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Nonhomologous DNA end joining of synthetic hairpin substrates in Xenopus laevis egg extracts.
Beyert N
,
Reichenberger S
,
Peters M
,
Hartung M
,
Göttlich B
,
Goedecke W
,
Vielmetter W
,
Pfeiffer P
.
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Processes of DNA end joining are assumed to play a major role in the elimination of DNA double-strand breaks (DSB) in higher eucaryotic cells. Linear plasmid molecules terminated by nonhomologous restriction ends are the typical substrates used in the analysis of joining mechanisms. However, due to their limited structural variability, DSB ends generated by restriction cleavage cover probably only part of the total spectrum of naturally occurring DSB termini. We therefore devised novel DNA substrates consisting of synthetic hairpin-shaped oligonucleotides which permit the construction of blunt ends and 5'- or 3'-protruding single-strands (PSS) of arbitrary sequence and length. These substrates were tested in extracts of Xenopus laevis eggs known to efficiently join linear plasmids bearing nonhomologous restriction termini (Pfeiffer and Vielmetter, 1988). Sequences of hairpin junctions indicate that the short hairpins are joined by the same mechanisms as the plasmid substrates. However, the bimolecular DNA end joining reaction was only detectable when both hairpin partners had a minimal duplex stem length of 27bp and their PSS-tails did not exceed 10nt.
Bennett,
Lethality induced by a single site-specific double-strand break in a dispensable yeast plasmid.
1993, Pubmed
Bennett,
Lethality induced by a single site-specific double-strand break in a dispensable yeast plasmid.
1993,
Pubmed
Bradley,
X-ray induced DNA double strand break production and repair in mammalian cells as measured by neutral filter elution.
1979,
Pubmed
Derbyshire,
Nonhomologous recombination in human cells.
1994,
Pubmed
Fairman,
Multiple components are involved in the efficient joining of double stranded DNA breaks in human cell extracts.
1992,
Pubmed
Folger,
Patterns of integration of DNA microinjected into cultured mammalian cells: evidence for homologous recombination between injected plasmid DNA molecules.
1982,
Pubmed
Frankenberg,
Interpretation of the shape of survival curves in terms of induction and repair/misrepair of DNA double-strand breaks.
1984,
Pubmed
Friedberg,
Deoxyribonucleic acid repair in the yeast Saccharomyces cerevisiae.
1988,
Pubmed
Jeggo,
Studies on mammalian mutants defective in rejoining double-strand breaks in DNA.
1990,
Pubmed
Kramer,
Two different types of double-strand breaks in Saccharomyces cerevisiae are repaired by similar RAD52-independent, nonhomologous recombination events.
1994,
Pubmed
Lehman,
Homologous and illegitimate recombination in developing Xenopus oocytes and eggs.
1993,
Pubmed
,
Xenbase
Munz,
End-joining of DNA fragments in adenovirus transfection of human cells.
1991,
Pubmed
Nicolás,
Characterization of DNA end joining in a mammalian cell nuclear extract: junction formation is accompanied by nucleotide loss, which is limited and uniform but not site specific.
1994,
Pubmed
North,
The rejoining of double-strand breaks in DNA by human cell extracts.
1990,
Pubmed
Pfeiffer,
Mechanisms of overlap formation in nonhomologous DNA end joining.
1994,
Pubmed
,
Xenbase
Pfeiffer,
Joining of nonhomologous DNA double strand breaks in vitro.
1988,
Pubmed
,
Xenbase
Roth,
Mechanisms of nonhomologous recombination in mammalian cells.
1985,
Pubmed
Roth,
Nonhomologous recombination in mammalian cells: role for short sequence homologies in the joining reaction.
1986,
Pubmed
Roth,
Oligonucleotide capture during end joining in mammalian cells.
1991,
Pubmed
Roth,
V(D)J recombination: broken DNA molecules with covalently sealed (hairpin) coding ends in scid mouse thymocytes.
1992,
Pubmed
Schiestl,
Transformation of Saccharomyces cerevisiae with nonhomologous DNA: illegitimate integration of transforming DNA into yeast chromosomes and in vivo ligation of transforming DNA to mitochondrial DNA sequences.
1993,
Pubmed
Szostak,
The double-strand-break repair model for recombination.
1983,
Pubmed
Thacker,
A mechanism for deletion formation in DNA by human cell extracts: the involvement of short sequence repeats.
1992,
Pubmed
Thode,
A novel pathway of DNA end-to-end joining.
1990,
Pubmed
,
Xenbase
Wake,
Topological requirements for homologous recombination among DNA molecules transfected into mammalian cells.
1985,
Pubmed
Winegar,
Spectrum of mutations produced by specific types of restriction enzyme-induced double-strand breaks.
1992,
Pubmed