Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Gen Genet
1994 Jun 15;2436:699-705. doi: 10.1007/bf00279580.
Show Gene links
Show Anatomy links
Drosophila melanogaster acetylcholinesterase: identification and expression of two mutations responsible for cold- and heat-sensitive phenotypes.
Mutero A
,
Bride JM
,
Pralavorio M
,
Fournier D
.
???displayArticle.abstract???
AceIJ29 and AceIJ40 are cold- and heat-sensitive variants of the gene coding for acetylcholinesterase in Drosophila melanogaster. In the homozygous condition, these mutations are lethal when animals are raised at restrictive temperatures, i.e., below 23 degrees C for AceIJ29 or above 25 degrees C for AceIJ40. The coding regions of the gene in these mutants were sequenced and mutations changing Ser374 to Phe in AceIJ29 and Pro75 to Leu in AceIJ40 were found. Acetylcholinesterases bearing these mutations were expressed in Xenopus oocytes and we found that these mutations decrease the secretion rate of the protein most probably by affecting its folding. This phenomenon is exacerbated at restrictive temperatures decreasing the amount of secreted acetylcholinesterase below the lethality threshold. In parallel, the substitution of the conserved Asp248 by an Asn residue completely inhibits the activity of the enzyme and its secretion, preventing the correct folding of the protein in a non-conditional manner.
ELLMAN,
A new and rapid colorimetric determination of acetylcholinesterase activity.
1961, Pubmed
ELLMAN,
A new and rapid colorimetric determination of acetylcholinesterase activity.
1961,
Pubmed
Fournier,
Drosophila acetylcholinesterase. Expression of a functional precursor in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Fournier,
Drosophila melanogaster acetylcholinesterase gene. Structure, evolution and mutations.
1989,
Pubmed
Fournier,
Insect glutathione S-transferases. Biochemical characteristics of the major forms from houseflies susceptible and resistant to insecticides.
1992,
Pubmed
Gausz,
Molecular Genetics of the ROSY-ACE Region of DROSOPHILA MELANOGASTER.
1986,
Pubmed
Greenspan,
Acetylcholinesterase mutants in Drosophila and their effects on the structure and function of the central nervous system.
1980,
Pubmed
Hall,
Genetics of acetylcholinesterase in Drosophila melanogaster.
1976,
Pubmed
Hall,
The Ace locus of Drosophila melanogaster: structural gene for acetylcholinesterase with an unusual 5' leader.
1986,
Pubmed
Hall,
Behavioral and biochemical defects in temperature-sensitive acetylcholinesterase mutants of Drosophila melanogaster.
1980,
Pubmed
Hoffmann,
Minigene rescues acetylcholinesterase lethal mutations in Drosophila melanogaster.
1992,
Pubmed
Holton,
A simple and efficient method for direct cloning of PCR products using ddT-tailed vectors.
1991,
Pubmed
Krejci,
Cholinesterase-like domains in enzymes and structural proteins: functional and evolutionary relationships and identification of a catalytically essential aspartic acid.
1991,
Pubmed
Nagoshi,
Molecular and recombinational mapping of mutations in the Ace locus of Drosophila melanogaster.
1987,
Pubmed
Nakamaye,
Inhibition of restriction endonuclease Nci I cleavage by phosphorothioate groups and its application to oligonucleotide-directed mutagenesis.
1986,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Sussman,
Atomic structure of acetylcholinesterase from Torpedo californica: a prototypic acetylcholine-binding protein.
1991,
Pubmed