Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Receptor tyrosine phosphatases guide vertebrate motor axons during development.
Stepanek L
,
Stoker AW
,
Stoeckli E
,
Bixby JL
.
???displayArticle.abstract???
Receptor-type protein tyrosine phosphatases (RPTPs) are required for appropriate growth of axons during nervous system development in Drosophila. In the vertebrate, type IIa RPTPs [protein tyrosine phosphatase (PTP)-delta, PTP-sigma, and LAR (leukocyte common-antigen-related)] and the type III RPTP, PTP receptor type O (PTPRO), have been implicated in the regulation of axon growth, but their roles in developmental axon guidance are unclear. PTPRO, PTP-delta, and PTP-sigma are each expressed in chick motor neurons during the period of axonogenesis. To examine potential roles of RPTPs in axon growth and guidance in vivo, we used double-stranded RNA (dsRNA) interference combined with in ovo electroporation to knock down RPTP expression levels in the embryonic chick lumbar spinal cord. Although most branches of the developing limb nerves appeared grossly normal, a dorsal nerve identified as the anterior iliotibialis was clearly affected by dsRNA knock-down of RPTPs. In experimental embryos treated with dsRNA targeting PTP-delta, PTP-sigma, or PTPRO, this nerve showed abnormal fasciculation, was reduced in size, or was missing entirely; interference with PTPRO produced the most severe phenotypes. Control embryos electroporated with vehicle, or with dsRNA targeting choline acetyltransferase or axonin-1, did not exhibit this phenotype. Surprisingly, embryos electroporated with dsRNA targeting PTP-delta together with PTPRO, or all three RPTPs combined, had less severe phenotypes than embryos treated with PTPRO alone. This result suggests that competition between type IIa and type III RPTPs can regulate motor axon outgrowth, consistent with findings in Drosophila. Our results indicate that RPTPs, and especially PTPRO, are required for axon growth and guidance in the developing vertebrate limb.
Aricescu,
Heparan sulfate proteoglycans are ligands for receptor protein tyrosine phosphatase sigma.
2002, Pubmed
Aricescu,
Heparan sulfate proteoglycans are ligands for receptor protein tyrosine phosphatase sigma.
2002,
Pubmed
Beltran,
Expression of PTPRO during mouse development suggests involvement in axonogenesis and differentiation of NT-3 and NGF-dependent neurons.
2003,
Pubmed
Bixby,
Receptor tyrosine phosphatases in axon growth and guidance.
2000,
Pubmed
Bixby,
Ligands and signaling through receptor-type tyrosine phosphatases.
2001,
Pubmed
Bixby,
Extracellular matrix molecules and cell adhesion molecules induce neurites through different mechanisms.
1990,
Pubmed
Bodden,
CRYP-2: a receptor-type tyrosine phosphatase selectively expressed by developing vertebrate neurons.
1996,
Pubmed
Bron,
Functional knockdown of neuropilin-1 in the developing chick nervous system by siRNA hairpins phenocopies genetic ablation in the mouse.
2004,
Pubmed
Chang,
Inhibition of netrin-mediated axon attraction by a receptor protein tyrosine phosphatase.
2004,
Pubmed
Chen,
Neuronal pentraxin with chromo domain (NPCD) is a novel class of protein expressed in multiple neuronal domains.
2005,
Pubmed
Chen,
A novel substrate of receptor tyrosine phosphatase PTPRO is required for nerve growth factor-induced process outgrowth.
2005,
Pubmed
Chilton,
Expression of receptor protein tyrosine phosphatases in embryonic chick spinal cord.
2000,
Pubmed
Clandinin,
Drosophila LAR regulates R1-R6 and R7 target specificity in the visual system.
2001,
Pubmed
Desai,
Receptor tyrosine phosphatases are required for motor axon guidance in the Drosophila embryo.
1996,
Pubmed
Desai,
Competition and cooperation among receptor tyrosine phosphatases control motoneuron growth cone guidance in Drosophila.
1997,
Pubmed
Elbashir,
Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate.
2001,
Pubmed
Elchebly,
Neuroendocrine dysplasia in mice lacking protein tyrosine phosphatase sigma.
1999,
Pubmed
Garrity,
Retinal axon target selection in Drosophila is regulated by a receptor protein tyrosine phosphatase.
1999,
Pubmed
Gonzalez-Quevedo,
Receptor tyrosine phosphatase-dependent cytoskeletal remodeling by the hedgehog-responsive gene MIM/BEG4.
2005,
Pubmed
Hamburger,
Cell death in the development of the lateral motor column of the chick embryo.
1975,
Pubmed
Holen,
Positional effects of short interfering RNAs targeting the human coagulation trigger Tissue Factor.
2002,
Pubmed
Honig,
The development of sensory projection patterns in embryonic chick hindlimb under experimental conditions.
1986,
Pubmed
Honig,
In vitro studies of growth cone behavior support a role for fasciculation mediated by cell adhesion molecules in sensory axon guidance during development.
1998,
Pubmed
Johnson,
Receptor protein tyrosine phosphatases regulate retinal ganglion cell axon outgrowth in the developing Xenopus visual system.
2001,
Pubmed
,
Xenbase
Johnson,
Receptor protein tyrosine phosphatases in nervous system development.
2003,
Pubmed
Kaufmann,
Drosophila liprin-alpha and the receptor phosphatase Dlar control synapse morphogenesis.
2002,
Pubmed
Krueger,
The transmembrane tyrosine phosphatase DLAR controls motor axon guidance in Drosophila.
1996,
Pubmed
Lance-Jones,
Pathway selection by chick lumbosacral motoneurons during normal development.
