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XB-ART-19797
Biophys J 1995 May 01;685:1687-93.
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A look at membrane patches with a scanning force microscope.

Hörber JK , Mosbacher J , Häberle W , Ruppersberg JP , Sakmann B .


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We combined scanning force microscopy with patch-clamp techniques in the same experimental setup and obtained images of excised membrane patches spanning the tip of a glass pipette. These images indicate that cytoskeleton structures are still present in such membrane patches and form a strong connection between the membrane and the glass wall. This gives the membrane patch the appearance of a tent, stabilized by a scaffold of ropes. The lateral resolution of the images depends strongly on the observed structures and can reach values as low as 10 nm on the cytoskeleton elements of a (inside-out) patch. The observations suggest that measurements of membrane elasticity can be made, opening the way for further studies on mechanical properties of cell membranes.

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References [+] :
Bezanilla, Motion and enzymatic degradation of DNA in the atomic force microscope. 1994, Pubmed