Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Antimicrob Agents Chemother
1993 Mar 01;373:393-7. doi: 10.1128/AAC.37.3.393.
Show Gene links
Show Anatomy links
Effects of two magainin peptides on eicosanoid release from rat peritoneal macrophages.
Matera G
,
Cook JA
,
Geisel J
,
Ashton SH
,
Wise WC
,
Focá A
,
Berkowitz BA
,
Halushka PV
.
???displayArticle.abstract??? Magainins are novel polycationic peptides with broad-spectrum antimicrobial activity, including activity against gram-negative bacteria. Gram-negative bacteremia can elicit endotoxic shock that is associated with the increased formation of eicosanoids. Inhibition of eicosanoid synthesis has been shown to improve the outcome of experimental endotoxic shock. The aim of the present study was to test the in vitro effects of two magainin peptides, MSI-97 (M1) and MSI-98 (M2), on eicosanoid synthesis by rat peritoneal macrophages (M phi) stimulated by Salmonella enteritidis lipopolysaccharide (LPS; 50 micrograms/ml) and Salmonella minnesota lipid A (5 micrograms/ml) and to compare their effects on LPS reactivity with a metachromatic dye. M1 (100 micrograms/ml) significantly (P < 0.05) reduced LPS-stimulated synthesis of thromboxane B2 (TXB2), without changing 6-keto-prostaglandin F1 alpha in M phi. Similarly, M2 (10 micrograms/ml) significantly attenuated M phi synthesis of TXB2 stimulated by either LPS or lipid A. However, at a higher concentration (100 micrograms/ml), M2 but not M1 significantly augmented LPS-induced increases in TXB2 and 6-keto-prostaglandin F1 alpha. Polymyxin B (40 micrograms/ml) inhibited LPS production and lipid A-stimulated TXB2 production. M1 (100 micrograms/ml) and polymyxin B (10 and 40 micrograms/ml) also inhibited calcium ionophore A23187 (10 microM)-induced synthesis of TXB2. The lipid A moiety of LPS reacts with dimethylmethylene blue dye, providing a metachromatic assay of LPS. This metachromatic reaction with lipid A was significantly reduced by polymyxin B and M2 at all concentrations. M1 was effective only at the highest M1:lipid A concentration ratio (2:1). Thus, M1 and M2 share some similarities with polymyxin B in inhibiting lipid A reactivity with the dye, which suggests that these magainins may also bind to lipid A. However, M1 was devoid of any inhibitory effects on dye reactivity with S. enteritidis LPS and M2 was inhibitory at only one concentration ratio (1:5). In conclusion, the varied effects of the magainin peptides on LPS, lipid A, and M phi eicosanoid synthesis appear to depend on the type of peptide used and on its concentration.
Aderem,
Bacterial lipopolysaccharides prime macrophages for enhanced release of arachidonic acid metabolites.
1986, Pubmed
Aderem,
Bacterial lipopolysaccharides prime macrophages for enhanced release of arachidonic acid metabolites.
1986,
Pubmed
Berkowitz,
Magainins: a new family of membrane-active host defense peptides.
1990,
Pubmed
,
Xenbase
Bessalle,
Augmentation of the antibacterial activity of magainin by positive-charge chain extension.
1992,
Pubmed
,
Xenbase
Burch,
Vasopressin stimulates thromboxane synthesis in the toad urinary bladder: effects of imidazole.
1979,
Pubmed
Chen,
Synthetic magainin analogues with improved antimicrobial activity.
1988,
Pubmed
,
Xenbase
Flynn,
Polymyxin B moderates acidosis and hypotension in established, experimental gram-negative septicemia.
1987,
Pubmed
Geisel,
Endotoxin-induced arachidonic acid metabolism requires de novo protein synthesis and protein kinase C activation.
1991,
Pubmed
Juretić,
Magainin 2 amide and analogues. Antimicrobial activity, membrane depolarization and susceptibility to proteolysis.
1989,
Pubmed
,
Xenbase
Keler,
Metachromatic assay for the quantitative determination of bacterial endotoxins.
1986,
Pubmed
Lüderitz,
Structural and physicochemical requirements of endotoxins for the activation of arachidonic acid metabolism in mouse peritoneal macrophages in vitro.
1989,
Pubmed
Matera,
Beneficial effects of a 5-lipoxygenase inhibitor in endotoxic shock in the rat.
1988,
Pubmed
Matsuzaki,
Magainin 1-induced leakage of entrapped calcein out of negatively-charged lipid vesicles.
1989,
Pubmed
,
Xenbase
Mazzei,
Polymyxin B is a more selective inhibitor for phospholipid-sensitive Ca2+-dependent protein kinase than for calmodulin-sensitive Ca2+-dependent protein kinase.
1982,
Pubmed
Morrison,
Binding of polymyxin B to the lipid A portion of bacterial lipopolysaccharides.
1976,
Pubmed
Nakabayashi,
Phosphorylation of magainin-2 by protein kinase C and inhibition of protein kinase C isozymes by a synthetic analogue of magainin-2-amide.
1990,
Pubmed
,
Xenbase
Rana,
Outer membrane structure in smooth and rough strains of Salmonella typhimurium and their susceptibility to the antimicrobial peptides, magainins and defensins.
1989,
Pubmed
,
Xenbase
Rana,
Interactions between Salmonella typhimurium lipopolysaccharide and the antimicrobial peptide, magainin 2 amide.
1990,
Pubmed
,
Xenbase
Rosen,
Bacterial lipopolysaccharide regulates the phosphorylation of the 68K protein kinase C substrate in macrophages.
1989,
Pubmed
Shenep,
Kinetics of endotoxin release during antibiotic therapy for experimental gram-negative bacterial sepsis.
1984,
Pubmed
Soravia,
Antimicrobial properties of peptides from Xenopus granular gland secretions.
1988,
Pubmed
,
Xenbase
Terry,
The cDNA sequence coding for prepro-PGS (prepro-magainins) and aspects of the processing of this prepro-polypeptide.
1988,
Pubmed
,
Xenbase
Wade,
All-D amino acid-containing channel-forming antibiotic peptides.
1990,
Pubmed
,
Xenbase
Westerhoff,
Magainins and the disruption of membrane-linked free-energy transduction.
1989,
Pubmed
,
Xenbase
Wightman,
The activation of protein kinase C by biologically active lipid moieties of lipopolysaccharide.
1984,
Pubmed
Wise,
Protective effects of thromboxane synthetase inhibitors in rats in endotoxic shock.
1980,
Pubmed
Wrenn,
Dual calcium-dependent protein phosphorylation systems in pancreas and their differential regulation by polymyxin B1.
1984,
Pubmed
Zasloff,
Magainins, a class of antimicrobial peptides from Xenopus skin: isolation, characterization of two active forms, and partial cDNA sequence of a precursor.
1987,
Pubmed
,
Xenbase
Zasloff,
Antimicrobial activity of synthetic magainin peptides and several analogues.
1988,
Pubmed
,
Xenbase