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Activation of protein kinase C potentiates postsynaptic acetylcholine response at developing neuromuscular synapses.
Fu WM
,
Lin JL
.
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1. Phorbol 12-myristate 13-acetate (TPA, 1 microM) and phorbol 12,13-dibutyrate (PDBu, 2 microM), activators of protein kinase C (PKC), increased the mean amplitude and decay time of the spontaneous synaptic currents of Xenopus nerve-muscle coculture, whereas, 4 alpha-phorbol (2 microM) which is an inactive phorbol analogue had no effect. 2. Staurosporine (0.5 microM) and H-7 (10 microM), inhibitors of PKC, inhibited the potentiation effects of TPA on the spontaneous synaptic currents. 3. Effects of TPA on the postsynaptic acetylcholine (ACh) sensitivity were examined by iontophoresis of ACh to the surface of embryonic muscle cells of 1-day-old Xenopus cultures. TPA increased both the amplitude and decay time of ACh-induced whole-cell currents in isolated myocytes. 4. TPA concentration-dependently increased the mean open time of low-conductance ACh channels but did not affect those of high-conductance ACh channels. PDBu but not 4 alpha-phorbol exhibited similar effects to TPA. Staurosporine and H-7 inhibited the increasing effects of TPA. 5. These results suggest that activation of PKC might be involved in synaptogenesis at developing neuromuscular synapses by the postsynaptic potentiation of ACh sensitivity.
Albuquerque,
A possible involvement of cyclic AMP in the expression of desensitization of the nicotinic acetylcholine receptor. A study with forskolin and its analogs.
1986, Pubmed
Albuquerque,
A possible involvement of cyclic AMP in the expression of desensitization of the nicotinic acetylcholine receptor. A study with forskolin and its analogs.
1986,
Pubmed
Anderson,
Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
1977,
Pubmed
,
Xenbase
Brehm,
Properties of non-junctional acetylcholine receptor channels on innervated muscle of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Brehm,
Acetylcholine receptor channel properties during development of Xenopus muscle cells in culture.
1984,
Pubmed
,
Xenbase
Caratsch,
Postsynaptic effects of the phorbol ester TPA on frog end-plates.
1986,
Pubmed
Caratsch,
Influence of protein kinase C-stimulation by a phorbol ester on neurotransmitter release at frog end-plates.
1988,
Pubmed
Caratsch,
Regulation of acetylcholine receptor function by the phorbol ester TPA in rat skeletal muscle.
1989,
Pubmed
Castagna,
Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.
1982,
Pubmed
Eusebi,
Agents that activate protein kinase C reduce acetylcholine sensitivity in cultured myotubes.
1985,
Pubmed
Eusebi,
Effects of phorbol ester on spontaneous transmitter release at frog neuromuscular junction.
1986,
Pubmed
Eusebi,
Acetylcholine regulation of nicotinic receptor channels through a putative G protein in chick myotubes.
1987,
Pubmed
Evers,
Studies of nerve-muscle interactions in Xenopus cell culture: analysis of early synaptic currents.
1989,
Pubmed
,
Xenbase
Greengard,
Phosphorylated proteins as physiological effectors.
1978,
Pubmed
Haimann,
The phorbol ester, 12-O-tetradecanoyl-phorbol-13-acetate, enhances the evoked quanta release of acetylcholine at the frog neuromuscular junction.
1987,
Pubmed
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Hemmings,
Role of protein phosphorylation in neuronal signal transduction.
1989,
Pubmed
Huganir,
Phosphorylation of the nicotinic acetylcholine receptor by an endogenous tyrosine-specific protein kinase.
1984,
Pubmed
Huganir,
Phosphorylation of the nicotinic acetylcholine receptor regulates its rate of desensitization.
,
Pubmed
Huganir,
cAMP-dependent protein kinase phosphorylates the nicotinic acetylcholine receptor.
1983,
Pubmed
Kennedy,
Experimental approaches to understanding the role of protein phosphorylation in the regulation of neuronal function.
1983,
Pubmed
Kidokoro,
Distribution and density of alpha-bungarotoxin binding sites on innervated and noninnervated Xenopus muscle cells in culture.
1982,
Pubmed
,
Xenbase
Kidokoro,
Early cross-striation formation in twitching Xenopus myocytes in culture.
1988,
Pubmed
,
Xenbase
Kullberg,
Nonjunctional acetylcholine receptor channel open time decreases during development of Xenopus muscle.
1981,
Pubmed
,
Xenbase
Lu,
Calcitonin gene-related peptide potentiates synaptic responses at developing neuromuscular junction.
1993,
Pubmed
,
Xenbase
Middleton,
Desensitization of acetylcholine receptors in rat myotubes is enhanced by agents that elevate intracellular cAMP.
1988,
Pubmed
Middleton,
Forskolin increases the rate of acetylcholine receptor desensitization at rat soleus endplates.
1986,
Pubmed
Mulle,
Calcitonin gene-related peptide enhances the rate of desensitization of the nicotinic acetylcholine receptor in cultured mouse muscle cells.
1988,
Pubmed
Murphy,
Effects of diacylglycerol and phorbol ester on acetylcholine release and action at the neuromuscular junction in mice.
1987,
Pubmed
Nishizuka,
Turnover of inositol phospholipids and signal transduction.
1984,
Pubmed
Nishizuka,
The molecular heterogeneity of protein kinase C and its implications for cellular regulation.
1988,
Pubmed
Nishizuka,
Studies and perspectives of protein kinase C.
1986,
Pubmed
Owens,
In vivo development of nicotinic acetylcholine receptor channels in Xenopus myotomal muscle.
1989,
Pubmed
,
Xenbase
Rohrbough,
Changes in kinetics of acetylcholine receptor channels after initial expression in Xenopus myocyte culture.
1990,
Pubmed
,
Xenbase
Sanes,
In vitro analysis of position- and lineage-dependent selectivity in the formation of neuromuscular synapses.
1989,
Pubmed
,
Xenbase
Shapira,
Activation of protein kinase C augments evoked transmitter release.
,
Pubmed
Shearman,
Modulation of ion channel activity: a key function of the protein kinase C enzyme family.
1989,
Pubmed
Spitzer,
The development of the action potential mechanism of amphibian neurons isolated in culture.
1976,
Pubmed
,
Xenbase
Xie,
Initial events in the formation of neuromuscular synapse: rapid induction of acetylcholine release from embryonic neuron.
1986,
Pubmed
,
Xenbase