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Cancer Chemother Pharmacol
1995 Jan 01;371-2:187-9. doi: 10.1007/bf00685648.
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Functional expression of the renal organic cation transporter and P-glycoprotein in Xenopus laevis oocytes.
Nelson JA
,
Dutt A
,
Allen LH
,
Wright DA
.
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The hypothesis that P-glycoprotein (P-gp) mediates the renal secretion of organic cations was tested by functional expression of mRNAs in the Xenopus laevis oocyte system. Efflux of 2'-deoxytubercidin (dTub), a substrate for the renal organic cation transporter (OCT) but not for P-gp, was enhanced by injection of renal mRNA but not by injection of mRNA from P-gp-overexpressing cells (MDCK cells transduced with the cDNA for human MDR1). The functional capacity of the MDCK-MDR mRNA was established by its ability to reduce the steady-state uptake of a classical P-gp substrate, vinblastine. Thus, these data indicate OCT and P-gp to be distinct entities. The Xenopus oocyte system provides a functional approach to further characterize the OCT.
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