Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1995 Feb 01;152:1094-101. doi: 10.1128/MCB.15.2.1094.
Show Gene links
Show Anatomy links
Activation of intracellular kinases in Xenopus oocytes by p21ras and phospholipases: a comparative study.
Carnero A
,
Lacal JC
.
???displayArticle.abstract???
Signal transduction induced by generations of second messengers from membrane phospholipids is a major regulatory mechanism in the control of cell proliferation. Indeed, oncogenic p21ras alters the intracellular levels of phospholipid metabolites in both mammalian cells and Xenopus oocytes. However, it is still controversial whether this alteration it is biologically significant. We have analyzed the ras-induced signal transduction pathway in Xenopus oocytes and have correlated its mechanism of activation with that of the three most relevant phospholipases (PLs). After microinjection, ras-p21 induces a rapid PLD activation followed by a late PLA2 activation. By contrast, phosphatidylcholine-specific PLC was not activated under similar conditions. When each of these PLs was studied for its ability to activate intracellular signalling kinases, all of them were found to activate maturation-promoting factor efficiently. However, only PLD was able to activate MAP kinase and S6 kinase II, a similar pattern to that induced by p21ras proteins. Thus, the comparison of activated enzymes after microinjection of p21ras or PLs indicated that only PLD microinjection mimetized p21ras signalling. Finally, inhibition of the endogenous PLD activity by neomycin substantially reduced the biological activity of p21ras. All these results suggest that PLD activation may constitute a relevant step in ras-induced germinal vesicle breakdown in Xenopus oocytes.
Allende,
Oncogenic ras protein induces meiotic maturation of amphibian oocytes in the presence of protein synthesis inhibitors.
1988, Pubmed,
Xenbase
Allende,
Oncogenic ras protein induces meiotic maturation of amphibian oocytes in the presence of protein synthesis inhibitors.
1988,
Pubmed
,
Xenbase
Barbacid,
ras genes.
1987,
Pubmed
Berridge,
Inositol trisphosphate and diacylglycerol: two interacting second messengers.
1987,
Pubmed
Billah,
The regulation and cellular functions of phosphatidylcholine hydrolysis.
1990,
Pubmed
Bourne,
The GTPase superfamily: conserved structure and molecular mechanism.
1991,
Pubmed
Carnero,
Phospholipase-induced maturation of Xenopus laevis oocytes: mitogenic activity of generated metabolites.
1993,
Pubmed
,
Xenbase
Carnero,
Activation of type D phospholipase by serum stimulation and ras-induced transformation in NIH3T3 cells.
1994,
Pubmed
Carnero,
ras-p21 activates phospholipase D and A2, but not phospholipase C or PKC, in Xenopus laevis oocytes.
1994,
Pubmed
,
Xenbase
Diaz-Laviada,
Evidence for a role of phosphatidylcholine-hydrolysing phospholipase C in the regulation of protein kinase C by ras and src oncogenes.
1990,
Pubmed
Erikson,
Structure, expression, and regulation of protein kinases involved in the phosphorylation of ribosomal protein S6.
1991,
Pubmed
,
Xenbase
Exton,
Signaling through phosphatidylcholine breakdown.
1990,
Pubmed
Ferrell,
Cell cycle tyrosine phosphorylation of p34cdc2 and a microtubule-associated protein kinase homolog in Xenopus oocytes and eggs.
1991,
Pubmed
,
Xenbase
García de Herreros,
Requirement of phospholipase C-catalyzed hydrolysis of phosphatidylcholine for maturation of Xenopus laevis oocytes in response to insulin and ras p21.
1991,
Pubmed
,
Xenbase
Gotoh,
Xenopus M phase MAP kinase: isolation of its cDNA and activation by MPF.
1991,
Pubmed
,
Xenbase
Hoffman,
Orthotopic is orthodox: why are orthotopic-transplant metastatic models different from all other models?
1994,
Pubmed
Hoshi,
Characterization of a mitogen-activated, Ca2+-sensitive microtubule-associated protein-2 kinase.
1989,
Pubmed
Hoshi,
Activation of a Ca2+-inhibitable protein kinase that phosphorylates microtubule-associated protein 2 in vitro by growth factors, phorbol esters, and serum in quiescent cultured human fibroblasts.
1988,
Pubmed
Jacobs,
Control of the cell cycle.
