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Expression of a rat renal sodium-dependent dicarboxylate transporter in Xenopus oocytes.
Steffgen J
,
Kienle S
,
Scheyerl F
,
Franz HE
.
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Microinjection of mRNA isolated from rat kidney cortex into Xenopus laevis oocytes resulted in the expression of a Na(+)-dependent dicarboxylate transporter, as detected by uptake measurements with [14C]succinate as substrate. The expressed transporter showed an S-shaped Na(+)-dependence with half-maximal activation at 19-21 mM Na+ and a Hill coefficient between 2 and 3. Endogenous succinate uptake was not Na(+)-dependent. Na(+)-stimulated succinate uptake in mRNA-injected oocytes exhibited a maximum at pH 7.5, whereas endogenous Na(+)-independent transporter was fastest at pH 8.5. The expressed dicarboxylate transporter also differed from the endogenous transporter in its sensitivity to citrate as well as dicarboxylates in trans and cis configurations. The expressed transporter resembled the renal basolateral transporter, especially with respect to affinity for succinate (Km 28 microM), activation by Na+, pH-dependence and substrate specificity. After injection of size-fractionated mRNA, succinate uptake was expressed by mRNA of 2-3 kb. Our results suggest expression of the basolateral Na(+)-dependent dicarboxylate transporter after injection of mRNA from rat kidney into Xenopus oocytes.
Burckhardt,
Proton transport mechanism in the cell membrane of Xenopus laevis oocytes.
1992, Pubmed,
Xenbase
Burckhardt,
Proton transport mechanism in the cell membrane of Xenopus laevis oocytes.
1992,
Pubmed
,
Xenbase
Burckhardt,
Organic anion transport across the contraluminal membrane--dependence on sodium.
1989,
Pubmed
Burckhardt,
Sodium-dependent dicarboxylate transport in rat renal basolateral membrane vesicles.
1984,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Coady,
Expression of mammalian renal transporters in Xenopus laevis oocytes.
1990,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Eckard,
Sodium-dependent and sodium-independent phosphate uptake by full-grown, prophase-arrested oocytes of Xenopus laevis before and after progesterone-induced maturation.
1987,
Pubmed
,
Xenbase
Fukuhara,
Sodium-dependent succinate transport in renal outer cortical brush border membrane vesicles.
1983,
Pubmed
Hediger,
Expression cloning and cDNA sequencing of the Na+/glucose co-transporter.
,
Pubmed
,
Xenbase
Heikkila,
Use of Xenopus oocytes to study the expression of cloned genes and translation of mRNA.
1989,
Pubmed
,
Xenbase
Jung,
Sodium-alanine cotransport in oocytes of Xenopus laevis: correlation of alanine and sodium fluxes with potential and current changes.
1984,
Pubmed
,
Xenbase
Jørgensen,
Citrate uptake by basolateral and luminal membrane vesicles from rabbit kidney cortex.
1983,
Pubmed
Said,
Uptake of biotin by native Xenopus laevis oocytes.
1990,
Pubmed
,
Xenbase
Sheridan,
Reabsorption of dicarboxylic acids from the proximal convolution of rat kidney.
1983,
Pubmed
Simpson,
Citrate excretion: a window on renal metabolism.
1983,
Pubmed
Steffgen,
Endogenous L-glutamate transport in oocytes of Xenopus laevis.
1991,
Pubmed
,
Xenbase
Ullrich,
Secretion and contraluminal uptake of dicarboxylic acids in the proximal convolution of rat kidney.
1984,
Pubmed
Ullrich,
Contraluminal organic anion and cation transport in the proximal renal tubule: V. Interaction with sulfamoyl- and phenoxy diuretics, and with beta-lactam antibiotics.
1989,
Pubmed
Weber,
Comparison of a Na+/D-glucose cotransporter from rat intestine expressed in oocytes of Xenopus laevis with the endogenous cotransporter.
1991,
Pubmed
,
Xenbase
Werner,
Expression of renal transport systems for inorganic phosphate and sulfate in Xenopus laevis oocytes.
1990,
Pubmed
,
Xenbase
Wright,
Succinate and citrate transport in renal basolateral and brush-border membranes.
1987,
Pubmed
Wright,
Effect of pH on the transport of Krebs cycle intermediates in renal brush border membranes.
1982,
Pubmed
Wright,
Transport of carboxylic acids by renal membrane vesicles.
1985,
Pubmed