XB-ART-20998
Cell Biol Int
1994 Aug 01;188:819-27. doi: 10.1006/cbir.1994.1115.
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Immunohistochemical characterization of a stage-specific antigen during oogenesis and spermatogenesis recognized with monoclonal antibody.
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In order to detect and characterize novel molecules which function in oogenesis, immunohistochemical study using a monoclonal antibody (MAb) raised against oocytes of Xenopus laevis was carried out. The distribution of the detectable molecule with MAb X-80, which specifically reacts with Xenopus previtellogenic oocytes; stages I and II (Dumont, 1972), in particular was analyzed. The hamster, mouse, chick, quail, Caenorhabditis elegans and Lilium longiflorum were also examined. Interestingly MAb X-80 bound not only in Xenopus oocytes but in its secondary spermatocytes and spermatids. Furthermore, MAb X-80 gave a similar staining pattern in the Lilium and other, although the stage when the positive reaction is detectable is different in the male and female germ cells.
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Species referenced: Xenopus laevis
Genes referenced: csf1