Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Methylphosphate cap structure increases the stability of 7SK, B2 and U6 small RNAs in Xenopus oocytes.
Shumyatsky G
,
Wright D
,
Reddy R
.
???displayArticle.abstract???
We studied the role of the methylphosphate cap structure in the stability and nucleocytoplasmic transport by microinjecting U6, 7SK and B2 RNAs into the Xenopus oocytes. In every case, the methylphosphate capped RNAs were 3 to 9 times more stable than the uncapped RNAs. When a methylphosphate cap structure was placed on human H1 RNA which is normally not capped, its stability was improved 2-7 fold. These data show that the methylphosphate cap enhances the stability of 7SK, B2, H1 and U6 RNAs. The methylphosphate-capped 7SK RNA was transported into the nucleus from cytoplasm, but remained in the nucleus when injected into the nucleus; in this respect, 7SK RNA exhibited properties previously shown for U6 RNA. Both U6 and 7SK RNAs with ppp on their 5' ends were transported from cytoplasm to the nucleus suggesting that the methylphosphate cap structure is not required for transport of these RNAs across the nuclear membrane.
Adeniyi-Jones,
Transcription, processing and nuclear transport of a B1 Alu RNA species complementary to an intron of the murine alpha-fetoprotein gene.
, Pubmed,
Xenbase
Adeniyi-Jones,
Transcription, processing and nuclear transport of a B1 Alu RNA species complementary to an intron of the murine alpha-fetoprotein gene.
,
Pubmed
,
Xenbase
Baer,
Structure and transcription of a human gene for H1 RNA, the RNA component of human RNase P.
1990,
Pubmed
Banerjee,
5'-terminal cap structure in eucaryotic messenger ribonucleic acids.
1980,
Pubmed
Baserga,
Distinct molecular signals for nuclear import of the nucleolar snRNA, U3.
1992,
Pubmed
,
Xenbase
Das,
Upstream regulatory elements are necessary and sufficient for transcription of a U6 RNA gene by RNA polymerase III.
1988,
Pubmed
,
Xenbase
De Robertis,
Intracellular transport of microinjected 5S and small nuclear RNAs.
1982,
Pubmed
,
Xenbase
Fischer,
An essential signaling role for the m3G cap in the transport of U1 snRNP to the nucleus.
1990,
Pubmed
,
Xenbase
Fischer,
Diversity in the signals required for nuclear accumulation of U snRNPs and variety in the pathways of nuclear transport.
1991,
Pubmed
,
Xenbase
Gupta,
Characterization of U6 small nuclear RNA cap-specific antibodies. Identification of gamma-monomethyl-GTP cap structure in 7SK and several other human small RNAs.
1990,
Pubmed
Guthrie,
Messenger RNA splicing in yeast: clues to why the spliceosome is a ribonucleoprotein.
1991,
Pubmed
Hamm,
An abundant U6 snRNP found in germ cells and embryos of Xenopus laevis.
1989,
Pubmed
,
Xenbase
Hamm,
The trimethylguanosine cap structure of U1 snRNA is a component of a bipartite nuclear targeting signal.
1990,
Pubmed
,
Xenbase
Hamm,
Monomethylated cap structures facilitate RNA export from the nucleus.
1990,
Pubmed
,
Xenbase
Kiss,
Alteration of the RNA polymerase specificity of U3 snRNA genes during evolution and in vitro.
1991,
Pubmed
Kleinschmidt,
RNA processing and ribonucleoprotein assembly studied in vivo by RNA transfection.
1990,
Pubmed
Kramerov,
The sequences homologous to major interspersed repeats B1 and B2 of mouse genome are present in mRNA and small cytoplasmic poly(A) + RNA.
1982,
Pubmed
Kramerov,
Biosynthesis and cytoplasmic distribution of small poly(A)-containing B2 RNA.
1985,
Pubmed
Krayev,
Ubiquitous transposon-like repeats B1 and B2 of the mouse genome: B2 sequencing.
1982,
Pubmed
Lerner,
Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus.
1979,
Pubmed
Maraia,
Pathway of B1-Alu expression in microinjected oocytes: Xenopus laevis proteins associated with nuclear precursor and processed cytoplasmic RNAs.
1988,
Pubmed
,
Xenbase
Michaud,
Multiple pathways in nuclear transport: the import of U2 snRNP occurs by a novel kinetic pathway.
1991,
Pubmed
,
Xenbase
Michaud,
Microinjected U snRNAs are imported to oocyte nuclei via the nuclear pore complex by three distinguishable targeting pathways.
1992,
Pubmed
,
Xenbase
Murphy,
The in vitro transcription of the 7SK RNA gene by RNA polymerase III is dependent only on the presence of an upstream promoter.
1987,
Pubmed
Reddy,
Methylated cap structures in eukaryotic RNAs: structure, synthesis and functions.
1992,
Pubmed
Reddy,
Characterization and subcellular localization of 7-8 S RNAs of Novikoff hepatoma.
1981,
Pubmed
Reddy,
Detection of a nucleolar 7-2 ribonucleoprotein and a cytoplasmic 8-2 ribonucleoprotein with autoantibodies from patients with scleroderma.
1983,
Pubmed
Shatkin,
Capping of eucaryotic mRNAs.
1976,
Pubmed
Shatkin,
mRNA cap binding proteins: essential factors for initiating translation.
1985,
Pubmed
Shimba,
Cap structure of U3 small nucleolar RNA in animal and plant cells is different. gamma-Monomethyl phosphate cap structure in plant RNA.
1992,
Pubmed
Shumyatsky,
B2 RNA and 7SK RNA, RNA polymerase III transcripts, have a cap-like structure at their 5' end.
1990,
Pubmed
Singh,
Gamma-monomethyl phosphate: a cap structure in spliceosomal U6 small nuclear RNA.
1989,
Pubmed
Tazi,
Mammalian U6 small nuclear RNA undergoes 3' end modifications within the spliceosome.
1993,
Pubmed
Terns,
3'-end-dependent formation of U6 small nuclear ribonucleoprotein particles in Xenopus laevis oocyte nuclei.
1992,
Pubmed
,
Xenbase
Vankan,
Domains of U4 and U6 snRNAs required for snRNP assembly and splicing complementation in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
