Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Retroviral-type zinc fingers and glycine-rich repeats in a protein encoded by cnjB, a Tetrahymena gene active during meiosis.
Taylor FM
,
Martindale DW
.
???displayArticle.abstract???
We have determined the nucleotide sequence of the cnjB gene from the ciliate Tetrahymena thermophila. This gene is transcriptionally active only during early conjugation, peaking in meiotic prophase. It contains 13 introns, four transcription start points and codes for a putative polypeptide (CnjB) of 1748 amino acids with a calculated molecular weight of 200 kilodaltons and a pl of 7.9. The coding region of cnjB has a low GC content (32% GC) and unusual codon usage. The C-terminal one-third of CnjB consists of three repetitive domains. Introns were absent in this region of cnjB. One of the repetitive domains consists of seven CCHC or retroviral-type zinc fingers, a motif found in one or two copies in retroviral nucleocapsid proteins. This motif has also been found recently in seven copies in the human nucleic-acid binding protein CNBP, in an apparent CNBP homologue in Schizosaccharomyces pombe and in one copy in a Xenopus gene active in early embryos. The other two domains are on either side of the zinc finger domain and contain a repeated glycine-rich motif seen in the heterogeneous nuclear ribonuclear proteins A1 and A2/B1 as well as other proteins. Both CCHC zinc fingers and glycine-rich repeats have been found in proteins with single-stranded nucleic acid-binding activity as well as strand-annealing activity. CnjB is, to our knowledge, the first protein found to contain both types of motifs.
Ball,
Cloning of a grasshopper cDNA coding for a protein homologous to the A1, A2/B1 proteins of mammalian hnRNP.
1991, Pubmed
Ball,
Cloning of a grasshopper cDNA coding for a protein homologous to the A1, A2/B1 proteins of mammalian hnRNP.
1991,
Pubmed
Berg,
Potential metal-binding domains in nucleic acid binding proteins.
1986,
Pubmed
,
Xenbase
Biamonti,
Isolation of an active gene encoding human hnRNP protein A1. Evidence for alternative splicing.
1989,
Pubmed
Bishop,
DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression.
1992,
Pubmed
Brunk,
Characterization of the promoter region of Tetrahymena genes.
1990,
Pubmed
Burd,
Primary structures of the heterogeneous nuclear ribonucleoprotein A2, B1, and C2 proteins: a diversity of RNA binding proteins is generated by small peptide inserts.
1989,
Pubmed
Clark,
Isolation, DNA sequence, and regulation of a Saccharomyces cerevisiae gene that encodes DNA strand transfer protein alpha.
1991,
Pubmed
Cobianchi,
Structure of rodent helix-destabilizing protein revealed by cDNA cloning.
1986,
Pubmed
Csank,
Isoleucyl-tRNA synthetase from the ciliated protozoan Tetrahymena thermophila. DNA sequence, gene regulation, and leucine zipper motifs.
1992,
Pubmed
Csank,
Nuclear pre-mRNA introns: analysis and comparison of intron sequences from Tetrahymena thermophila and other eukaryotes.
1990,
Pubmed
Dykstra,
Cloning and characterization of DST2, the gene for DNA strand transfer protein beta from Saccharomyces cerevisiae.
1991,
Pubmed
Haynes,
pen repeat sequences are GGN clusters and encode a glycine-rich domain in a Drosophila cDNA homologous to the rat helix destabilizing protein.
1987,
Pubmed
Henderson,
Primary structure of the low molecular weight nucleic acid-binding proteins of murine leukemia viruses.
1981,
Pubmed
Henikoff,
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
1984,
Pubmed
Holden,
Nucleotide sequence of the REC1 gene of Ustilago maydis.
1989,
Pubmed
Horii,
Organization of the recA gene of Escherichia coli.
1980,
Pubmed
Horowitz,
Unusual features of transcribed and translated regions of the histone H4 gene family of Tetrahymena thermophila.
1987,
Pubmed
Karpel,
Interactions of retroviral structural proteins with single-stranded nucleic acids.
1987,
Pubmed
Keller,
Glycine-rich cell wall proteins in bean: gene structure and association of the protein with the vascular system.
1988,
Pubmed
Khan,
Recombinant human immunodeficiency virus type 1 nucleocapsid (NCp7) protein unwinds tRNA.
