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Chromosoma
1993 Nov 01;1029:599-611. doi: 10.1007/bf00352307.
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The RNA polymerase I-specific transcription initiation factor UBF is associated with transcriptionally active and inactive ribosomal genes.
Zatsepina OV
,
Voit R
,
Grummt I
,
Spring H
,
Semenov MV
,
Trendelenburg MF
.
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We have characterized an anti-NOR (nucleolar organizer region) serum (P419) from a patient with rheumatoid arthritis and show that it contains antibodies directed against the RNA polymerase I-specific transcription initiation factor UBF. This serum reacts with UBF from a variety of vertebrate cells as revealed both by immunoblotting and by indirect immunofluorescence. We have used the P419 serum to study the intracellular localization of this transcription factor at the light and electron microscopic level. In interphase cells, UBF exhibits a pronounced punctate pattern and is found to be associated with necklace-like structures, which appear to reflect the transcriptionally active state of the nucleolus. Inhibition of rRNA synthetic activity caused either by nutritional starvation or by actinomycin D treatment resulted in a marked decrease in the number and in a significant increase in the size of UBF-positive granules. Under all experimental conditions applied, UBF was exclusively found within the nucleolus and was not released into the nucleoplasm or cytoplasm. During mitosis, UBF was found to be concentrated at the chromosomal NOR indicating that a significant quantity, if not all, of this factor remains bound to the ribosomal transcription units. From this we conclude that UBF is associated both with transcriptionally active and inactive rRNA genes and, therefore, changes in the intracellular localization of UBF are very likely not involved in rDNA transcription regulation.
Bachvarov,
The RNA polymerase I transcription factor xUBF contains 5 tandemly repeated HMG homology boxes.
1991, Pubmed,
Xenbase
Bachvarov,
The RNA polymerase I transcription factor xUBF contains 5 tandemly repeated HMG homology boxes.
1991,
Pubmed
,
Xenbase
Buttgereit,
Growth-dependent regulation of rRNA synthesis is mediated by a transcription initiation factor (TIF-IA).
1985,
Pubmed
Chan,
Human autoantibody to RNA polymerase I transcription factor hUBF. Molecular identity of nucleolus organizer region autoantigen NOR-90 and ribosomal RNA transcription upstream binding factor.
1991,
Pubmed
Goenechea,
Immunostaining of nucleolus organizers in mammalian cells by a human autoantibody against the polymerase I transcription factor UBF.
1992,
Pubmed
Grummt,
Amino acid starvation affects the initiation frequency of nucleolar RNA polymerase.
1976,
Pubmed
Guldner,
Scl 70 autoantibodies from scleroderma patients recognize a 95 kDa protein identified as DNA topoisomerase I.
1986,
Pubmed
Haaf,
Quantitative determination of rDNA transcription units in vertebrate cells.
1991,
Pubmed
Hernandez-Verdun,
The nucleolus today.
1991,
Pubmed
Hisatake,
Cloning and structural analysis of cDNA and the gene for mouse transcription factor UBF.
1991,
Pubmed
Howell,
Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method.
1980,
Pubmed
Hozák,
An electron microscopic study of nucleolus-organizing regions at some stages of the cell cycle (G0 period, G2 period, mitosis).
1986,
Pubmed
Hsu,
Distribution of 18+28S ribosomal genes in mammalian genomes.
1975,
Pubmed
,
Xenbase
Jantzen,
Nucleolar transcription factor hUBF contains a DNA-binding motif with homology to HMG proteins.
1990,
Pubmed
Jordan,
Interpreting nucleolar structure: where are the transcribing genes?
1991,
Pubmed
Jordan,
The quantitative relationship of the fibrillar centres and other nucleolar components to changes in growth conditions, serum deprivation and low doses of actinomycin D in cultured diploid human fibroblasts (strain MRC-5).
1981,
Pubmed
Lischwe,
Localization of phosphoprotein C23 to nucleolar structures and to the nucleolus organizer regions.
1981,
Pubmed
McStay,
xUBF and Rib 1 are both required for formation of a stable polymerase I promoter complex in X. laevis.
1991,
Pubmed
,
Xenbase
Mishima,
The mechanism of decrease in nucleolar RNA synthesis by protein synthesis inhibition.
1979,
Pubmed
O'Mahony,
Differential phosphorylation and localization of the transcription factor UBF in vivo in response to serum deprivation. In vitro dephosphorylation of UBF reduces its transactivation properties.
1992,
Pubmed
O'Mahony,
Identification of two forms of the RNA polymerase I transcription factor UBF.
1991,
Pubmed
Reimer,
Human autoantibodies: probes for nucleolus structure and function.
1987,
Pubmed
Rendón,
Characterization and immunolocalization of a nucleolar antigen with anti-NOR serum in HeLa cells.
1992,
Pubmed
Rodrigo,
Characterization and immunolocalization of RNA polymerase I transcription factor UBF with anti-NOR serum in protozoa, higher plant and vertebrate cells.
1992,
Pubmed
Rodriguez-Sanchez,
Anti-NOR 90. A new autoantibody in scleroderma that recognizes a 90-kDa component of the nucleolus-organizing region of chromatin.
1987,
Pubmed
Rose,
Association of DNA topoisomerase I and RNA polymerase I: a possible role for topoisomerase I in ribosomal gene transcription.
1988,
Pubmed
Roussel,
Localization of the RNA polymerase I transcription factor hUBF during the cell cycle.
1993,
Pubmed
SHAW,
THE CHROMOSOMES OF THE TASMANIAN RAT-KANGAROO (POTOROUS TRIDACTYLIS APICALIS).
1964,
Pubmed
Scheer,
Functional and dynamic aspects of the mammalian nucleolus.
1990,
Pubmed
Scheer,
Drug-induced dispersal of transcribed rRNA genes and transcriptional products: immunolocalization and silver staining of different nucleolar components in rat cells treated with 5,6-dichloro-beta-D-ribofuranosylbenzimidazole.
1984,
Pubmed
Scheer,
Localization of RNA polymerase I in interphase cells and mitotic chromosomes by light and electron microscopic immunocytochemistry.
1984,
Pubmed
Schnapp,
A growth-dependent transcription initiation factor (TIF-IA) interacting with RNA polymerase I regulates mouse ribosomal RNA synthesis.
1990,
Pubmed
Schnapp,
Transcription complex formation at the mouse rDNA promoter involves the stepwise association of four transcription factors and RNA polymerase I.
1991,
Pubmed
Spring,
DNA-fluorescence of mammalian intra-nucleolar chromatin detected by confocal laser scanning microscopy (CLSM).
1988,
Pubmed
Tan,
Autoantibodies in pathology and cell biology.
1991,
Pubmed
Tan,
Antinuclear antibodies: diagnostic markers for autoimmune diseases and probes for cell biology.
1989,
Pubmed
Tower,
Transcription of mouse rDNA is regulated by an activated subform of RNA polymerase I.
1987,
Pubmed
Trendelenburg,
Direct visualization of intranuclear lampbrush chromosome gene domains using videomicroscopy.
1988,
Pubmed
,
Xenbase
Voit,
The nucleolar transcription factor mUBF is phosphorylated by casein kinase II in the C-terminal hyperacidic tail which is essential for transactivation.
1992,
Pubmed
Zatsepina,
Quantitative ultrastructural study of nucleolus-organizing regions at some stages of the cell cycle (G0 period, G2 period, mitosis).
1988,
Pubmed
