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Proteolytic footprinting of transcription factor TFIIIA reveals different tightly binding sites for 5S RNA and 5S DNA.
Bogenhagen DF
.
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Transcription factor IIIA (TFIIIA) employs an array of nine N-terminal zinc fingers to bind specifically to both 5S RNA and 5S DNA. The binding of TFIIIA to 5S RNA and 5S DNA was studied by using a protease footprinting technique. Brief treatment of free or bound TFIIA with trypsin or chymotrypsin generated fragments which were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Fragments retaining the N terminus of TFIIA were identified by immunoblotting with an antibody directed against the N terminus of TFIIIA. Proteolytic footprinting of TFIIIA complexed with 5S DNA derivatives reinforced other evidence that the three N-terminal zinc fingers of TFIIIA bind most tightly to 5S DNA. Proteolytic footprinting of TFIIIA in reconstituted 7S ribonucleoprotein particles revealed different patterns of trypsin sensitivity for TFIIIA bound to oocyte versus somatic 5S RNA. Trypsin cleaved TFIIIA between zinc fingers 3 and 4 more readily when the protein was bound to somatic 5S RNA than when it was bound to oocyte 5S RNA. A tryptic fragment of TFIIIA containing zinc fingers 4 through 7 remained tightly associated with somatic 5S RNA. Zinc fingers 4 through 7 may represent a tightly binding site for 5S RNA in the same sense that fingers 1 through 3 represent a tightly binding site for 5S DNA.
Bieker,
Physical properties and DNA-binding stoichiometry of a 5 S gene-specific transcription factor.
1984, Pubmed,
Xenbase
Bieker,
Physical properties and DNA-binding stoichiometry of a 5 S gene-specific transcription factor.
1984,
Pubmed
,
Xenbase
Bogenhagen,
The intragenic control region of the Xenopus 5 S RNA gene contains two factor A binding domains that must be aligned properly for efficient transcription initiation.
1985,
Pubmed
,
Xenbase
Bogenhagen,
Binding of TFIIIA to derivatives of 5S RNA containing sequence substitutions or deletions defines a minimal TFIIIA binding site.
1992,
Pubmed
,
Xenbase
Christensen,
Sequence-specific binding of the N-terminal three-finger fragment of Xenopus transcription factor IIIA to the internal control region of a 5S RNA gene.
1991,
Pubmed
,
Xenbase
Clemens,
Definition of the binding sites of individual zinc fingers in the transcription factor IIIA-5S RNA gene complex.
1992,
Pubmed
,
Xenbase
Clemens,
Molecular basis for specific recognition of both RNA and DNA by a zinc finger protein.
1993,
Pubmed
,
Xenbase
Cleveland,
Peptide mapping in one dimension by limited proteolysis of sodium dodecyl sulfate-solubilized proteins.
1983,
Pubmed
Darby,
Differential binding of zinc fingers from Xenopus TFIIIA and p43 to 5S RNA and the 5S RNA gene.
1992,
Pubmed
,
Xenbase
Del Río,
High yield purification of active transcription factor IIIA expressed in E. coli.
1991,
Pubmed
,
Xenbase
Fairall,
A new approach to the analysis of DNase I footprinting data and its application to the TFIIIA/5S DNA complex.
1992,
Pubmed
,
Xenbase
Galas,
DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.
1978,
Pubmed
Ginsberg,
Xenopus 5S gene transcription factor, TFIIIA: characterization of a cDNA clone and measurement of RNA levels throughout development.
1984,
Pubmed
,
Xenbase
Gralla,
Rapid "footprinting" on supercoiled DNA.
1985,
Pubmed
Hanas,
Cooperative model for the binding of Xenopus transcription factor A to the 5S RNA gene.
1983,
Pubmed
,
Xenbase
Hanas,
Conformation states of Xenopus transcription factor IIIA.
1989,
Pubmed
,
Xenbase
Hayes,
A protein-protein interaction is essential for stable complex formation on a 5 S RNA gene.
1989,
Pubmed
,
Xenbase
Hayes,
Structure of the TFIIIA-5 S DNA complex.
1992,
Pubmed
,
Xenbase
Honda,
Association of a 5S gene transcription factor with 5S RNA and altered levels of the factor during cell differentiation.
1980,
Pubmed
,
Xenbase
Jue,
Determination of the relative positions of amino acids by partial specific cleavages of end-labeled proteins.
1985,
Pubmed
Klenow,
Selective elimination of the exonuclease activity of the deoxyribonucleic acid polymerase from Escherichia coli B by limited proteolysis.
1970,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lerner,
Chemically synthesized peptides predicted from the nucleotide sequence of the hepatitis B virus genome elicit antibodies reactive with the native envelope protein of Dane particles.
1981,
Pubmed
Liao,
Specific interaction of the first three zinc fingers of TFIIIA with the internal control region of the Xenopus 5 S RNA gene.
1992,
Pubmed
,
Xenbase
Lindsley,
Proteolysis patterns of epitopically labeled yeast DNA topoisomerase II suggest an allosteric transition in the enzyme induced by ATP binding.
1991,
Pubmed
McConkey,
TFIIIA binds with equal affinity to somatic and major oocyte 5S RNA genes.
1988,
Pubmed
,
Xenbase
Miller,
The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. II. The GC-rich region.
1978,
Pubmed
,
Xenbase
Munro,
Use of peptide tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila hsp 70.
1984,
Pubmed
Pelham,
A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.
1980,
Pubmed
,
Xenbase
Peterson,
Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA.
1980,
Pubmed
,
Xenbase
Porter,
Structural studies of immunoglobulins.
1973,
Pubmed
Romaniuk,
A comparison of the solution structures and conformational properties of the somatic and oocyte 5S rRNAs of Xenopus laevis.
1988,
Pubmed
,
Xenbase
Sands,
The carboxyterminal zinc fingers of TFIIIA interact with the tip of helix V of 5S RNA in the 7S ribonucleoprotein particle.
1991,
Pubmed
,
Xenbase
Shang,
Purification of Xenopus transcription factor IIIA and 5 S RNA from 7 S ribonucleoprotein particle by ammonium sulfate precipitation.
1988,
Pubmed
,
Xenbase
Sheshberadaran,
Protein footprinting method for studying antigen-antibody interactions and epitope mapping.
1989,
Pubmed
Smith,
Domains of the positive transcription factor specific for the Xenopus 5S RNA gene.
1984,
Pubmed
,
Xenbase
Theunissen,
RNA and DNA binding zinc fingers in Xenopus TFIIIA.
1992,
Pubmed
,
Xenbase
Wakefield,
Cytoplasmic regulation of 5S RNA genes in nuclear-transplant embryos.
1983,
Pubmed
,
Xenbase
Westhof,
Computer modeling from solution data of spinach chloroplast and of Xenopus laevis somatic and oocyte 5 S rRNAs.
1989,
Pubmed
,
Xenbase
You,
The effects of disrupting 5S RNA helical structures on the binding of Xenopus transcription factor IIIA.
1990,
Pubmed
,
Xenbase
