XB-ART-22264
Gen Comp Endocrinol
1993 Sep 01;913:307-17. doi: 10.1006/gcen.1993.1131.
Show Gene links
Show Anatomy links
Isolation and characterization of two forms of Xenopus prolactin.
???displayArticle.abstract???
Two forms of highly purified prolactin (PRL) were obtained from pituitary glands of Xenopus laevis by extraction of acetone-dried powder with acid acetone and high-performance liquid chromatography on anion exchange, gel filtration, and reverse-phase columns. Purification was monitored by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis employing antiserum against bullfrog PRL. The Xenopus prolactins (xPRL-I and xPRL-II) thus obtained were shown to have similar molecular weights of 23,000 as determined by SDS-PAGE. The isoelectric points of xPRL-I and xPRL-II determined by isoelectric focusing were 5.6 and 5.3, respectively. Both hormones blocked T4-induced shrinkage of Xenopus tadpole tail fin in vitro. The amino acid compositions of the xPRLs resembled that of bullfrog PRL. The partial amino acid sequences of xPRL-I and of xPRL-II showed 78 and 68% homology with the comparable portion of the sequence of bullfrog PRL, respectively. Homology between xPRL-I and xPRL-II was 90%.
???displayArticle.pubmedLink??? 8224774
???displayArticle.link??? Gen Comp Endocrinol
Species referenced: Xenopus laevis
Genes referenced: prl.1 prl.2