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Acetylcholine reduces the slow calcium current in embryonic skeletal muscle cells in culture.
Moody-Corbett FL
,
Virgo NS
.
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Xenopus skeletal muscle cells when grown in culture develop a slow inward calcium current that is sensitive to dihydropyridines. Acetylcholine (ACh, 10 microM) applied through a puffer pipette caused a large inward current in these cells (at the holding potential) through the nicotinic receptor channels and reduced the inward calcium current (during a step depolarization to 0 mV). After the ACh application was discontinued the holding current rapidly returned to pre-ACh levels (20 s) whereas the calcium current showed a slow, partial recovery to pre-ACh levels. Outward potassium current was also reduced during the application of ACh but recovered completely after ACh was discontinued. The effect of ACh on the calcium current was not mimicked by muscarine (100 microM) and was absent when 10 micrograms/ml alpha-bungarotoxin was added to the bath suggesting that the decrease in calcium current was mediated by current through the nicotinic receptor.
Ashley,
Ca2+ and activation mechanisms in skeletal muscle.
1991, Pubmed
Ashley,
Ca2+ and activation mechanisms in skeletal muscle.
1991,
Pubmed
Bloch,
Reversible loss of acetylcholine receptor clusters at the developing rat neuromuscular junction.
1981,
Pubmed
Bloch,
Loss of acetylcholine receptor clusters induced by treatment of cultured rat myotubes with carbachol.
1986,
Pubmed
Bregestovski,
Calcium conductance of acetylcholine-induced endplate channels.
1979,
Pubmed
Brehm,
Calcium entry leads to inactivation of calcium channel in Paramecium.
1978,
Pubmed
Brehm,
Acetylcholine receptor channel properties during development of Xenopus muscle cells in culture.
1984,
Pubmed
,
Xenbase
Decker,
Calcium permeability of the nicotinic acetylcholine receptor: the single-channel calcium influx is significant.
1990,
Pubmed
Eusebi,
Acetylcholine may regulate its own nicotinic receptor-channel through the C-kinase system.
1987,
Pubmed
Fosset,
[3H]nitrendipine receptors in skeletal muscle.
1983,
Pubmed
Giovannelli,
Acetylcholine induces voltage-independent increase of cytosolic calcium in mouse myotubes.
1991,
Pubmed
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Henderson,
The absence of calcium blocks impulse-evoked release of acetylcholine but not de novo formation of functional neuromuscular synaptic contacts in culture.
1984,
Pubmed
,
Xenbase
Hochachka,
Defense strategies against hypoxia and hypothermia.
1986,
Pubmed
Jacobs,
Effect of various agents on the cytoplasmic calcium concentration in cultured human muscle cells.
1991,
Pubmed
Klepper,
Nicotinic cholinergic modulation of voltage-dependent calcium current in bovine adrenal chromaffin cells.
1990,
Pubmed
Mongini,
Free cytoplasmic Ca++ at rest and after cholinergic stimulus is increased in cultured muscle cells from Duchenne muscular dystrophy patients.
1988,
Pubmed
Moody-Corbett,
Exposure to nerve does not affect the appearance of calcium currents in embryonic muscle.
1991,
Pubmed
,
Xenbase
Moody-Corbett,
Calcium current in embryonic Xenopus muscle cells in culture.
1989,
Pubmed
,
Xenbase
Peng,
Participation of calcium and calmodulin in the formation of acetylcholine receptor clusters.
1984,
Pubmed
,
Xenbase
Sarabia,
Regulation of cytosolic Ca2+ in clonal human muscle cell cultures.
1989,
Pubmed
Wallace,
Regulation of agrin-induced acetylcholine receptor aggregation by Ca++ and phorbol ester.
1988,
Pubmed
Zhu,
Increase in intracellular calcium induced by the polycation-coated latex bead, a stimulus that causes postsynaptic-type differentiation in cultured Xenopus muscle cells.
1988,
Pubmed
,
Xenbase
