FEBS Lett 1993 Nov 22;3343:257-60.

Selectivity of phospholipase C isozymes in growth factor signaling.

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Xenopus laevis oocytes were injected with mRNA extracted from growth factor-responsive CCL39, Chinese hamster lung fibroblasts. The expression of functional growth factor receptors on the oocytes was demonstrated by growth factor-induced 45Ca2+ efflux. To determine the isozyme(s) of phospholipase C (PLC) coupled to growth factor receptors, growth factor-induced 45Ca2+ efflux were measured following coinjection of mRNA from CCL39 cells with PLC antibodies. PLC-gamma 1 antibody did not lead to loss of 45Ca2+ efflux induced by thrombin but resulted in loss of that induced by platelet-derived growth factor (PDGF). In contrast, PLC-delta 1 antibody did not block PDGF-induced 45Ca2+ efflux but led to inhibition of thrombin-induced 45Ca2+ efflux. PLC-beta 1 antibody did not affect Ca2+ efflux by the treatment of either thrombin or PDGF. These results suggest that these growth factor receptors are coupled to specific effectors, i.e. thrombin receptor to PLC-delta 1 and PDGF receptor to PLC-gamma 1.

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Species referenced: Xenopus laevis
Genes referenced: pdgfa