Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
RNA
2005 Jun 01;116:958-65. doi: 10.1261/rna.2020805.
Show Gene links
Show Anatomy links
The poly(A)-limiting element enhances mRNA accumulation by increasing the efficiency of pre-mRNA 3' processing.
Peng J
,
Murray EL
,
Schoenberg DR
.
???displayArticle.abstract???
The poly(A)-limiting element (PLE) is a conserved sequence originally found in the 3' UTR of Xenopus albumin mRNA whose presence restricts the length of the poly(A) tail on both pre-mRNA and fully processed mRNA to <20 nt. Results presented in this study show that the PLE also increases the cytoplasmic level of reporter beta-globin mRNA. Transcription run-on shows this increase was not due to increased reporter gene transcription, and experiments with tetracycline repressor-controlled reporter mRNA showed the PLE does not alter the rate of mRNA decay. Both RT-PCR and RNase protection assay showed the PLE caused a 50% increase in the 3' processing of reporter beta-globin mRNA in vivo. This was confirmed in vitro, where PLE-containing RNA was cleaved in HeLa nuclear extract at a rate 80% faster than a control RNA bearing an inactive element. These results indicate that the PLE regulates the length of the poly(A) tail and the efficiency of 3' processing. In addition, they show that PLE-containing mRNA with a <20-nt poly(A) tail is as stable as mRNA with a 100- to 200-nt poly(A) tail.
Choi,
Purifying mRNAs with a high-affinity eIF4E mutant identifies the short 3' poly(A) end phenotype.
2003, Pubmed
Choi,
Purifying mRNAs with a high-affinity eIF4E mutant identifies the short 3' poly(A) end phenotype.
2003,
Pubmed
Das Gupta,
Identification of two cis-acting elements that independently regulate the length of poly(A) on Xenopus albumin pre-mRNA.
1998,
Pubmed
,
Xenbase
Edmonds,
A history of poly A sequences: from formation to factors to function.
2002,
Pubmed
Gehring,
Increased efficiency of mRNA 3' end formation: a new genetic mechanism contributing to hereditary thrombophilia.
2001,
Pubmed
Gu,
U2AF modulates poly(A) length control by the poly(A)-limiting element.
2003,
Pubmed
Gu,
The poly(A)-limiting element is a conserved cis-acting sequence that regulates poly(A) tail length on nuclear pre-mRNAs.
1999,
Pubmed
,
Xenbase
Gupta,
Position and sequence requirements for poly(A) length regulation by the poly(A) limiting element.
2001,
Pubmed
Juge,
Control of poly(A) polymerase level is essential to cytoplasmic polyadenylation and early development in Drosophila.
2002,
Pubmed
Levitt,
Definition of an efficient synthetic poly(A) site.
1989,
Pubmed
Lewis,
Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets.
2005,
Pubmed
Peng,
mRNA with a <20-nt poly(A) tail imparted by the poly(A)-limiting element is translated as efficiently in vivo as long poly(A) mRNA.
2005,
Pubmed
Rao,
Regulated nuclear polyadenylation of Xenopus albumin pre-mRNA.
1996,
Pubmed
,
Xenbase
Sallés,
Assaying the polyadenylation state of mRNAs.
1999,
Pubmed
Schoenberg,
Extranuclear estrogen-regulated destabilization of Xenopus laevis serum albumin mRNA.
1989,
Pubmed
,
Xenbase
Sheth,
Decapping and decay of messenger RNA occur in cytoplasmic processing bodies.
2003,
Pubmed
Tharun,
Targeting an mRNA for decapping: displacement of translation factors and association of the Lsm1p-7p complex on deadenylated yeast mRNAs.
2001,
Pubmed
Wilusz,
The cap-to-tail guide to mRNA turnover.
2001,
Pubmed
Yang,
Endonuclease-mediated mRNA decay requires tyrosine phosphorylation of polysomal ribonuclease 1 (PMR1) for the targeting and degradation of polyribosome-bound substrate mRNA.
2004,
Pubmed
,
Xenbase
Zhao,
Formation of mRNA 3' ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis.
1999,
Pubmed
