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Effects of vasopressin and aldosterone on the lateral mobility of epithelial Na+ channels in A6 renal epithelial cells.
Smith PR
,
Stoner LC
,
Viggiano SC
,
Angelides KJ
,
Benos DJ
.
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We have previously demonstrated that apical Na+ channels in A6 renal epithelial cells are associated with spectrin-based membrane cytoskeleton proteins and that the lateral mobility of these channels, as determined by fluorescence photobleach recovery (FPR) analysis, is severely restricted by this association (Smith et al., 1991. Proc. Natl. Acad. Sci. USA 88:6971-6975). Recent data indicate that the actin component of the cytoskeleton may play a role in modulating Na+ channel activity (Cantiello et al., 1991. Am. J. Physiol. 261:C882-C888); however, it is unknown if the Na+ channel's linkage to the spectrin-based membrane cytoskeleton is also involved in regulating channel activity. In this study, we have used FPR to examine if the linkage of the Na+ channels to the membrane cytoskeleton is a site for modulation of Na+ channel activity in filter grown A6 cells by vasopressin and aldosterone. We hypothesized that if the linkage of the Na+ channels to the membrane cytoskeleton is a site for regulation of Na+ channel activity by vasopressin and aldosterone, then hormone-mediated changes in either the membrane cytoskeleton or the affinity of the Na+ channel for the membrane cytoskeleton, should be reflected in changes in the lateral mobility and/or mobile fraction of Na+ channels on the cell surface. FPR revealed that although the rates of lateral mobility were not affected, there was a twofold increase in mobility fraction (f) of apical Na+ channels in aldosterone-treated (16 hr) monolayers (f = 32.31 +/- 5.42%) when compared to control (unstimulated) (f = 14.2 +/- 0.77%) and vasopressin-treated (20 min) (f = 12.7 +/- 2.4%) monolayers. The twofold increase in mobile fraction of Na+ channels corresponds to the average increase in Na+ transport in response to aldosterone in A6 cells. The aldosterone-induced increase in Na+ transport and mobile fraction can be inhibited by the methylation inhibitor, 3-deazaadenosine, consistent with the hypothesis that a methylation event is involved in aldosterone induced upregulation of Na+ transport. We propose that the membrane cytoskeleton is involved in the aldosterone-mediated activation of epithelial Na+ channels.
Angelides,
Distribution and lateral mobility of voltage-dependent sodium channels in neurons.
1988, Pubmed
Angelides,
Distribution and lateral mobility of voltage-dependent sodium channels in neurons.
1988,
Pubmed
Axelrod,
Mobility measurement by analysis of fluorescence photobleaching recovery kinetics.
1976,
Pubmed
Bennett,
The spectrin-based membrane skeleton and micron-scale organization of the plasma membrane.
1993,
Pubmed
Benos,
Purification and characterization of the amiloride-sensitive sodium channel from A6 cultured cells and bovine renal papilla.
1986,
Pubmed
Benos,
Molecular characteristics of amiloride-sensitive sodium channels.
1992,
Pubmed
Benos,
The epithelial sodium channel. Subunit number and location of the amiloride binding site.
1987,
Pubmed
Cantiello,
Actin filaments regulate epithelial Na+ channel activity.
1991,
Pubmed
Cantiello,
G protein subunit, alpha i-3, activates a pertussis toxin-sensitive Na+ channel from the epithelial cell line, A6.
1989,
Pubmed
Cantiello,
G alpha i-3 regulates epithelial Na+ channels by activation of phospholipase A2 and lipoxygenase pathways.
1990,
Pubmed
,
Xenbase
Chang,
Lateral mobility of erythrocyte membrane proteins studied by the fluorescence photobleaching recovery technique.
1981,
Pubmed
Ding,
Vasopressin depolymerizes F-actin in toad bladder epithelial cells.
1991,
Pubmed
Dragsten,
Membrane asymmetry in epithelia: is the tight junction a barrier to diffusion in the plasma membrane?
1981,
Pubmed
Edidin,
Differences between the lateral organization of conventional and inositol phospholipid-anchored membrane proteins. A further definition of micrometer scale membrane domains.
1991,
Pubmed
Franki,
Effect of cytochalasin D on the actin cytoskeleton of the toad bladder epithelial cell.
1992,
Pubmed
Garty,
Mechanisms of aldosterone action in tight epithelia.
1986,
Pubmed
Golan,
Lateral mobility of band 3 in the human erythrocyte membrane studied by fluorescence photobleaching recovery: evidence for control by cytoskeletal interactions.
