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Membrane/microtubule tip attachment complexes (TACs) allow the assembly dynamics of plus ends to push and pull membranes into tubulovesicular networks in interphase Xenopus egg extracts.
Waterman-Storer CM
,
Gregory J
,
Parsons SF
,
Salmon ED
.
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We discovered by using high resolution video microscopy, that membranes become attached selectively to the growing plus ends of microtubules by membrane/microtubule tip attachment complexes (TACs) in interphase-arrested, undiluted, Xenopus egg extracts. Persistent plus end growth of stationary microtubules pushed the membranes into thin tubules and dragged them through the cytoplasm at the approximately 20 microns/min velocity typical of free plus ends. Membrane tubules also remained attached to plus ends when they switched to the shortening phase of dynamic instability at velocities typical of free ends, 50-60 microns/min. Over time, the membrane tubules contacted and fused with one another along their lengths, forming a polygonal network much like the distribution of ER in cells. Several components of the membrane networks formed by TACs were identified as ER by immunofluorescent staining using antibodies to ER-resident proteins. TAC motility was not inhibited by known inhibitors of microtubule motor activity, including 5 mM AMP-PNP, 250 microM orthovanadate, and ATP depletion. These results show that membrane/microtubule TACs enable polymerizing ends to push and depolymerizing ends to pull membranes into thin tubular extensions and networks at fast velocities.
Allan,
Cell cycle control of microtubule-based membrane transport and tubule formation in vitro.
1991, Pubmed,
Xenbase
Allan,
Cell cycle control of microtubule-based membrane transport and tubule formation in vitro.
1991,
Pubmed
,
Xenbase
Allan,
Movement of membrane tubules along microtubules in vitro: evidence for specialised sites of motor attachment.
1994,
Pubmed
,
Xenbase
Bell,
Preparation and purification of dynein.
1982,
Pubmed
Belmont,
Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts.
1990,
Pubmed
,
Xenbase
Caplow,
Microtubule dynamics.
1992,
Pubmed
Cassimeris,
Real-time observations of microtubule dynamic instability in living cells.
1988,
Pubmed
Chandra,
An N-terminal truncation of the ncd motor protein supports diffusional movement of microtubules in motility assays.
1993,
Pubmed
Cooper,
Tubulovesicular processes emerge from trans-Golgi cisternae, extend along microtubules, and interlink adjacent trans-golgi elements into a reticulum.
1990,
Pubmed
Cortese,
Actin polymerization induces a shape change in actin-containing vesicles.
1989,
Pubmed
Coue,
Microtubule depolymerization promotes particle and chromosome movement in vitro.
1991,
Pubmed
Dabiri,
Listeria monocytogenes moves rapidly through the host-cell cytoplasm by inducing directional actin assembly.
1990,
Pubmed
Dabora,
The microtubule-dependent formation of a tubulovesicular network with characteristics of the ER from cultured cell extracts.
1988,
Pubmed
Dailey,
Dynamics of the endoplasmic reticulum and other membranous organelles in growth cones of cultured neurons.
1989,
Pubmed
Dailey,
Structure and organization of membrane organelles along distal microtubule segments in growth cones.
1991,
Pubmed
Desai,
A new role for motor proteins as couplers to depolymerizing microtubules.
1995,
Pubmed
Drechsel,
The minimum GTP cap required to stabilize microtubules.
1994,
Pubmed
Dye,
Taxol-induced flexibility of microtubules and its reversal by MAP-2 and Tau.
1993,
Pubmed
Erickson,
Microtubule dynamic instability and GTP hydrolysis.
1992,
Pubmed
Gard,
Microtubule assembly in cytoplasmic extracts of Xenopus oocytes and eggs.
1987,
Pubmed
,
Xenbase
Gittes,
Flexural rigidity of microtubules and actin filaments measured from thermal fluctuations in shape.
1993,
Pubmed
Görlich,
A mammalian homolog of SEC61p and SECYp is associated with ribosomes and nascent polypeptides during translocation.
1992,
Pubmed
Hill,
Theoretical problems related to the attachment of microtubules to kinetochores.
1985,
Pubmed
Hyman,
Structural changes accompanying GTP hydrolysis in microtubules: information from a slowly hydrolyzable analogue guanylyl-(alpha,beta)-methylene-diphosphonate.