1992,
Pubmed
Jiang,
Evidence that v-Src-induced phospholipase D activity is mediated by a G protein.
1994,
Pubmed
Kosako,
Requirement for the MAP kinase kinase/MAP kinase cascade in Xenopus oocyte maturation.
1994,
Pubmed
,
Xenbase
Lacal,
Diacylglycerol production in Xenopus laevis oocytes after microinjection of p21ras proteins is a consequence of activation of phosphatidylcholine metabolism.
1990,
Pubmed
,
Xenbase
Lacal,
Ras p21 proteins with high or low GTPase activity can efficiently transform NIH/3T3 cells.
1986,
Pubmed
Lacal,
Novel source of 1,2-diacylglycerol elevated in cells transformed by Ha-ras oncogene.
,
Pubmed
Leevers,
Activation of extracellular signal-regulated kinase, ERK2, by p21ras oncoprotein.
1992,
Pubmed
Lin,
cPLA2 is phosphorylated and activated by MAP kinase.
1993,
Pubmed
Liscovitch,
Inhibition of neural phospholipase D activity by aminoglycoside antibiotics.
1991,
Pubmed
Lopez-Barahona,
Kinetic evidence of a rapid activation of phosphatidylcholine hydrolysis by Ki-ras oncogene. Possible involvement in late steps of the mitogenic cascade.
1990,
Pubmed
Maller,
Xenopus oocytes and the biochemistry of cell division.
1990,
Pubmed
,
Xenbase
Marshall,
MAP kinase kinase kinase, MAP kinase kinase and MAP kinase.
1994,
Pubmed
Mulcahy,
Requirement for ras proto-oncogene function during serum-stimulated growth of NIH 3T3 cells.
,
Pubmed
Nebreda,
p21ras-induced meiotic maturation of Xenopus oocytes in the absence of protein synthesis: MPF activation is preceded by activation of MAP and S6 kinases.
1993,
Pubmed
,
Xenbase
Nishida,
The MAP kinase cascade is essential for diverse signal transduction pathways.
1993,
Pubmed
,
Xenbase
Nishizuka,
Intracellular signaling by hydrolysis of phospholipids and activation of protein kinase C.
1992,
Pubmed
Norbury,
Animal cell cycles and their control.
1992,
Pubmed
Pan,
Role of phosphatidylinositide metabolism in ras-induced Xenopus oocyte maturation.
1990,
Pubmed
,
Xenbase
Pelech,
Mitogen-activated protein kinases: versatile transducers for cell signaling.
1992,
Pubmed
Plevin,
Multiple sources of sn-1,2-diacylglycerol in platelet-derived-growth-factor-stimulated Swiss 3T3 fibroblasts. Evidence for activation of phosphoinositidase C and phosphatidylcholine-specific phospholipase D.
1991,
Pubmed
Qui,
PC12 cell neuronal differentiation is associated with prolonged p21ras activity and consequent prolonged ERK activity.
1992,
Pubmed
Ray,
Rapid stimulation by insulin of a serine/threonine kinase in 3T3-L1 adipocytes that phosphorylates microtubule-associated protein 2 in vitro.
1987,
Pubmed
Ray,
Characterization of insulin-stimulated microtubule-associated protein kinase. Rapid isolation and stabilization of a novel serine/threonine kinase from 3T3-L1 cells.
1988,
Pubmed
Rossomando,
Evidence that pp42, a major tyrosine kinase target protein, is a mitogen-activated serine/threonine protein kinase.
1989,
Pubmed
Shibuya,
Oncogenic ras triggers the activation of 42-kDa mitogen-activated protein kinase in extracts of quiescent Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Song,
v-Src increases diacylglycerol levels via a type D phospholipase-mediated hydrolysis of phosphatidylcholine.
1991,
Pubmed
Sturgill,
Insulin-stimulated MAP-2 kinase phosphorylates and activates ribosomal protein S6 kinase II.
1988,
Pubmed
,
Xenbase
Tsao,
The roles of macromolecular synthesis and phosphorylation in the regulation of a protein kinase activity transiently stimulated by nerve growth factor.
1991,
Pubmed
Volonté,
Differential inhibition of nerve growth factor responses by purine analogues: correlation with inhibition of a nerve growth factor-activated protein kinase.
1989,
Pubmed