1980,
Pubmed
Goodman,
Aldosterone-Induced Membrane Phospholipid Fatty Acid Metabolism in the Toad Urinary Bladder.
1975,
Pubmed
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Hamilton,
Single-channel recordings from two types of amiloride-sensitive epithelial Na+ channels.
1986,
Pubmed
Ismailov,
Protein kinase A phosphorylation and G protein regulation of purified renal Na+ channels in planar bilayer membranes.
1994,
Pubmed
Ismailov,
Carboxyl methylation activates purified renal amiloride-sensitive Na+ channels in planar lipid bilayers.
1994,
Pubmed
Johnson,
A cytoskeletal mechanism for Ca2+ channel metabolic dependence and inactivation by intracellular Ca2+.
1993,
Pubmed
Kemendy,
Aldosterone alters the open probability of amiloride-blockable sodium channels in A6 epithelia.
1992,
Pubmed
Kleyman,
Aldosterone does not alter apical cell-surface expression of epithelial Na+ channels in the amphibian cell line A6.
1992,
Pubmed
,
Xenbase
Kleyman,
Arginine vasopressin and forskolin regulate apical cell surface expression of epithelial Na+ channels in A6 cells.
1994,
Pubmed
,
Xenbase
Kusumi,
Confined lateral diffusion of membrane receptors as studied by single particle tracking (nanovid microscopy). Effects of calcium-induced differentiation in cultured epithelial cells.
1993,
Pubmed
Lien,
Effects of an acetyl-coenzyme A carboxylase inhibitor and a sodium-sparing diuretic on aldosterone-stimulated sodium transport, lipid synthesis, and phospholipid fatty acid composition in the toad urinary bladder.
1975,
Pubmed
Madreperla,
Na+,K+-adenosine triphosphatase polarity in retinal photoreceptors: a role for cytoskeletal attachments.
1989,
Pubmed
Oh,
Regulation by phosphorylation of purified epithelial Na+ channels in planar lipid bilayers.
1993,
Pubmed
Ohara,
G protein activation inhibits amiloride-blockable highly selective sodium channels in A6 cells.
1993,
Pubmed
,
Xenbase
Paller,
Lateral mobility of Na,K-ATPase and membrane lipids in renal cells. Importance of cytoskeletal integrity.
1994,
Pubmed
Palmer,
Epithelial Na channels: function and diversity.
1992,
Pubmed
Prat,
Vasopressin and protein kinase A activate G protein-sensitive epithelial Na+ channels.
1993,
Pubmed
Prat,
Activation of epithelial Na+ channels by protein kinase A requires actin filaments.
1993,
Pubmed
Rosenmund,
Calcium-induced actin depolymerization reduces NMDA channel activity.
1993,
Pubmed
Sariban-Sohraby,
Aldosterone-induced and GTP-stimulated methylation of a 90-kDa polypeptide in the apical membrane of A6 epithelia.
1993,
Pubmed
,
Xenbase
Sariban-Sohraby,
Detergent solubilization, functional reconstitution, and partial purification of epithelial amiloride-binding protein.
1986,
Pubmed
Sariban-Sohraby,
Methylation increases sodium transport into A6 apical membrane vesicles: possible mode of aldosterone action.
1984,
Pubmed
Schwiebert,
Actin-based cytoskeleton regulates a chloride channel and cell volume in a renal cortical collecting duct cell line.
1994,
Pubmed
Simon,
Vasopressin depolymerizes apical F-actin in rat inner medullary collecting duct.
1993,
Pubmed
Smith,
Amiloride-sensitive sodium channel is linked to the cytoskeleton in renal epithelial cells.
1991,
Pubmed
,
Xenbase
Sorscher,
Antibodies against purified epithelial sodium channel protein from bovine renal papilla.
1988,
Pubmed
Suzuki,
F-actin network may regulate a Cl- channel in renal proximal tubule cells.
1993,
Pubmed
Tsuji,
Restriction of the lateral motion of band 3 in the erythrocyte membrane by the cytoskeletal network: dependence on spectrin association state.
1986,
Pubmed
Wang,
Involvement of actin cytoskeleton in modulation of apical K channel activity in rat collecting duct.
1994,
Pubmed
Wiesmann,
Aldosterone-stimulated transmethylations are linked to sodium transport.
1985,
Pubmed
Wills,
Effects of aldosterone on the impedance properties of cultured renal amphibian epithelia.
1993,
Pubmed
,
Xenbase
Zhang,
Protein lateral mobility as a reflection of membrane microstructure.
1993,
Pubmed