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Mol Cell Biochem
1994 Aug 17;1371:75-83. doi: 10.1007/bf00926042.
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Studies on the iodination of a ras protein and the detection of ras polymers.
Chataway TK
,
Barritt GJ
.
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Several methods for the iodination of recombinant v-H-ras protein were compared. The Iodobead method gave greatest incorporation of radioactivity with minimal modification of the ras protein. Upon treatment of the ras protein with [125I] Nal and an Iodobead, radioactivity was initially incorporated into a 22 kDa species with a pl of 5.2, then predominantly into a 23 kDa species with a pl of 5.4. The specific activity of [125I]ras was 6 x 10(6) cpm/pmol total ras protein. Iondination did not alter the biological activity of the ras protein as judged by its ability to bind GTP gamma S and induce maturation of Xenopus laevis oocytes. It is concluded that while iodination alters the apparent molecular weight and pI of ras, presumably by the oxidation of one or more classes of amino acids, this does not affect the biological function of the protein. The ras protein, radioactively-labelled with iodine using the Iodobead method, should be suitable for studies of protein-protein interactions involving ras. Treatment of iodinated ras with the chemical cross-linking agent disuccinimidyl suberate revealed the presence of several minor high molecular weight protein species. This result shows that, in a dilute solution of purified ras protein, the monomeric form is in equilibrium with small amounts of polymeric forms.
Boguski,
Proteins regulating Ras and its relatives.
1993, Pubmed
Boguski,
Proteins regulating Ras and its relatives.
1993,
Pubmed
Downward,
Identification of a nucleotide exchange-promoting activity for p21ras.
1990,
Pubmed
Egan,
The pathway to signal achievement.
1993,
Pubmed
Feig,
The many roads that lead to Ras.
1993,
Pubmed
Fraker,
Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril.
1978,
Pubmed
Görg,
The current state of two-dimensional electrophoresis with immobilized pH gradients.
1988,
Pubmed
HUNTER,
Preparation of iodine-131 labelled human growth hormone of high specific activity.
1962,
Pubmed
Heward,
Effects of chloramine T and iodination on the biological activity of melanotropin.
1979,
Pubmed
Janknecht,
Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus.
1991,
Pubmed
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lowy,
Function and regulation of ras.
1993,
Pubmed
Marchalonis,
An enzymic method for the trace iodination of immunoglobulins and other proteins.
1969,
Pubmed
Markwell,
A new solid-state reagent to iodinate proteins. I. Conditions for the efficient labeling of antiserum.
1982,
Pubmed
McConahey,
Radioiodination of proteins by the use of the chloramine-T method.
1980,
Pubmed
Moodie,
Complexes of Ras.GTP with Raf-1 and mitogen-activated protein kinase kinase.
1993,
Pubmed
Morrison,
Catalysis of iodination by lactoperoxidase.
1970,
Pubmed
Morrison,
Peroxidase-catalyzed halogenation.
1976,
Pubmed
Nur-E-Kamal,
The GTPase-activating NF1 fragment of 91 amino acids reverses v-Ha-Ras-induced malignant phenotype.
1993,
Pubmed
Peterson,
A simplification of the protein assay method of Lowry et al. which is more generally applicable.
1977,
Pubmed
Santos,
Oligomeric structure of p21 ras proteins as determined by radiation inactivation.
1988,
Pubmed
Satoh,
Studies on ras proteins. Catalytic properties of normal and activated ras proteins purified in the absence of protein denaturants.
1988,
Pubmed
Shibuya,
Oncogenic ras triggers the activation of 42-kDa mitogen-activated protein kinase in extracts of quiescent Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Stagg,
Iodination and the biological activity of gastrin.
1970,
Pubmed
Switzer,
A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels.
1979,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Tucker,
Expression of p21 proteins in Escherichia coli and stereochemistry of the nucleotide-binding site.
1986,
Pubmed