1981,
Pubmed
Landmesser,
Altered sensory projections in the chick hind limb following the early removal of motoneurons.
1986,
Pubmed
Landmesser,
The distribution of motoneurones supplying chick hind limb muscles.
1978,
Pubmed
Landmesser,
The development of motor projection patterns in the chick hind limb.
1978,
Pubmed
Ledig,
Expression of receptor tyrosine phosphatases during development of the retinotectal projection of the chick.
1999,
Pubmed
Ledig,
The receptor tyrosine phosphatase CRYPalpha promotes intraretinal axon growth.
1999,
Pubmed
Lemmon,
The appearance of an L1-like molecule in the chick primary visual pathway.
1986,
Pubmed
Maurel-Zaffran,
Cell-autonomous and -nonautonomous functions of LAR in R7 photoreceptor axon targeting.
2001,
Pubmed
McLean,
Enhanced rate of nerve regeneration and directional errors after sciatic nerve injury in receptor protein tyrosine phosphatase sigma knock-out mice.
2002,
Pubmed
Milner,
Selective fasciculation and divergent pathfinding decisions of embryonic chick motor axons projecting to fast and slow muscle regions.
1998,
Pubmed
Misgeld,
Roles of neurotransmitter in synapse formation: development of neuromuscular junctions lacking choline acetyltransferase.
2002,
Pubmed
Newsome,
Analysis of Drosophila photoreceptor axon guidance in eye-specific mosaics.
2000,
Pubmed
Oppenheim,
The retrograde transport of horseradish peroxidase from the developing limb of the chick embryo.
1975,
Pubmed
Pekarik,
Screening for gene function in chicken embryo using RNAi and electroporation.
2003,
Pubmed
Rashid-Doubell,
Chick PTPsigma regulates the targeting of retinal axons within the optic tectum.
2002,
Pubmed
Schaeren-Wiemers,
A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probes.
1993,
Pubmed
Schindelholz,
Regulation of CNS and motor axon guidance in Drosophila by the receptor tyrosine phosphatase DPTP52F.
2001,
Pubmed
Schmid,
Combinatorial RNAi: a method for evaluating the functions of gene families in Drosophila.
2002,
Pubmed
Scott,
Skin sensory innervation patterns in embryonic chick hindlimb following dorsal root ganglion reversals.
1986,
Pubmed
Serra-Pagès,
The LAR transmembrane protein tyrosine phosphatase and a coiled-coil LAR-interacting protein co-localize at focal adhesions.
1995,
Pubmed
Sommer,
RPTP delta and the novel protein tyrosine phosphatase RPTP psi are expressed in restricted regions of the developing central nervous system.
1997,
Pubmed
Stepanek,
CRYP-2/cPTPRO is a neurite inhibitory repulsive guidance cue for retinal neurons in vitro.
2001,
Pubmed
Stoeckli,
Axonin-1, Nr-CAM, and Ng-CAM play different roles in the in vivo guidance of chick commissural neurons.
1995,
Pubmed
Stoker,
Axonal localisation of the CAM-like tyrosine phosphatase CRYP alpha: a signalling molecule of embryonic growth cones.
1995,
Pubmed
Sun,
Receptor tyrosine phosphatases regulate axon guidance across the midline of the Drosophila embryo.
2000,
Pubmed
Sun,
Growth cone steering by receptor tyrosine phosphatase delta defines a distinct class of guidance cue.
2000,
Pubmed
Sun,
Complex genetic interactions among four receptor tyrosine phosphatases regulate axon guidance in Drosophila.
2001,
Pubmed
Thompson,
Receptor protein tyrosine phosphatase sigma inhibits axonal regeneration and the rate of axon extension.
2003,
Pubmed
Tosney,
Specificity of early motoneuron growth cone outgrowth in the chick embryo.
1985,
Pubmed
Tsuchida,
Topographic organization of embryonic motor neurons defined by expression of LIM homeobox genes.
1994,
Pubmed
Uetani,
Impaired learning with enhanced hippocampal long-term potentiation in PTPdelta-deficient mice.
2000,
Pubmed
Van Vactor,
Genetic analysis of protein tyrosine phosphatases.
1998,
Pubmed
Van der Zee,
Delayed peripheral nerve regeneration and central nervous system collateral sprouting in leucocyte common antigen-related protein tyrosine phosphatase-deficient mice.
2003,
Pubmed
Wallace,
Neuronal defects and posterior pituitary hypoplasia in mice lacking the receptor tyrosine phosphatase PTPsigma.
1999,
Pubmed
Wang,
Receptor tyrosine phosphatase-delta is a homophilic, neurite-promoting cell adhesion molecular for CNS neurons.
1999,
Pubmed
Wang,
Muscle sensory innervation patterns in embryonic chick hindlimbs following dorsal root ganglion reversal.
1997,
Pubmed
Wyszynski,
Interaction between GRIP and liprin-alpha/SYD2 is required for AMPA receptor targeting.
2002,
Pubmed
Xie,
The leukocyte common antigen-related protein tyrosine phosphatase receptor regulates regenerative neurite outgrowth in vivo.
2001,
Pubmed
Yan,
A novel receptor tyrosine phosphatase-sigma that is highly expressed in the nervous system.
1993,
Pubmed
Yang,
Leukocyte antigen-related protein tyrosine phosphatase receptor: a small ectodomain isoform functions as a homophilic ligand and promotes neurite outgrowth.
2003,
Pubmed
Yeo,
Deficient LAR expression decreases basal forebrain cholinergic neuronal size and hippocampal cholinergic innervation.
1997,
Pubmed
Zhen,
The liprin protein SYD-2 regulates the differentiation of presynaptic termini in C. elegans.
1999,
Pubmed
