XB-ART-22479
Biochem Biophys Res Commun
1993 Jun 30;1933:1116-22. doi: 10.1006/bbrc.1993.1741.
Show Gene links
Show Anatomy links
Production of recombinant Xenopus fibroblast growth factor receptor-1 using a baculovirus expression system.
???displayArticle.abstract???
A baculovirus expression system has been used to express large quantities of full-length, biologically active Xenopus fibroblast growth factor receptor-1. Recombinant XFGFR-1 was purified to near homogeneity by a single step immunoaffinity purification procedure. The recombinant XFGFR-1 binds both FGF-1 and FGF-2 with high affinity. XFGFR-1 undergoes autophosphorylation principly on tyrosine residues in an immune-complex kinase assay. Thus, recombinant XFGFR-1 expressed in insect cells is biologically active and suitable for structural and functional analysis.
???displayArticle.pubmedLink??? 8391800
???displayArticle.link??? Biochem Biophys Res Commun
Species referenced: Xenopus
Genes referenced: fgf1 fgf2 fgfr1