Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Biosci
2005 Feb 01;301:11-4. doi: 10.1007/BF02705145.
Show Gene links
Show Anatomy links
The use of Xenopus oocytes and embryos as a route towards cell replacement.
Gurdon JB
.
???displayArticle.abstract???
When nuclei of somatic cells are transplanted to enucleated eggs of Xenopus, a complete reprogramming of nuclear function can take place. To identify mechanisms of nuclear reprogramming, somatic nuclei can be transplanted to growing meiotic oocytes of Xenopus, and stem cell genes activated without DNA replication. The combination of somatic cell nuclear transfer with morphogen signalling and the community effect may lead towards the possibility of cell replacement therapy. When mechanisms of nuclear reprogramming are understood, it may eventually be possible to directly reprogramme human somatic cell nuclei without the use of eggs.
Boiani,
Oct4 distribution and level in mouse clones: consequences for pluripotency.
2002, Pubmed
Boiani,
Oct4 distribution and level in mouse clones: consequences for pluripotency.
2002,
Pubmed
Bortvin,
Incomplete reactivation of Oct4-related genes in mouse embryos cloned from somatic nuclei.
2003,
Pubmed
Bourillot,
A changing morphogen gradient is interpreted by continuous transduction flow.
2002,
Pubmed
,
Xenbase
Briggs,
Transplantation of Living Nuclei From Blastula Cells into Enucleated Frogs' Eggs.
1952,
Pubmed
Byrne,
Nuclei of adult mammalian somatic cells are directly reprogrammed to oct-4 stem cell gene expression by amphibian oocytes.
2003,
Pubmed
,
Xenbase
De Robertis,
Gene activation in somatic nuclei after injection into amphibian oocytes.
1977,
Pubmed
,
Xenbase
Evans,
Establishment in culture of pluripotential cells from mouse embryos.
1981,
Pubmed
GURDON,
The developmental capacity of nuclei taken from intestinal epithelium cells of feeding tadpoles.
1962,
Pubmed
,
Xenbase
Gurdon,
The first half-century of nuclear transplantation.
2003,
Pubmed
Gurdon,
Morphogen gradient interpretation.
2001,
Pubmed
Gurdon,
Nuclear transplantation in eggs and oocytes.
1986,
Pubmed
Humpherys,
Abnormal gene expression in cloned mice derived from embryonic stem cell and cumulus cell nuclei.
2002,
Pubmed
Niwa,
Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells.
2000,
Pubmed
Rideout,
Nuclear cloning and epigenetic reprogramming of the genome.
2001,
Pubmed
Simonsson,
DNA demethylation is necessary for the epigenetic reprogramming of somatic cell nuclei.
2004,
Pubmed
,
Xenbase
Standley,
eFGF and its mode of action in the community effect during Xenopus myogenesis.
2001,
Pubmed
,
Xenbase