1995,
Pubmed
Koshland,
Polewards chromosome movement driven by microtubule depolymerization in vitro.
1988,
Pubmed
Lee,
Construction of the endoplasmic reticulum.
1989,
Pubmed
Lombillo,
Minus-end-directed motion of kinesin-coated microspheres driven by microtubule depolymerization.
1995,
Pubmed
Lombillo,
Antibodies to the kinesin motor domain and CENP-E inhibit microtubule depolymerization-dependent motion of chromosomes in vitro.
1995,
Pubmed
Lutz,
Micromanipulation studies of the asymmetric positioning of the maturation spindle in Chaetopterus sp. oocytes: I. Anchorage of the spindle to the cortex and migration of a displaced spindle.
1988,
Pubmed
Mitchison,
Polewards microtubule flux in the mitotic spindle: evidence from photoactivation of fluorescence.
1989,
Pubmed
Mitchison,
Poleward kinetochore fiber movement occurs during both metaphase and anaphase-A in newt lung cell mitosis.
1992,
Pubmed
Miyata,
Morphological changes in liposomes caused by polymerization of encapsulated actin and spontaneous formation of actin bundles.
1992,
Pubmed
Munro,
An Hsp70-like protein in the ER: identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein.
1986,
Pubmed
Murray,
Cell cycle extracts.
1991,
Pubmed
Peskin,
Cellular motions and thermal fluctuations: the Brownian ratchet.
1993,
Pubmed
Pierre,
CLIP-170 links endocytic vesicles to microtubules.
1992,
Pubmed
Rieder,
Motile kinetochores and polar ejection forces dictate chromosome position on the vertebrate mitotic spindle.
1994,
Pubmed
Rosenfeld,
Biosynthesis and processing of ribophorins in the endoplasmic reticulum.
1984,
Pubmed
Salmon,
High resolution multimode digital imaging system for mitosis studies in vivo and in vitro.
1994,
Pubmed
,
Xenbase
Sammak,
Direct observation of microtubule dynamics in living cells.
1988,
Pubmed
Sanger,
Host cell actin assembly is necessary and likely to provide the propulsive force for intracellular movement of Listeria monocytogenes.
1992,
Pubmed
Sawin,
Poleward microtubule flux mitotic spindles assembled in vitro.
1991,
Pubmed
,
Xenbase
Scheel,
Motor protein independent binding of endocytic carrier vesicles to microtubules in vitro.
1991,
Pubmed
Shelden,
Observation and quantification of individual microtubule behavior in vivo: microtubule dynamics are cell-type specific.
1993,
Pubmed
Skibbens,
Directional instability of kinetochore motility during chromosome congression and segregation in mitotic newt lung cells: a push-pull mechanism.
1993,
Pubmed
Terasaki,
Localization of endoplasmic reticulum in living and glutaraldehyde-fixed cells with fluorescent dyes.
1984,
Pubmed
Terasaki,
Microtubules and the endoplasmic reticulum are highly interdependent structures.
1986,
Pubmed
Terasaki,
Interactions among endoplasmic reticulum, microtubules, and retrograde movements of the cell surface.
1994,
Pubmed
,
Xenbase
Theriot,
Actin microfilament dynamics in locomoting cells.
1991,
Pubmed
Theriot,
The rate of actin-based motility of intracellular Listeria monocytogenes equals the rate of actin polymerization.
1992,
Pubmed
Tilney,
Acrosomal reaction of Thyone sperm. II. The kinetics and possible mechanism of acrosomal process elongation.
1982,
Pubmed
Vale,
Formation of membrane networks in vitro by kinesin-driven microtubule movement.
1988,
Pubmed
Vale,
One-dimensional diffusion of microtubules bound to flagellar dynein.
1989,
Pubmed
Venier,
Analysis of microtubule rigidity using hydrodynamic flow and thermal fluctuations.
1994,
Pubmed
Verde,
Control of microtubule dynamics and length by cyclin A- and cyclin B-dependent kinases in Xenopus egg extracts.
1992,
Pubmed
,
Xenbase
Walker,
Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies.
1988,
Pubmed
Waters,
The force-producing mechanism for centrosome separation during spindle formation in vertebrates is intrinsic to each aster.
1993,
Pubmed
Yeh,
Spindle dynamics and cell cycle regulation of dynein in the budding yeast, Saccharomyces cerevisiae.
1995,
Pubmed