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G protein-mediated inhibition of phosphoinositide metabolism evoked by metabotropic glutamate receptors in frog oocytes.
Nakamura K
,
Nukada T
,
Haga T
,
Sugiyama H
.
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1. Metabotropic glutamate receptor subtype 1 (mGluR1), when expressed in Xenopus oocytes, activates phosphoinositide-specific phospholipase C (PLC) in a G protein-dependent manner. This reaction results in the activation of chloride channels in the oocytes, and can be monitored electrophysiologically. We expressed different G protein alpha-subunits together with mGluR1 in oocytes, and examined the effects of these G protein subunits on the PLC-mediated reaction. 2. The expression of the alpha-subunit of GL2, a bovine version of G11, which is a member of the Gq subgroup, potentiated the mGluR1-evoked reaction, whereas the alpha-subunit of GL1, a bovine G14, which is also a member of the Gq subgroup, strongly suppressed it. The expression of Gs alpha also suppressed this reaction. 3. We then expressed G beta 1 gamma 2-subunits in addition to the G alpha-subunits, and examined the mGluR1-evoked reactions. Both the potentiation and suppression by GL2 alpha and GL1 alpha, respectively, were more pronounced in the presence of the G beta 1 gamma 2-subunits. In contrast, the suppression by Gs alpha was completely reversed by G beta 1 gamma 2. 4. The direct activation of G proteins by the intracellular injection of either fluoride ions or guanosine-5'-O-(3-thiotriphosphate) (GTP gamma S) causes similar PLC-mediated reactions. The expression of GL2 alpha, GL1 alpha or Gs alpha caused potentiation, suppression and no change, respectively, on the fluoride- (or GTP gamma S-) evoked reactions.(ABSTRACT TRUNCATED AT 250 WORDS)
Camps,
Isozyme-selective stimulation of phospholipase C-beta 2 by G protein beta gamma-subunits.
1992, Pubmed
Camps,
Isozyme-selective stimulation of phospholipase C-beta 2 by G protein beta gamma-subunits.
1992,
Pubmed
Dascal,
Involvement of a GTP-binding protein in mediation of serotonin and acetylcholine responses in Xenopus oocytes injected with rat brain messenger RNA.
1986,
Pubmed
,
Xenbase
Enjalbert,
Dual mechanisms of inhibition by dopamine of basal and thyrotropin-releasing hormone-stimulated inositol phosphate production in anterior pituitary cells. Evidence for an inhibition not mediated by voltage-dependent Ca2+ channels.
1990,
Pubmed
Gautam,
A G protein gamma subunit shares homology with ras proteins.
1989,
Pubmed
Ito,
Roles of protein kinases in neurotransmitter responses in Xenopus oocytes injected with rat brain mRNA.
1988,
Pubmed
,
Xenbase
Kaneko,
GTP-binding proteins Gi and Go transplanted onto Xenopus oocyte by rat brain messenger RNA.
1987,
Pubmed
,
Xenbase
Kato,
Phorbol ester inhibition of current responses and simultaneous protein phosphorylation in Xenopus oocyte injected with brain mRNA.
1988,
Pubmed
,
Xenbase
Katz,
Subunits beta gamma of heterotrimeric G protein activate beta 2 isoform of phospholipase C.
1992,
Pubmed
Lee,
Members of the Gq alpha subunit gene family activate phospholipase C beta isozymes.
1992,
Pubmed
Linden,
Receptors that inhibit phosphoinositide breakdown.
1989,
Pubmed
Masu,
Sequence and expression of a metabotropic glutamate receptor.
1991,
Pubmed
,
Xenbase
Minakami,
Postnatal development of mRNA specific for a metabotropic glutamate receptor in the rat brain.
1992,
Pubmed
,
Xenbase
Nakamura,
Identification of two novel GTP-binding protein alpha-subunits that lack apparent ADP-ribosylation sites for pertussis toxin.
1991,
Pubmed
Nukada,
Primary structure of the alpha-subunit of bovine adenylate cyclase-stimulating G-protein deduced from the cDNA sequence.
1986,
Pubmed
Simon,
Diversity of G proteins in signal transduction.
1991,
Pubmed
Smrcka,
Regulation of polyphosphoinositide-specific phospholipase C activity by purified Gq.
1991,
Pubmed
Sugimoto,
Primary structure of the beta-subunit of bovine transducin deduced from the cDNA sequence.
1985,
Pubmed
Sugiyama,
A new type of glutamate receptor linked to inositol phospholipid metabolism.
,
Pubmed
,
Xenbase
Wilkie,
Characterization of G-protein alpha subunits in the Gq class: expression in murine tissues and in stromal and hematopoietic cell lines.
1991,
Pubmed
Wu,
Activation of phospholipase C by the alpha subunits of the Gq and G11 proteins in transfected Cos-7 cells.
1992,
Pubmed