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Dissection of transcription factor TFIIF functional domains required for initiation and elongation.
Tan S
,
Conaway RC
,
Conaway JW
.
???displayArticle.abstract??? TFIIF is unique among the general transcription factors because of its ability to control the activity of RNA polymerase II at both the initiation and elongation stages of transcription. Mammalian TFIIF, a heterodimer of approximately 30-kDa (RAP30) and approximately 70-kDa (RAP74) subunits, assists TFIIB in recruiting RNA polymerase II into the preinitiation complex and activates the overall rate of RNA chain elongation by suppressing transient pausing by polymerase at many sites on DNA templates. A major objective of efforts to understand how TFIIF regulates transcription has been to establish the relationship between its initiation and elongation activities. Here we establish this relationship by demonstrating that TFIIF transcriptional activities are mediated by separable functional domains. To accomplish this, we sought and identified distinct classes of RAP30 mutations that selectively block TFIIF activity in transcription initiation and elongation. We propose that (i) TFIIF initiation activity is mediated at least in part by RAP30 C-terminal sequences that include a cryptic DNA-binding domain similar to conserved region 4 of bacterial sigma factors and (ii) TFIIF elongation activity is mediated in part by RAP30 sequences located immediately upstream of the C terminus in a region proposed to bind RNA polymerase II and by additional sequences located in the RAP30 N terminus.
Bradsher,
RNA polymerase II transcription factor SIII. I. Identification, purification, and properties.
1993, Pubmed
Bradsher,
RNA polymerase II transcription factor SIII. I. Identification, purification, and properties.
1993,
Pubmed
Bradsher,
RNA polymerase II transcription factor SIII. II. Functional properties and role in RNA chain elongation.
1993,
Pubmed
Buratowski,
RNA polymerase II-associated proteins are required for a DNA conformation change in the transcription initiation complex.
1991,
Pubmed
Conaway,
Mechanism of assembly of the RNA polymerase II preinitiation complex. Transcription factors delta and epsilon promote stable binding of the transcription apparatus to the initiator element.
1992,
Pubmed
Conaway,
ATP activates transcription initiation from promoters by RNA polymerase II in a reversible step prior to RNA synthesis.
1988,
Pubmed
Conaway,
A multisubunit transcription factor essential for accurate initiation by RNA polymerase II.
1989,
Pubmed
Conaway,
Transcription initiated by RNA polymerase II and purified transcription factors from liver. Transcription factors alpha, beta gamma, and delta promote formation of intermediates in assembly of the functional preinitiation complex.
1990,
Pubmed
Conaway,
Mechanism of promoter selection by RNA polymerase II: mammalian transcription factors alpha and beta gamma promote entry of polymerase into the preinitiation complex.
1991,
Pubmed
Conaway,
General initiation factors for RNA polymerase II.
1993,
Pubmed
Coulombe,
Topological localization of the human transcription factors IIA, IIB, TATA box-binding protein, and RNA polymerase II-associated protein 30 on a class II promoter.
1994,
Pubmed
Flores,
Factors involved in specific transcription by mammalian RNA polymerase II. Factors IIE and IIF independently interact with RNA polymerase II.
1989,
Pubmed
Flores,
The small subunit of transcription factor IIF recruits RNA polymerase II into the preinitiation complex.
1991,
Pubmed
Flores,
Factors involved in specific transcription by mammalian RNA polymerase II. Identification and characterization of factor IIH.
1992,
Pubmed
Garrett,
The carboxyl terminus of RAP30 is similar in sequence to region 4 of bacterial sigma factors and is required for function.
1992,
Pubmed
Gong,
Imperfect conservation of a sigma factor-like subregion in Xenopus general transcription factor RAP30.
1992,
Pubmed
,
Xenbase
Goodrich,
Transcription factors IIE and IIH and ATP hydrolysis direct promoter clearance by RNA polymerase II.
1994,
Pubmed
Ha,
Multiple functional domains of human transcription factor IIB: distinct interactions with two general transcription factors and RNA polymerase II.
1993,
Pubmed
Henry,
TFIIF-TAF-RNA polymerase II connection.
1994,
Pubmed
Horikoshi,
Nucleotide and amino acid sequence of RAP30.
