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Molecular characterisation and stage-specific expression of proliferating cell nuclear antigen (PCNA) from the malarial parasite, Plasmodium falciparum.
Kilbey BJ
,
Fraser I
,
McAleese S
,
Goman M
,
Ridley RG
.
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The gene encoding the malarial homologue of proliferating cell nuclear antigen, PCNA, has been identified and characterised. It is located on chromosome 13. The coding sequence of 825 nucleotides predicts a protein of 30,586 Da. There are no introns and northern analysis reveals a transcript of approximately 1.6kb. The conserved residues which characterise the PCNAs of human, Drosophila, Saccharomyces and Xenopus are present in PfPCNA but the overall identity of PfPCNA with human and yeast PCNAs is low; 34% and 31% respectively. PfPCNA is longer than the PCNAs of these other species by about 16 amino acids, most of which are present in a block near the carboxy terminus. Antibodies against a purified PfPCNA-glutathione-S-transferase fusion protein recognise a single band in western blots of parasite extracts at 32kDa. The same antiserum has been used to demonstrate that the expression of PfPCNA is regulated during the intraerythrocytic development of the parasite. Expression increases dramatically in late trophozoites and is maintained during the subsequent nuclear divisions which produce schizonts.
Bauer,
Molecular cloning, structure and expression of the yeast proliferating cell nuclear antigen gene.
1990, Pubmed
Bauer,
Molecular cloning, structure and expression of the yeast proliferating cell nuclear antigen gene.
1990,
Pubmed
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977,
Pubmed
Bravo,
Induction of the nuclear protein 'cyclin' in quiescent mouse 3T3 cells stimulated by serum and growth factors. Correlation with DNA synthesis.
1984,
Pubmed
Bravo,
Changes in the nuclear distribution of cyclin (PCNA) but not its synthesis depend on DNA replication.
1985,
Pubmed
Bravo,
Cyclin/PCNA is the auxiliary protein of DNA polymerase-delta.
,
Pubmed
Bravo,
A search for differential polypeptide synthesis throughout the cell cycle of HeLa cells.
1980,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Delves,
Cloning of a beta-tubulin gene from Plasmodium falciparum.
1989,
Pubmed
Delves,
Expression of alpha and beta tubulin genes during the asexual and sexual blood stages of Plasmodium falciparum.
1990,
Pubmed
Devereux,
A comprehensive set of sequence analysis programs for the VAX.
1984,
Pubmed
Goman,
The establishment of genomic DNA libraries for the human malaria parasite Plasmodium falciparum and identification of individual clones by hybridisation.
1982,
Pubmed
Goman,
The complete sequence of a Plasmodium malariae SSUrRNA gene and its comparison to other plasmodial SSUrRNA genes.
1991,
Pubmed
Hall,
Antigens of the erythrocytes stages of the human malaria parasite Plasmodium falciparum detected by monoclonal antibodies.
1983,
Pubmed
Inselburg,
Synthesis of DNA during the asexual cycle of Plasmodium falciparum in culture.
1984,
Pubmed
Jaskulski,
Inhibition of cellular proliferation by antisense oligodeoxynucleotides to PCNA cyclin.
1988,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lambros,
Synchronization of Plasmodium falciparum erythrocytic stages in culture.
1979,
Pubmed
Leibovici,
Characterization and developmental expression of Xenopus proliferating cell nuclear antigen (PCNA).
1990,
Pubmed
,
Xenbase
Mathews,
Identity of the proliferating cell nuclear antigen and cyclin.
,
Pubmed
Matsumoto,
Molecular cloning of cDNA coding for rat proliferating cell nuclear antigen (PCNA)/cyclin.
1987,
Pubmed
Miyachi,
Autoantibody to a nuclear antigen in proliferating cells.
1978,
Pubmed
Prelich,
Coordinated leading and lagging strand synthesis during SV40 DNA replication in vitro requires PCNA.
1988,
Pubmed
Prelich,
Functional identity of proliferating cell nuclear antigen and a DNA polymerase-delta auxiliary protein.
,
Pubmed
Ridley,
DNA polymerase delta: gene sequences from Plasmodium falciparum indicate that this enzyme is more highly conserved than DNA polymerase alpha.
1991,
Pubmed
Robson,
A highly conserved amino-acid sequence in thrombospondin, properdin and in proteins from sporozoites and blood stages of a human malaria parasite.
1988,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Smith,
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
1988,
Pubmed
Takasaki,
A nuclear antigen associated with cell proliferation and blast transformation.
1981,
Pubmed
Takasaki,
Characterization of proliferating cell nuclear antigen recognized by autoantibodies in lupus sera.
1984,
Pubmed
Travali,
Structure of the human gene for the proliferating cell nuclear antigen.
1989,
Pubmed
Triglia,
Towards a high-resolution map of the Plasmodium falciparum genome.
1992,
Pubmed
Tsurimoto,
Replication factors required for SV40 DNA replication in vitro. II. Switching of DNA polymerase alpha and delta during initiation of leading and lagging strand synthesis.
1991,
Pubmed
Tsurimoto,
Replication factors required for SV40 DNA replication in vitro. I. DNA structure-specific recognition of a primer-template junction by eukaryotic DNA polymerases and their accessory proteins.
1991,
Pubmed
Wood,
Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.
1985,
Pubmed
Yamaguchi,
Drosophila proliferating cell nuclear antigen (cyclin) gene: structure, expression during development, and specific binding of homeodomain proteins to its 5'-flanking region.
1990,
Pubmed
Yanisch-Perron,
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
1985,
Pubmed
Zolg,
Plasmodium falciparum: modifications of the in vitro culture conditions improving parasitic yields.
1982,
Pubmed