Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Genetics
1994 Oct 01;1382:445-57. doi: 10.1093/genetics/138.2.445.
Show Gene links
Show Anatomy links
Distribution of exchanges upon homologous recombination of exogenous DNA in Xenopus laevis oocytes.
Carroll D
,
Lehman CW
,
Jeong-Yu S
,
Dohrmann P
,
Dawson RJ
,
Trautman JK
.
???displayArticle.abstract???
Homologous recombination between DNA molecules injected into Xenopus oocyte nuclei was investigated by examining the recovery of information from differentially marked parental sequences. The injected recombination substrate was a linear DNA with terminal direct repeats of 1246 bp; one repeat differed from the other by eight single base-pair substitutions, distributed throughout the region of homology, each of which created or destroyed a restriction enzyme site. Recombination products were recovered and analyzed for their content of the diagnostic sites, either directly by Southern blot-hybridization or after cloning in bacteria. The majority (76%) of the cloned products appeared to be the result of simple exchanges-i.e., there was one sharp transition from sequences derived from one parent to sequences derived from the other. These simple exchanges were concentrated near the ends of the homologous interval and, thus, near the sites of the original molecular ends. Placing marked sites on only one side of the homologous overlap showed that marker recovery was governed largely by the positions of the molecular ends and not by the markers themselves. When a terminal nonhomology was present at one end of the substrate, the yield of recombinants was sharply decreased, but the pattern of exchanges was not affected, suggesting that products from end-blocked substrates arise by the same recombination pathway. Because of considerable evidence supporting a nonconservative, resection-annealing mechanism for recombination in oocytes, we interpret the distribution of exchanges as resulting from long-patch repair of extensive heteroduplex intermediates.
Abastado,
Intramolecular recombination between partially homologous sequences in Escherichia coli and Xenopus laevis oocytes.
1987, Pubmed,
Xenbase
Abastado,
Intramolecular recombination between partially homologous sequences in Escherichia coli and Xenopus laevis oocytes.
1987,
Pubmed
,
Xenbase
Anderson,
Recombination of homologous DNA fragments transfected into mammalian cells occurs predominantly by terminal pairing.
1986,
Pubmed
Bishop,
The role of heteroduplex correction in gene conversion in Saccharomyces cerevisiae.
,
Pubmed
Borts,
Meiotic recombination in yeast: alteration by multiple heterozygosities.
1987,
Pubmed
Brooks,
Mismatch repair involving localized DNA synthesis in extracts of Xenopus eggs.
1989,
Pubmed
,
Xenbase
Deng,
Mismatch repair of heteroduplex DNA intermediates of extrachromosomal recombination in mammalian cells.
1994,
Pubmed
Desautels,
Characterization of nonconservative homologous junctions in mammalian cells.
1990,
Pubmed
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed
Grzesiuk,
Recombination of DNAs in Xenopus oocytes based on short homologous overlaps.
1987,
Pubmed
,
Xenbase
Gurdon,
Gene transfer in amphibian eggs and oocytes.
1981,
Pubmed
Hays,
Rapid and apparently error-prone excision repair of nonreplicating UV-irradiated plasmids in Xenopus laevis oocytes.
1990,
Pubmed
,
Xenbase
Holmes,
Strand-specific mismatch correction in nuclear extracts of human and Drosophila melanogaster cell lines.
1990,
Pubmed
Jeong-Yu,
Effect of terminal nonhomologies on homologous recombination in Xenopus laevis oocytes.
1992,
Pubmed
,
Xenbase
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1987,
Pubmed
Lahue,
DNA mismatch correction in a defined system.
1989,
Pubmed
Längle-Rouault,
GATC sequences, DNA nicks and the MutH function in Escherichia coli mismatch repair.
1987,
Pubmed
Maryon,
Involvement of single-stranded tails in homologous recombination of DNA injected into Xenopus laevis oocyte nuclei.
1991,
Pubmed
,
Xenbase
Maryon,
Characterization of recombination intermediates from DNA injected into Xenopus laevis oocytes: evidence for a nonconservative mechanism of homologous recombination.
1991,
Pubmed
,
Xenbase
Pont-Kingdon,
Intermediates in extrachromosomal homologous recombination in Xenopus laevis oocytes: characterization by electron microscopy.
1993,
Pubmed
,
Xenbase
Russel,
An improved filamentous helper phage for generating single-stranded plasmid DNA.
1986,
Pubmed
Ryoji,
Chromatin assembly in Xenopus oocytes: in vivo studies.
1984,
Pubmed
,
Xenbase
Symington,
Expansions and contractions of the genetic map relative to the physical map of yeast chromosome III.
1988,
Pubmed
Varlet,
DNA mismatch repair in Xenopus egg extracts: repair efficiency and DNA repair synthesis for all single base-pair mismatches.
1990,
Pubmed
,
Xenbase
