Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Additive effect of ADP and CGRP in modulation of the acetylcholine receptor channel in Xenopus embryonic myocytes.
Liou JC, Fu WM.
???displayArticle.abstract???
1. We have previously shown that the activation of either protein kinase A (PKA) or protein kinase C (PKC) enhanced the responses of muscle membrane to acetylcholine (ACh) by increasing the mean open time of embryonic-type ACh channels in Xenopus cultured myocytes. In the present study, we further investigated the interaction between these two kinases in the modulation of ACh channels by using the receptor ligands, adenosine diphosphate (ADP) and calcitonin gene-related peptide (CGRP) which selectively activate PKC and PKA, respectively. 2. ADP concentration-dependently increased the mean open time of embryonic-type ACh channels and 0.3 mM ADP is sufficient to achieve the maximal potentiating effect. alpha, beta-Methylene ATP and PMA (phorbol 12-myristate 13-acetate) but not adenosine, AMP, dibutyryl cyclic GMP have similar potentiating action. 3. Suramin (0.3 mM) pretreatment abolished the potentiating effect of ADP but left that of PMA unchanged. 4. CGRP increased the mean open time of embryonic-type ACh channels in a concentration-dependent manner and 1 microM CGRP produced the maximal effect. 5. The maximal effects of both ADP (0.3 mM) and CGRP (1 microM) in the prolongation of mean open time of ACh channels were additive. 6. These results suggest that the modulation of embryonic-type ACh channels by the endogenously released ligands via the activation of PKA and PKC is additive and possibly different sites of ACh channels may be involved in the potentiation effect of either PKC or PKA.
Albuquerque,
A possible involvement of cyclic AMP in the expression of desensitization of the nicotinic acetylcholine receptor. A study with forskolin and its analogs.
1986, Pubmed
Albuquerque,
A possible involvement of cyclic AMP in the expression of desensitization of the nicotinic acetylcholine receptor. A study with forskolin and its analogs.
1986,
Pubmed Brehm,
Acetylcholine receptor channel properties during development of Xenopus muscle cells in culture.
1984,
Pubmed
,
Xenbase Bridgman,
Freeze-fracture and electrophysiological studies of newly developed acetylcholine receptors in Xenopus embryonic muscle cells.
1984,
Pubmed
,
Xenbase Changeux,
Acetylcholine receptor: an allosteric protein.
1984,
Pubmed Dowdall,
Adenosine triphosphate. A constituent of cholinergic synaptic vesicles.
1974,
Pubmed Dubyak,
Signal transduction via P2-purinergic receptors for extracellular ATP and other nucleotides.
1993,
Pubmed Eusebi,
Agents that activate protein kinase C reduce acetylcholine sensitivity in cultured myotubes.
1985,
Pubmed Fu,
Potentiation of acetylcholine responses in Xenopus embryonic muscle cells by dibutyryl cAMP.
1993,
Pubmed
,
Xenbase Fu,
ATP potentiates spontaneous transmitter release at developing neuromuscular synapses.
1991,
Pubmed
,
Xenbase Fu,
Potentiation by ATP of the postsynaptic acetylcholine response at developing neuromuscular synapses in Xenopus cell cultures.
1994,
Pubmed
,
Xenbase Fu,
Developmental change in the modulation of acetylcholine receptor channel by protein kinase C activation in Xenopus embryonic muscle cells.
1993,
Pubmed
,
Xenbase Fu,
Activation of protein kinase C potentiates postsynaptic acetylcholine response at developing neuromuscular synapses.
1993,
Pubmed
,
Xenbase Galzi,
Functional architecture of the nicotinic acetylcholine receptor: from electric organ to brain.
1991,
Pubmed Gordon,
Phosphorylation of acetylcholine receptor by endogenous membrane protein kinase in receptor-enriched membranes of Torpedo californica.
1977,
Pubmed Greengard,
Phosphorylated proteins as physiological effectors.
1978,
Pubmed Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed Hemmings,
Role of protein phosphorylation in neuronal signal transduction.
1989,
Pubmed Hopfield,
Functional modulation of the nicotinic acetylcholine receptor by tyrosine phosphorylation.
1988,
Pubmed Hoyle,
Suramin antagonizes responses to P2-purinoceptor agonists and purinergic nerve stimulation in the guinea-pig urinary bladder and taenia coli.
