XB-ART-23337
Sheng Li Xue Bao
1992 Oct 01;445:470-7.
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[The expression of kainate and GABA receptors in oocytes of Bufo bufo gargarizans].
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Oocytes of Bufo bufo gargarizans were used as an expression system for analyzing structure of exogenous membrane protein and their functions. Poly (A)+ mRNA isolated from rat brain was injected into Toad (Bufo bufo gargarizans) oocytes (50 ng/oocyte). Rat brain kainic acid and gamma-aminobutyric acid(GABA) receptors expressed by the injected mRNA were integrated in the oocyte membrane 48 h after the injection at 19 degrees C. Membrane currents induced by kainic acid (5 x 10(-5) mol/L) and GABA (10(-4) mol/L) were 294.0 +/- 6.4 nA (n = 5) and 309.5 +/- 4.9 nA (n = 4) respectively. The kainic acid induced current reached its maximum value at about 10(-3) mol/L. Moreover, it was observed that the 36Cl- influx of the oocytes microinjected with mRNA was one-fold more rapid than the control oocytes. These results indicate that the oocytes of Bufo bufo gargarizans as those of Xenopus laevis can be used to express membrane proteins (receptors & transports) to acquire their proper functions from exogenous mRNA.
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