1991,
Pubmed
Izban,
Factor-stimulated RNA polymerase II transcribes at physiological elongation rates on naked DNA but very poorly on chromatin templates.
1992,
Pubmed
Kadesch,
Studies of in vitro transcription by calf thymus RNA polymerase II using a novel duplex DNA template.
1982,
Pubmed
Kash,
Control of transcription arrest in intron 1 of the murine adenosine deaminase gene.
1994,
Pubmed
Kato,
HIV-1 Tat acts as a processivity factor in vitro in conjunction with cellular elongation factors.
1992,
Pubmed
Kephart,
Functional analysis of Drosophila factor 5 (TFIIF), a general transcription factor.
1994,
Pubmed
Kerppola,
RNA polymerase: regulation of transcript elongation and termination.
1991,
Pubmed
Kim,
A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II.
1994,
Pubmed
Kobayashi,
Isolation and nucleotide sequence of a rat cDNA homologous to human RAP30.
1992,
Pubmed
Koleske,
An RNA polymerase II holoenzyme responsive to activators.
1994,
Pubmed
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1985,
Pubmed
Lesley,
Characterization of the Escherichia coli transcription factor sigma 70: localization of a region involved in the interaction with core RNA polymerase.
1989,
Pubmed
Maxon,
Transcription factor IIE binds preferentially to RNA polymerase IIa and recruits TFIIH: a model for promoter clearance.
1994,
Pubmed
McCracken,
Related RNA polymerase-binding regions in human RAP30/74 and Escherichia coli sigma 70.
1991,
Pubmed
Mote,
A DNA minor groove-binding ligand both potentiates and arrests transcription by RNA polymerase II. Elongation factor SII enables readthrough at arrest sites.
1994,
Pubmed
Parvin,
Multiple sets of basal factors initiate transcription by RNA polymerase II.
1994,
Pubmed
Parvin,
DNA topology and a minimal set of basal factors for transcription by RNA polymerase II.
1993,
Pubmed
Price,
Dynamic interaction between a Drosophila transcription factor and RNA polymerase II.
1989,
Pubmed
Reines,
Elongation factor SII-dependent transcription by RNA polymerase II through a sequence-specific DNA-binding protein.
1993,
Pubmed
Reines,
Transcription elongation factor SII (TFIIS) enables RNA polymerase II to elongate through a block to transcription in a human gene in vitro.
1989,
Pubmed
Rice,
Footprinting analysis of mammalian RNA polymerase II along its transcript: an alternative view of transcription elongation.
1991,
Pubmed
Schuler,
A workbench for multiple alignment construction and analysis.
1991,
Pubmed
Sekimizu,
Purification of a factor from Ehrlich ascites tumor cells specifically stimulating RNA polymerase II.
1976,
Pubmed
SivaRaman,
Purified elongation factor SII is sufficient to promote read-through by purified RNA polymerase II at specific termination sites in the human histone H3.3 gene.
1990,
Pubmed
Sopta,
Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II.
1989,
Pubmed
Tan,
A bacteriophage vector suitable for site-directed mutagenesis and high-level expression of multisubunit proteins in E. coli.
1994,
Pubmed
Tan,
Roles for both the RAP30 and RAP74 subunits of transcription factor IIF in transcription initiation and elongation by RNA polymerase II.
1994,
Pubmed
Tan,
Cryptic DNA-binding domain in the C terminus of RNA polymerase II general transcription factor RAP30.
1994,
Pubmed
Timmers,
Transcription initiation by RNA polymerase II does not require hydrolysis of the beta-gamma phosphoanhydride bond of ATP.
1994,
Pubmed
Tyree,
Identification of a minimal set of proteins that is sufficient for accurate initiation of transcription by RNA polymerase II.
1993,
Pubmed
Wang,
Production of human RAP30 and RAP74 in bacterial cells.
1993,
Pubmed
Wiest,
Mechanistic studies of transcription arrest at the adenovirus major late attenuation site. Comparison of purified RNA polymerase II and washed elongation complexes.
1992,
Pubmed
Yonaha,
Domain structure of a human general transcription initiation factor, TFIIF.
1993,
Pubmed