1990,
Pubmed Huganir,
Protein phosphorylation of nicotinic acetylcholine receptors.
1989,
Pubmed Huganir,
Phosphorylation of the nicotinic acetylcholine receptor by an endogenous tyrosine-specific protein kinase.
1984,
Pubmed Huganir,
cAMP-dependent protein kinase phosphorylates the nicotinic acetylcholine receptor.
1983,
Pubmed Hume,
Excitatory action of ATP on embryonic chick muscle.
1986,
Pubmed Kennedy,
Experimental approaches to understanding the role of protein phosphorylation in the regulation of neuronal function.
1983,
Pubmed Kidokoro,
Early cross-striation formation in twitching Xenopus myocytes in culture.
1988,
Pubmed
,
Xenbase Laufer,
Calcitonin gene-related peptide elevates cyclic AMP levels in chick skeletal muscle: possible neurotrophic role for a coexisting neuronal messenger.
1987,
Pubmed Leonard,
Differential development of two classes of acetylcholine receptors in Xenopus muscle in culture.
1984,
Pubmed
,
Xenbase Lo,
Activity-dependent synaptic competition in vitro: heterosynaptic suppression of developing synapses.
1991,
Pubmed
,
Xenbase Lu,
Calcitonin gene-related peptide potentiates synaptic responses at developing neuromuscular junction.
1993,
Pubmed
,
Xenbase Middleton,
Desensitization of acetylcholine receptors in rat myotubes is enhanced by agents that elevate intracellular cAMP.
1988,
Pubmed Middleton,
Forskolin increases the rate of acetylcholine receptor desensitization at rat soleus endplates.
1986,
Pubmed Miles,
Calcitonin gene-related peptide regulates phosphorylation of the nicotinic acetylcholine receptor in rat myotubes.
1989,
Pubmed Mulle,
Calcitonin gene-related peptide enhances the rate of desensitization of the nicotinic acetylcholine receptor in cultured mouse muscle cells.
1988,
Pubmed Nishizuka,
Turnover of inositol phospholipids and signal transduction.
1984,
Pubmed Owens,
In vivo development of nicotinic acetylcholine receptor channels in Xenopus myotomal muscle.
1989,
Pubmed
,
Xenbase Peng,
Development of calcitonin gene-related peptide (CGRP) immunoreactivity in relationship to the formation of neuromuscular junctions in Xenopus myotomal muscle.
1989,
Pubmed
,
Xenbase Qu,
Regulation of tyrosine phosphorylation of the nicotinic acetylcholine receptor at the rat neuromuscular junction.
1990,
Pubmed Rohrbough,
Changes in kinetics of acetylcholine receptor channels after initial expression in Xenopus myocyte culture.
1990,
Pubmed
,
Xenbase Ross,
Induction of phosphorylation and cell surface redistribution of acetylcholine receptors by phorbol ester and carbamylcholine in cultured chick muscle cells.
1988,
Pubmed Safran,
Phosphorylation of the acetylcholine receptor by protein kinase C and identification of the phosphorylation site within the receptor delta subunit.
1987,
Pubmed Sanes,
In vitro analysis of position- and lineage-dependent selectivity in the formation of neuromuscular synapses.
1989,
Pubmed
,
Xenbase Smith,
Regulation of phosphorylation of nicotinic acetylcholine receptors in mouse BC3H1 myocytes.
1987,
Pubmed Teichberg,
In vitro phosphorylation of the acetylcholine receptor.
1977,
Pubmed Uchida,
Release of calcitonin gene-related peptide-like immunoreactive substance from neuromuscular junction by nerve excitation and its action on striated muscle.
1990,
Pubmed Wallace,
Agrin induces phosphorylation of the nicotinic acetylcholine receptor.
1991,
Pubmed Xie,
Initial events in the formation of neuromuscular synapse: rapid induction of acetylcholine release from embryonic neuron.
1986,
Pubmed
,
Xenbase Yee,
Determination of the sites of cAMP-dependent phosphorylation on the nicotinic acetylcholine receptor.
1987,
Pubmed Zimmermann,
Turnover of adenine nucleotides in cholinergic synaptic vesicles of the Torpedo electric organ.
1978,
Pubmed
