XB-ART-20724
Eur J Endocrinol
1994 Oct 01;1314:385-90. doi: 10.1530/eje.0.1310385.
Show Gene links
Show Anatomy links
Molecular characterization of a cloned human oxytocin receptor.
???displayArticle.abstract???
We describe here the binding and functional properties of a cloned human oxytocin receptor (OTR). We established a transient OTR expression system on COS-1 cells, which do not express vasopressin receptors. With the transfected cells and [3H]oxytocin, the dissociation constant (Kd) of OTR to oxytocin was 6.0 +/- 1.1 nmol/l; the binding properties of several oxytocin-related peptides were also examined. The functional properties of OTR were determined by an electrophysiological method, using a Xenopus laevis oocyte injected with in vitro transcribed OTR mRNA. These two methods showed that [Phe2,Orn8]vasotocin, a vasopressin agonist, was an OTR antagonist. A combination of these methods using cloned OTR cDNA is a novel and effective method for the investigation of oxytocin-related ligands.
???displayArticle.pubmedLink??? 7921228
???displayArticle.link??? Eur J Endocrinol
Species referenced: Xenopus laevis
Genes referenced: avp oxt