Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Influence of apical Na+ entry on Na(+)-K(+)-ATPase in amphibian distalnephron cells in culture.
Lyoussi B
,
Crabbé J
.
???displayArticle.abstract???
1. Transepithelial Na+ transport, Na(+)-K(+)-ATPase activity and ouabain binding were measured in cells originating from the distal part of amphibian nephron (A6) which form 'tight' monolayers in culture, under standard (control) incubation conditions and after various manoeuvres designed to reversibly interfere with Na+ transport. 2. At spontaneous transport rate, the short-circuit current (which reflects transepithelial Na+ transport) and the Na(+)-K(+)-ATPase activity averaged 7.0 microA/cm2 and 5.9 mumol Pi/(mg protein.h), respectively (n = 53). Short-circuit current and Na(+)-K(+)-ATPase activity appeared to be directly related over a wide range. 3. Suppression of Na+ transport led to a progressive decrease in Na(+)-K(+)-ATPase activity over several hours, with an apparent half-life of approximately 6 h after subtraction of baseline enzyme activity. 4. When A6 cells were allowed to resume sodium transport, the short-circuit current and Na(+)-K(+)-ATPase activity returned to control levels within 12-24 h, the former recovering somewhat faster. 5. When apical sodium concentration was reduced, a decrease in enzyme level occurred inasmuch as short-circuit current decreased. 6. There was good agreement between the measured enzyme activity and ouabain binding onto dispersed A6 cells, which suggests that it is unlikely that the changes observed result from internalization vs. insertion in the plasma membrane of sodium pumps.
Barlet-Bas,
Sodium-independent in vitro induction of Na+,K+-ATPase by aldosterone in renal target cells: permissive effect of triiodothyronine.
1988, Pubmed
Barlet-Bas,
Sodium-independent in vitro induction of Na+,K+-ATPase by aldosterone in renal target cells: permissive effect of triiodothyronine.
1988,
Pubmed
Bindels,
Stimulation of sodium transport by aldosterone and arginine vasotocin in A6 cells.
1988,
Pubmed
,
Xenbase
Bowen,
Pretranslational regulation of Na-K-ATPase in cultured canine kidney cells by low K+.
1987,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Duncan,
Corticosterone's metabolite is an agonist for Na+ transport stimulation in A6 cells.
1988,
Pubmed
Eaton,
Intracellular sodium ion activity and sodium transport in rabbit urinary bladder.
1981,
Pubmed
El Mernissi,
Specific activity of Na-K-ATPase after adrenalectomy and hormone replacement along the rabbit nephron.
1984,
Pubmed
Forbush,
Assay of Na,K-ATPase in plasma membrane preparations: increasing the permeability of membrane vesicles using sodium dodecyl sulfate buffered with bovine serum albumin.
1983,
Pubmed
Granitzer,
Apical and basolateral conductance in cultured A6 cells.
1991,
Pubmed
,
Xenbase
Johnson,
Hormonal regulation of Na+-K+-ATPase in cultured epithelial cells.
1986,
Pubmed
Lamb,
Regulation of the abundance of sodium pumps in isolated animal cells.
1990,
Pubmed
Leal,
Effects of aldosterone on (Na+ + K+)-ATPase of amphibian sodium-transporting epithelial cells (A6) in culture.
1989,
Pubmed
,
Xenbase
Li,
The role of sodium-channel density in the natriferic response of the toad urinary bladder to an antidiuretic hormone.
1982,
Pubmed
Paccolat,
Aldosterone regulation of Na+ transport and Na+-K+-ATPase in A6 cells: role of growth conditions.
1987,
Pubmed
Palmer,
Aldosterone control of the density of sodium channels in the toad urinary bladder.
1982,
Pubmed
Perkins,
Transport properties of toad kidney epithelia in culture.
1981,
Pubmed
,
Xenbase
Pressley,
Ion concentration-dependent regulation of Na,K-pump abundance.
1988,
Pubmed
Wills,
Intracellular Na+ activity as a function of Na+ transport rate across a tight epithelium.
1980,
Pubmed
Wills,
Amiloride-sensitive Na+ transport across cultured renal (A6) epithelium: evidence for large currents and high Na:K selectivity.
1990,
Pubmed
,
Xenbase