Mutat Res 1994 May 01;3071:223-8. doi: 10.1016/0027-5107(94)90295-x.

Analysis of benzo[a]pyrene induced mutations by the use of Restriction-Site Mutation assays in aquatic species.

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Larval and toadlet stages of the clawed toad Xenopus laevis were exposed to benzo[a]pyrene in aquatic media. Mutations were analysed in a variety of restriction enzyme recognition sequences of the adult alpha 1-globin gene. Mutations were detected in the BsiLi recognition site (CCTGG) at early sampling times (6 h) in larval stages and at 8-days sampling time in post-metamorphosis toadlets. The predominant mutation detected was a G-->T base transversion at the 5th base of the CCTGG sequence. The data presented indicates that the Restriction-Site Mutation methodology has considerable potential for development as a technique for monitoring the genotoxic potential of water-borne toxins.

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