Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Constitutive expression of a saturable transport system for non-esterified fatty acids in Xenopus laevis oocytes.
Zhou SL
,
Stump D
,
Isola L
,
Berk PD
.
???displayArticle.abstract???
In the presence of 150 microM BSA, uptake of [3H]oleate by Xenopus laevis oocytes was a saturable function of the unbound oleate concentration (Vmax. 110 +/- 4 pmol/h per oocyte; Km 193 +/- 11 nM unbound oleate). Oleate uptake was three orders of magnitude faster than that of another test substance, [35S]bromosulphophthalein, and was competitively inhibited by 55 nM unbound palmitate (Vmax. 111 +/- 14 pmol/h per oocyte; Km 424 +/- 63 nM unbound oleate) (P < 0.01). Oleate uptake was also inhibited by antibodies to a 43 kDa rat liver plasma-membrane fatty acid-binding protein, a putative transporter of long-chain fatty acids in mammalian cells; uptake of the medium-chain fatty acid [14C]octanoate was unaffected. Immunofluorescence and immunoblotting demonstrated that the antiserum reacted with a single 43 kDa protein on the oocyte surface. Hence a protein related to the mammalian plasma-membrane fatty acid-binding protein may play a role in saturable uptake of long-chain fatty acids by Xenopus oocytes.
Abumrad,
Mechanism of long chain fatty acid permeation in the isolated adipocyte.
1981, Pubmed
Abumrad,
Mechanism of long chain fatty acid permeation in the isolated adipocyte.
1981,
Pubmed
Abumrad,
Permeation of long-chain fatty acid into adipocytes. Kinetics, specificity, and evidence for involvement of a membrane protein.
1984,
Pubmed
Ananthanarayanan,
Identification of the hepatocyte Na+-dependent bile acid transport protein using monoclonal antibodies.
1988,
Pubmed
Berk,
Plasma membrane fatty acid-binding protein and mitochondrial glutamic-oxaloacetic transaminase of rat liver are related.
1990,
Pubmed
Black,
Purification and characterization of an outer membrane-bound protein involved in long-chain fatty acid transport in Escherichia coli.
1987,
Pubmed
Boyer,
Phylogenic and ontogenic expression of hepatocellular bile acid transport.
1993,
Pubmed
,
Xenbase
Fletcher,
Analysis of macromolecule--ligand binding by determination of stepwise equilibrium constants.
1970,
Pubmed
Fletcher,
Computer analysis of drug-protein binding data.
1973,
Pubmed
Fricker,
Identification of a single sinusoidal bile salt uptake system in skate liver.
1987,
Pubmed
Glatz,
Cellular fatty acid-binding proteins: current concepts and future directions.
,
Pubmed
Hagenbuch,
Functional expression cloning and characterization of the hepatocyte Na+/bile acid cotransport system.
1991,
Pubmed
,
Xenbase
Harmon,
Labeling of adipocyte membranes by sulfo-N-succinimidyl derivatives of long-chain fatty acids: inhibition of fatty acid transport.
1991,
Pubmed
Hediger,
Expression cloning and cDNA sequencing of the Na+/glucose co-transporter.
,
Pubmed
,
Xenbase
Janicot,
Activation of glucose uptake by insulin and insulin-like growth factor I in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Kuhl,
Uptake of long-chain fatty acid methyl esters by mammalian cells.
1970,
Pubmed
Kurisu,
Preparation of [35S]sulfobromophthalein of high specific activity.
1989,
Pubmed
Nunn,
Transport of long-chain fatty acids in Escherichia coli. Evidence for role of fadL gene product as long-chain fatty acid receptor.
1986,
Pubmed
Potter,
Isolation and partial characterization of plasma membrane fatty acid binding proteins from myocardium and adipose tissue and their relationship to analogous proteins in liver and gut.
1987,
Pubmed
Said,
Nucleotide sequencing and expression of the fadL gene involved in long-chain fatty acid transport in Escherichia coli.
1988,
Pubmed
Schwieterman,
Uptake of oleate by isolated rat adipocytes is mediated by a 40-kDa plasma membrane fatty acid binding protein closely related to that in liver and gut.
1988,
Pubmed
Smith,
Taurocholate uptake by isolated skate hepatocytes: effect of albumin.
1987,
Pubmed
Sorrentino,
From albumin to the cytoplasm: the hepatic uptake of organic anions.
1990,
Pubmed
Sorrentino,
Sex differences in hepatic fatty acid uptake reflect a greater affinity of the transport system in females.
1992,
Pubmed
Sorrentino,
Oleate uptake by cardiac myocytes is carrier mediated and involves a 40-kD plasma membrane fatty acid binding protein similar to that in liver, adipose tissue, and gut.
1988,
Pubmed
Spector,
Analysis of long-chain free fatty acid binding to bovine serum albumin by determination of stepwise equilibrium constants.
1971,
Pubmed
Stremmel,
Isolation and partial characterization of a fatty acid binding protein in rat liver plasma membranes.
1985,
Pubmed
Stremmel,
Hepatocellular influx of [14C]oleate reflects membrane transport rather than intracellular metabolism or binding.
1986,
Pubmed
Stremmel,
Hepatocellular uptake of oleate is energy dependent, sodium linked, and inhibited by an antibody to a hepatocyte plasma membrane fatty acid binding protein.
1986,
Pubmed
Stremmel,
Selective inhibition of long-chain fatty acid uptake in short-term cultured rat hepatocytes by an antibody to the rat liver plasma membrane fatty acid-binding protein.
1986,
Pubmed
Stremmel,
Uptake of fatty acids by jejunal mucosal cells is mediated by a fatty acid binding membrane protein.
1988,
Pubmed
Stump,
Characteristics of oleate binding to liver plasma membranes and its uptake by isolated hepatocytes.
1992,
Pubmed
Swick,
Promoter-cDNA-directed heterologous protein expression in Xenopus laevis oocytes.
1992,
Pubmed
,
Xenbase
Tarnuzzer,
Expression of the mammalian system A neutral amino acid transporter in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Trigatti,
Photoaffinity labeling and fatty acid permeation in 3T3-L1 adipocytes.
1991,
Pubmed
Van 't Klooster,
Rapid method for the determination and quantification of bromosulphophthalein and metabolites in cultured hepatocytes, culture media and bile by high-performance liquid chromatography.
1988,
Pubmed
Wieland,
Identity of hepatic membrane transport systems for bile salts, phalloidin, and antamanide by photoaffinity labeling.
1984,
Pubmed
Wosilait,
A method of computing drug distribution in plasma using stepwise association constants: clofibrate acid as an illustrative example.
1976,
Pubmed
Zhou,
Adipocyte differentiation of 3T3-L1 cells involves augmented expression of a 43-kDa plasma membrane fatty acid-binding protein.
1992,
Pubmed
von Dippe,
Expression of the bile acid transport protein during liver development and in hepatoma cells.
1990,
Pubmed