Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1994 Aug 16;9117:8278-82.
Show Gene links
Show Anatomy links
Characterization of glucose transport and cloning of a hexose transporter gene in Trypanosoma cruzi.
Tetaud E
,
Bringaud F
,
Chabas S
,
Barrett MP
,
Baltz T
.
???displayArticle.abstract???
A gene from Trypanosoma cruzi, TcrHT1, which encodes a member of the glucose transporter superfamily has been cloned. The gene is similar in sequence to the T. brucei hexose transporter THT1 and the Leishmania transporter Pro-1 and is present in the T. cruzi genome as a cluster of at least eight tandemly reiterated copies. Northern blot analysis revealed two mRNA transcripts which differ in size with respect to their 3' untranslated regions. When injected with in vitro transcribed TcrHT1 mRNA, Xenopus oocytes express a hexose transporter with properties similar to those of T. cruzi. Glucose transport in T. cruzi is mediated via a carrier with unique properties when compared with the other glucose transporters already characterized among the Kinetoplastida. It is a facilitated transporter with a high affinity for D-glucose (Km = 84.1 +/- 7.9 microM and Vmax = 46 +/- 9.4 nmol/min per mg of protein) that shares with other kinetoplastid hexose transporters the ability to recognize D-fructose, which distinguishes these carriers from the human erythrocyte glucose transporter GLUT1.
Baldwin,
Mammalian passive glucose transporters: members of an ubiquitous family of active and passive transport proteins.
1993, Pubmed
Baldwin,
Mammalian passive glucose transporters: members of an ubiquitous family of active and passive transport proteins.
1993,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Brener,
Biology of Trypanosoma cruzi.
1973,
Pubmed
Bringaud,
Differential regulation of two distinct families of glucose transporter genes in Trypanosoma brucei.
1993,
Pubmed
,
Xenbase
Bringaud,
African trypanosome glucose transporter genes: organization and evolution of a multigene family.
1994,
Pubmed
Bringaud,
A potential hexose transporter gene expressed predominantly in the bloodstream form of Trypanosoma brucei.
1992,
Pubmed
Cairns,
Developmentally regulated gene from Leishmania encodes a putative membrane transport protein.
1989,
Pubmed
Colville,
Kinetic analysis of the liver-type (GLUT2) and brain-type (GLUT3) glucose transporters in Xenopus oocytes: substrate specificities and effects of transport inhibitors.
1993,
Pubmed
,
Xenbase
Eisenthal,
Specificity and kinetics of hexose transport in Trypanosoma brucei.
1989,
Pubmed
Fry,
Transport of D-fructose and its analogues by Trypanosoma brucei.
1993,
Pubmed
Gould,
The glucose transporter family: structure, function and tissue-specific expression.
1993,
Pubmed
Gould,
Facilitative glucose transporters: an expanding family.
1990,
Pubmed
Higgins,
CLUSTAL: a package for performing multiple sequence alignment on a microcomputer.
1988,
Pubmed
Jennings,
Interaction between phloretin and the red blood cell membrane.
1976,
Pubmed
Jung,
Cytochalasin B binding sites and glucose transport carrier in human erythrocyte ghosts.
1977,
Pubmed
Knodler,
Glucose transport in Crithidia luciliae.
1992,
Pubmed
Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed
Langford,
Molecular characterization of two genes encoding members of the glucose transporter superfamily in the parasitic protozoan Leishmania donovani.
1992,
Pubmed
Opperdoes,
Localization of nine glycolytic enzymes in a microbody-like organelle in Trypanosoma brucei: the glycosome.
1977,
Pubmed
Parsons,
Active transport of 2-deoxy-D-glucose in Trypanosoma brucei procyclic forms.
1990,
Pubmed
Sanderson,
The growth of Trypanosoma cruzi in human diploid cells for the production of trypomastigotes.
1980,
Pubmed
Ter Kuile,
Uptake and turnover of glucose in Leishmania donovani.
1993,
Pubmed
Ter Kuile,
Glucose uptake by Trypanosoma brucei. Rate-limiting steps in glycolysis and regulation of the glycolytic flux.
1991,
Pubmed
Vera,
Functional expression of mammalian glucose transporters in Xenopus laevis oocytes: evidence for cell-dependent insulin sensitivity.
1989,
Pubmed
,
Xenbase
Vera,
Reconstitution of an insulin signaling pathway in Xenopus laevis oocytes: coexpression of a mammalian insulin receptor and three different mammalian hexose transporters.
1990,
Pubmed
,
Xenbase
Zilberstein,
Transport of nutrients and ions across membranes of trypanosomatid parasites.
1993,
Pubmed
Zilberstein,
Protonmotive force-driven active transport of D-glucose and L-proline in the protozoan parasite Leishmania donovani.
1985,
Pubmed
ter Kuile,
Mutual adjustment of glucose uptake and metabolism in Trypanosoma brucei grown in a chemostat.
1992,
Pubmed