Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Vasopressin-dependent control of basolateral Na/H-exchange in highly differentiated A6-cell monolayers.
Guerra L
,
Casavola V
,
Vilella S
,
Verrey F
,
Helmle-Kolb C
,
Murer H
.
???displayArticle.abstract???
We have used a well-differentiated A6-cell preparation (A6-C1) to study cellular location and vasopressin control of Na/H-exchange activity. After cell acidification, cell pHi (measured by BCECF-fluorescence) only recovered by the addition of Na medium to the basolateral cell surface; this pHi recovery was inhibited by dimethylamiloride (2 microM) consistent with basolateral location of Na/H-exchange activity. Addition of vasopressin produced stimulation of Na/H-exchange activity and increased the affinity of the exchanger for Na+. Stimulation of Na/H exchange was mimicked by pharmacological activation of protein kinase A (forskolin, 8-Br-cAMP) and not by pharmacological activation of protein kinase C (TPA). It is concluded that basolaterally located Na/H-exchange in A6-C1 cells is activated by vasopressin.
Alpern,
Cell mechanisms of proximal tubule acidification.
1990, Pubmed
Alpern,
Cell mechanisms of proximal tubule acidification.
1990,
Pubmed
Borgese,
Cloning and expression of a cAMP-activated Na+/H+ exchanger: evidence that the cytoplasmic domain mediates hormonal regulation.
1992,
Pubmed
Boron,
Intracellular pH transients in squid giant axons caused by CO2, NH3, and metabolic inhibitors.
1976,
Pubmed
Casavola,
Na+/H(+)-exchange in A6 cells: polarity and vasopressin regulation.
1992,
Pubmed
Casavola,
Polarized expression of Na+/H+ exchange activity in LLC-PK1/PKE20 cells: II. Hormonal regulation.
1992,
Pubmed
Clark,
Na(+)-H+ exchanger subtypes: a predictive review.
1991,
Pubmed
Harvey,
Intracellular pH controls cell membrane Na+ and K+ conductances and transport in frog skin epithelium.
1988,
Pubmed
Helmle-Kolb,
Parathyroid hormone regulation of Na+/H+ exchange in opossum kidney cells: polarity and mechanisms.
1990,
Pubmed
Krayer-Pawlowska,
Studies on the kinetics of Na+/H+ exchange in OK cells: introduction of a new device for the analysis of polarized transport in cultured epithelia.
1991,
Pubmed
Lang,
Adenosine stimulates sodium transport in kidney A6 epithelia in culture.
1985,
Pubmed
,
Xenbase
Lang,
Complete response to vasopressin requires epithelial organization in A6 cells in culture.
1986,
Pubmed
Ling,
Effects of luminal Na+ on single Na+ channels in A6 cells, a regulatory role for protein kinase C.
1989,
Pubmed
Montrose,
Polarity and kinetics of Na(+)-H+ exchange in cultured opossum kidney cells.
1990,
Pubmed
Mrkic,
Apical and basolateral Na/H exchange in cultured murine proximal tubule cells (MCT): effect of parathyroid hormone (PTH).
1992,
Pubmed
Oberleithner,
Madin-Darby canine kidney cells. II. Aldosterone stimulates Na+/H+ and Cl-/HCO3- exchange.
1990,
Pubmed
Paccolat,
Aldosterone regulation of Na+ transport and Na+-K+-ATPase in A6 cells: role of growth conditions.
1987,
Pubmed
Palmer,
Effects of cell Ca and pH on Na channels from rat cortical collecting tubule.
1987,
Pubmed
Perkins,
Transport properties of toad kidney epithelia in culture.
1981,
Pubmed
,
Xenbase
Preisig,
Basolateral membrane H/HCO3 transport in renal tubules.
1991,
Pubmed
Preston,
Dexamethasone accelerates differentiation of A6 epithelia and increases response to vasopressin.
1988,
Pubmed
,
Xenbase
Rosenberg,
Imaging of filter-grown epithelial cells: MDCK Na(+)-H+ exchanger is basolateral.
1991,
Pubmed
Sariban-Sohraby,
Apical sodium uptake in toad kidney epithelial cell line A6.
1983,
Pubmed
Verrey,
Regulation by aldosterone of Na+,K+-ATPase mRNAs, protein synthesis, and sodium transport in cultured kidney cells.
1987,
Pubmed
,
Xenbase
Verrey,
Regulation of gene expression by aldosterone in tight epithelia.
1990,
Pubmed
Verrey,
Polarized membrane movements in A6 kidney cells are regulated by aldosterone and vasopressin/vasotocin.
1993,
Pubmed
,
Xenbase
Vilella,
Characterization of basolateral Na/H exchange (Na/H-1) in MDCK cells.
1992,
Pubmed
Vilella,
Aldosterone actions on basolateral Na+/H+ exchange in Madin-Darby canine kidney cells.
1992,
Pubmed
Wakabayashi,
The Na+/H+ antiporter cytoplasmic domain mediates growth factor signals and controls "H(+)-sensing".
1992,
Pubmed