1992,
Pubmed
Kretzschmar,
Molecular cloning of a human prion protein cDNA.
1986,
Pubmed
Kumar,
Mammalian heterogeneous nuclear ribonucleoprotein A1. Nucleic acid binding properties of the COOH-terminal domain.
1990,
Pubmed
Kumar,
Studies of the strand-annealing activity of mammalian hnRNP complex protein A1.
1990,
Pubmed
Martindale,
Conjugation in Tetrahymena thermophila. A temporal analysis of cytological stages.
1982,
Pubmed
Martindale,
Cloning of abundant mRNA species present during conjugation of Tetrahymena thermophila: identification of mRNA species present exclusively during meiosis.
1983,
Pubmed
Martindale,
RNA and protein synthesis during meiotic prophase in Tetrahymena thermophila.
1985,
Pubmed
Martindale,
Tetrahymena conjugation-induced genes: structure and organization in macro- and micronuclei.
1986,
Pubmed
Martindale,
A conjugation-specific gene (cnjC) from Tetrahymena encodes a protein homologous to yeast RNA polymerase subunits (RPB3, RPC40) and similar to a portion of the prokaryotic RNA polymerase alpha subunit (rpoA).
1990,
Pubmed
Martindale,
Multiple introns in a conjugation-specific gene from Tetrahymena thermophila.
1988,
Pubmed
Martindale,
Codon usage in Tetrahymena and other ciliates.
1989,
Pubmed
Maryon,
Involvement of single-stranded tails in homologous recombination of DNA injected into Xenopus laevis oocyte nuclei.
1991,
Pubmed
,
Xenbase
Medved',
The role of fibrinogen alpha C-domains in the fibrin assembly process.
1985,
Pubmed
Mehrel,
Identification of a major keratinocyte cell envelope protein, loricrin.
1990,
Pubmed
Oesch,
A cellular gene encodes scrapie PrP 27-30 protein.
1985,
Pubmed
Prats,
Viral RNA annealing activities of the nucleocapsid protein of Moloney murine leukemia virus are zinc independent.
1991,
Pubmed
Prats,
Small finger protein of avian and murine retroviruses has nucleic acid annealing activity and positions the replication primer tRNA onto genomic RNA.
1988,
Pubmed
Rajavashisth,
Identification of a zinc finger protein that binds to the sterol regulatory element.
1989,
Pubmed
Sancar,
Sequences of the recA gene and protein.
1980,
Pubmed
Sander,
Cloning and characterization of Rrp1, the gene encoding Drosophila strand transferase: carboxy-terminal homology to DNA repair endo/exonucleases.
1991,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Sato,
Localized and inducible expression of Xenopus-posterior (Xpo), a novel gene active in early frog embryos, encoding a protein with a 'CCHC' finger domain.
1991,
Pubmed
,
Xenbase
Shinohara,
Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein.
1992,
Pubmed
South,
Zinc fingers and molecular recognition. Structure and nucleic acid binding studies of an HIV zinc finger-like domain.
1990,
Pubmed
Stargell,
Transcriptional regulation of gene expression in Tetrahymena thermophila.
1990,
Pubmed
Steinert,
Complete amino acid sequence of a mouse epidermal keratin subunit and implications for the structure of intermediate filaments.
1983,
Pubmed
Summers,
High-resolution structure of an HIV zinc fingerlike domain via a new NMR-based distance geometry approach.
1990,
Pubmed
Summers,
Zinc finger motif for single-stranded nucleic acids? Investigations by nuclear magnetic resonance.
1991,
Pubmed
Sun,
Extensive 3'-overhanging, single-stranded DNA associated with the meiosis-specific double-strand breaks at the ARG4 recombination initiation site.
1991,
Pubmed
Sung,
Renaturation of DNA catalysed by yeast DNA repair and recombination protein RAD10.
1992,
Pubmed
Tishkoff,
Molecular and genetic analysis of the gene encoding the Saccharomyces cerevisiae strand exchange protein Sep1.
1991,
Pubmed
Wang,
Complete sequence of the lamprey fibrinogen alpha chain.
1989,
Pubmed
Xu,
A gene encoding a protein with seven zinc finger domains acts on the sexual differentiation pathways of Schizosaccharomyces pombe.
1992,
Pubmed