pubmed
