Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Cloning and functional expression of a rat kidney extracellular calcium/polyvalent cation-sensing receptor.
Riccardi D
,
Park J
,
Lee WS
,
Gamba G
,
Brown EM
,
Hebert SC
.
???displayArticle.abstract???
The maintenance of a stable extracellular concentration of ionized calcium depends on the integrated function of a number of specialized cells (e.g., parathyroid and certain kidney epithelial cells). We recently identified another G protein-coupled receptor (BoPCaRI) from bovine parathyroid that responds to changes in extracellular Ca2+ within the millimolar range and provides a key mechanism for regulating the secretion of parathyroid hormone. Using an homology-based strategy, we now report the isolation of a cDNA encoding an extracellular Ca2+/polyvalent cation-sensing receptor (RaKCaR) from rat kidney. The predicted RaKCaR protein shares 92% identity with BoPCaR1 receptor and features a seven membrane-spanning domain, characteristic of the G protein-coupled receptors, which is preceded by a large hydrophilic extracellular NH2 terminus believed to be involved in cation binding. RaKCaR cRNA-injected Xenopus oocytes responded to extracellular Ca2+, Mg2+, Gd3+, and neomycin with characteristic activation of inositol phospholipid-dependent, intracellular Ca(2+)-induced Cl- currents. In rat kidney, Northern analysis revealed RaKCaR transcripts of 4 and 7 kb, and in situ hybridization showed localization primarily in outer medulla and cortical medullary rays. Our results provide important insights into the molecular structure of an extracellular Ca2+/polyvalent cation-sensing receptor in rat kidney and provide another basis on which to understand the role of extracellular divalent cations in regulating kidney function in mineral metabolism.
Abe,
Molecular characterization of a novel metabotropic glutamate receptor mGluR5 coupled to inositol phosphate/Ca2+ signal transduction.
1992, Pubmed
Abe,
Molecular characterization of a novel metabotropic glutamate receptor mGluR5 coupled to inositol phosphate/Ca2+ signal transduction.
1992,
Pubmed
Attie,
Urinary calcium excretion in familial hypocalciuric hypercalcemia. Persistence of relative hypocalciuria after induction of hypoparathyroidism.
1983,
Pubmed
Black,
Regulation of goblet cell differentiation by calcium in embryonic chick intestine.
1989,
Pubmed
Brown,
Extracellular Ca2+ sensing, regulation of parathyroid cell function, and role of Ca2+ and other ions as extracellular (first) messengers.
1991,
Pubmed
Brown,
Cloning and characterization of an extracellular Ca(2+)-sensing receptor from bovine parathyroid.
1993,
Pubmed
,
Xenbase
Clemens,
Extracellular calcium modulates vitamin D-dependent calbindin-D28K gene expression in chick kidney cells.
1989,
Pubmed
Conklin,
Homeostatic signals. Marriage of the flytrap and the serpent.
1994,
Pubmed
Dascal,
The use of Xenopus oocytes for the study of ion channels.
1987,
Pubmed
,
Xenbase
Fliegel,
Amino acid sequence of rabbit fast-twitch skeletal muscle calsequestrin deduced from cDNA and peptide sequencing.
1987,
Pubmed
Fray,
Calcium and the control of renin secretion.
1987,
Pubmed
Gamba,
Primary structure and functional expression of a cDNA encoding the thiazide-sensitive, electroneutral sodium-chloride cotransporter.
1993,
Pubmed
,
Xenbase
Hediger,
Expression of size-selected mRNA encoding the intestinal Na/glucose cotransporter in Xenopus laevis oocytes.
1987,
Pubmed
,
Xenbase
Ho,
Cloning and expression of an inwardly rectifying ATP-regulated potassium channel.
1993,
Pubmed
,
Xenbase
Jones,
Effect of peritubular [Ca] or ionomycin on hydrosmotic response of CCTs to ADH or cAMP.
1988,
Pubmed
Kanai,
Expression of mRNA (D2) encoding a protein involved in amino acid transport in S3 proximal tubule.
1992,
Pubmed
,
Xenbase
Kozak,
Structural features in eukaryotic mRNAs that modulate the initiation of translation.
1991,
Pubmed
Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed
Marx,
Maximal urine-concentrating ability: familial hypocalciuric hypercalcemia versus typical primary hyperparathyroidism.
1981,
Pubmed
Masu,
Sequence and expression of a metabotropic glutamate receptor.
1991,
Pubmed
,
Xenbase
Mathias,
Divalent cations modulate PTH-dependent 3',5'-cyclic adenosine monophosphate production in renal proximal tubular cells.
1991,
Pubmed
Nakanishi,
Molecular diversity of glutamate receptors and implications for brain function.
1992,
Pubmed
Nemeth,
Cytosolic Ca2+ and the regulation of secretion in parathyroid cells.
1986,
Pubmed
O'Hara,
The ligand-binding domain in metabotropic glutamate receptors is related to bacterial periplasmic binding proteins.
1993,
Pubmed
Pollak,
Mutations in the human Ca(2+)-sensing receptor gene cause familial hypocalciuric hypercalcemia and neonatal severe hyperparathyroidism.
1993,
Pubmed
Quamme,
Control of magnesium transport in the thick ascending limb.
1989,
Pubmed
Slatopolsky,
Inhibitory effects of hypermagnesemia on the renal action of parathyroid hormone.
1976,
Pubmed
Takaichi,
High Ca2+ inhibits AVP-dependent cAMP production in thick ascending limbs of Henle.
1986,
Pubmed
Takaichi,
High Ca2+ inhibits peptide hormone-dependent cAMP production specifically in thick ascending limbs of Henle.
1986,
Pubmed
Takaichi,
Inhibitory guanosine triphosphate-binding protein-mediated regulation of vasopressin action in isolated single medullary tubules of mouse kidney.
1988,
Pubmed
Tanabe,
A family of metabotropic glutamate receptors.
1992,
Pubmed
Trechsel,
Calcium-dependent, parathyroid hormone-independent regulation of 1,25-dihydroxyvitamin D.
1980,
Pubmed
Weisinger,
Regulation of 1,25-dihydroxyvitamin D3 by calcium in the parathyroidectomized, parathyroid hormone-replete rat.
1989,
Pubmed
von Heijne,
A new method for predicting signal sequence cleavage sites.
1986,
Pubmed