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Expression and characterization of a canine hippocampal inwardly rectifying K+ current in Xenopus oocytes.
Cui J
,
Mandel G
,
DiFrancesco D
,
Kline RP
,
Pennefather P
,
Datyner NB
,
Haspel HC
,
Cohen IS
.
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1. An inwardly rectifying potassium current expressed in Xenopus laevis oocytes injected with canine hippocampal poly(A)+ RNA was investigated with the two-microelectrode voltage clamp technique. 2. Xenopus oocytes injected with canine hippocampal poly(A)+ RNA expressed a current activated by hyperpolarization. This current contained an instantaneous and a time-dependent component. Both components were inwardly rectifying and could be blocked by extracellular Cs+ or Ba2+. 3. The expressed current was carried mainly by K+. Its reversal potential measured in different [K+]os could be fitted by the Nernst equation with a slope of -50.7 per tenfold change in [K+]o. Extracellular Cl- and Na+ made minimal contributions to the current. 4. The activation of the expressed current depended on both voltage and [K+]o. Activation started near EK and the activation curve shifted along the voltage axis in parallel with EK when [K+]o was altered. 5. The activation time constants of the expressed current also depended on both voltage and [K+]o. The voltage dependence of the time constants was bell-shaped and the peak value was at a potential 30-50 mV more negative than EK. The voltage dependence of the time constants shifted along the voltage axis when EK was changed. 6. The poly(A)+ RNA extracted from canine hippocampus was fractionated in a 10-31% linear sucrose gradient. The size of the mRNA required to express the inwardly rectifying current was estimated to be around 4 kb. 7. In conclusion, the expressed current is an inwardly rectifying potassium current. The canine hippocampal mRNA should be an excellent source for expression-cloning of the inward rectifier channel.
Adrian,
Rectification in muscle membrane.
1969, Pubmed
Adrian,
Rectification in muscle membrane.
1969,
Pubmed
Auffray,
Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
1980,
Pubmed
Barish,
A transient calcium-dependent chloride current in the immature Xenopus oocyte.
1983,
Pubmed
,
Xenbase
Christie,
Expression of a cloned rat brain potassium channel in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Ciani,
A model for anomalous rectification: electrochemical-potential-dependent gating of membrane channels.
1978,
Pubmed
Cohen,
Internal and external K+ help gate the inward rectifier.
1989,
Pubmed
Constanti,
Fast inward-rectifying current accounts for anomalous rectification in olfactory cortex neurones.
1983,
Pubmed
Dascal,
Involvement of a GTP-binding protein in mediation of serotonin and acetylcholine responses in Xenopus oocytes injected with rat brain messenger RNA.
1986,
Pubmed
,
Xenbase
Dascal,
The use of Xenopus oocytes for the study of ion channels.
1987,
Pubmed
,
Xenbase
Dierks,
DNA sequences preceding the rabbit beta-globin gene are required for formation in mouse L cells of beta-globin RNA with the correct 5' terminus.
1981,
Pubmed
Frech,
A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning.
1989,
Pubmed
,
Xenbase
Goldin,
Messenger RNA coding for only the alpha subunit of the rat brain Na channel is sufficient for expression of functional channels in Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Grupe,
Cloning and expression of a human voltage-gated potassium channel. A novel member of the RCK potassium channel family.
1990,
Pubmed
,
Xenbase
Hagiwara,
The anomalous rectification and cation selectivity of the membrane of a starfish egg cell.
1974,
Pubmed
Hagiwara,
Potassium current and the effect of cesium on this current during anomalous rectification of the egg cell membrane of a starfish.
1976,
Pubmed
Halliwell,
Voltage-clamp analysis of muscarinic excitation in hippocampal neurons.
1982,
Pubmed
Harvey,
Characterization of the inward-rectifying potassium current in cat ventricular myocytes.
1988,
Pubmed
Hestrin,
The interaction of potassium with the activation of anomalous rectification in frog muscle membrane.
1981,
Pubmed
Hollmann,
Cloning by functional expression of a member of the glutamate receptor family.
1989,
Pubmed
,
Xenbase
Hoshi,
Biophysical and molecular mechanisms of Shaker potassium channel inactivation.
1990,
Pubmed
,
Xenbase
Isacoff,
Putative receptor for the cytoplasmic inactivation gate in the Shaker K+ channel.
1991,
Pubmed
,
Xenbase
Jacobson,
Purification and fractionation of poly(A)+ RNA.
1987,
Pubmed
Jentsch,
Primary structure of Torpedo marmorata chloride channel isolated by expression cloning in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Kandel,
Anomalous rectification in the metacerebral giant cells and its consequences for synaptic transmission.
1966,
Pubmed
Krafte,
Inactivation of cloned Na channels expressed in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Kurachi,
Voltage-dependent activation of the inward-rectifier potassium channel in the ventricular cell membrane of guinea-pig heart.
1985,
Pubmed
Kusano,
Cholinergic and catecholaminergic receptors in the Xenopus oocyte membrane.
1982,
Pubmed
,
Xenbase
Leech,
Inward rectification in frog skeletal muscle fibres and its dependence on membrane potential and external potassium.
1981,
Pubmed
Lewis,
Expression of an inwardly rectifying K+ channel from rat basophilic leukemia cell mRNA in Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Madison,
Phorbol esters block a voltage-sensitive chloride current in hippocampal pyramidal cells.
,
Pubmed
Masu,
cDNA cloning of bovine substance-K receptor through oocyte expression system.
,
Pubmed
,
Xenbase
Matsuda,
Triple-barrel structure of inwardly rectifying K+ channels revealed by Cs+ and Rb+ block in guinea-pig heart cells.
1989,
Pubmed
Noble,
Outward membrane currents activated in the plateau range of potentials in cardiac Purkinje fibres.
1969,
Pubmed
Noda,
Existence of distinct sodium channel messenger RNAs in rat brain.
,
Pubmed
Ohmori,
Inactivation kinetics and steady-state current noise in the anomalous rectifier of tunicate egg cell membranes.
1978,
Pubmed
Oliva,
The mechanism of rectification of iK1 in canine Purkinje myocytes.
1990,
Pubmed
Pennefather,
Origin of the potassium and voltage dependence of the cardiac inwardly rectifying K-current (IK1).
1992,
Pubmed
Saigusa,
Outward currents through the inwardly rectifying potassium channel of guinea-pig ventricular cells.
1988,
Pubmed
Sakmann,
Conductance properties of single inwardly rectifying potassium channels in ventricular cells from guinea-pig heart.
1984,
Pubmed
Singer,
The roles of the subunits in the function of the calcium channel.
1991,
Pubmed
,
Xenbase
Standen,
Potassium depletion and sodium block of potassium currents under hyperpolarization in frog sartorius muscle.
1979,
Pubmed
Sumikawa,
The molecular cloning and characterisation of cDNA coding for the alpha subunit of the acetylcholine receptor.
1982,
Pubmed
Williams,
Inward rectification of resting and opiate-activated potassium currents in rat locus coeruleus neurons.
1988,
Pubmed