Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Pflugers Arch
1995 Nov 01;4311:32-45. doi: 10.1007/bf00374375.
Show Gene links
Show Anatomy links
Basolateral membrane chloride permeability of A6 cells: implication in cell volume regulation.
Brochiero E
,
Banderali U
,
Lindenthal S
,
Raschi C
,
Ehrenfeld J
.
???displayArticle.abstract???
The permeability to Cl- of the basolateral membrane (blm) was investigated in renal (A6) epithelial cells, assessing their role in transepithelial ion transport under steady-state conditions (isoosmotic) and following a hypoosmotic shock (i.e. in a regulatory volume decrease, RVD). Three different complementary studies were made by measuring: (1) the Cl- transport rates (delta F/Fo s-1 (x10(-3))), where F is the fluorescence of N-(6-methoxyquinoyl) acetoethyl ester, MQAE, and Fo the maximal fluorescence (x10(-3)) of both membranes by following the intracellular Cl- activities (ai Cl-, measured with MQAE) after extracellular Cl- substitution (2) the blm 86Rb and 36Cl uptakes and (3) the cellular potential and Cl- current using the whole-cell patch-clamp technique to differentiate between the different Cl- transport mechanisms. The permeability of the blm to Cl- was found to be much greater than that of the apical membranes under resting conditions: aiCl- changes were 5.3 +/- 0.7 mM and 25.5 +/- 1.05 mM (n = 79) when Cl- was substituted by NO3(-) in the media bathing apical and basolateral membranes. The Cl- transport rate of the blm was blocked by bumetanide (100 microM) and 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB, 50 microM) but not by N-phenylanthranilic acid (DPC, 100 microM). 86Rb and 36Cl uptake experiments confirmed the presence of a bumetanide- and a NPPB-sensitive Cl- pathway, the latter being approximately three times more important than the former (Na/K/2Cl cotransporter). Appli-cation of a hypoosmotic medium to the serosal side of the cell increased delta F/Fo s-1 (x10(-3)) after extracellular Cl- substitution (1.03 +/- 0.10 and 2.45 +/- 0.17 arbitrary fluorescent units s-1 for isoosmotic and hypoosmotic conditions respectively, n = 11); this delta F/Fo s-1 (x10(-3)) increase was totally blocked by serosal NPPB application; on the other hand, cotransporter activity was decreased by the hypoosmotic shock. Cellular Ca2+ depletion had no effect on delta F/Fo s-1 (x10(-3)) under isoosmotic conditions, but blocked the delta F/Fo s-1 (x10(-3)) increase induced by a hypoosmotic stress. Under isotonic conditions the measured cellular potential at rest was -37.2 +/- 4.0 mV but reached a maximal and transient depolarization of -25.1 +/- 3.7 mV (n = 9) under hypoosmotic conditions. The cellular current at a patch-clamping cellular potential of -85 mV (close to the Nernst equilibrium potential for K+) was blocked by NPPB and transiently increased by hypoosmotic shock (≈50% maximum increase). This study demonstrates that the major component of Cl- transport through the blm of the A6 monolayer is a conductive pathway (NPPB-sensitive Cl- channels) and not a Na/K/2Cl cotransporter. These channels could play a role in transepithelial Cl- absorption and cell volume regulation. The increase in the blm Cl- conductance, inducing a depolarization of these membranes, is proposed as one of the early events responsible for the stimulation of the 86Rb efflux involved in cell volume regulation.
Ackerman,
Hypotonicity activates a native chloride current in Xenopus oocytes.
1994, Pubmed,
Xenbase
Ackerman,
Hypotonicity activates a native chloride current in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Broillet,
Basolateral membrane potassium conductance of A6 cells.
1991,
Pubmed
,
Xenbase
Brown,
Characterization of a Na : K : 2C1 cotransport system in the apical membrane of a renal epithelial cell line (LLC-PK1).
1985,
Pubmed
Chalfant,
Distinct regulation of Na+ reabsorption and Cl- secretion by arginine vasopressin in the amphibian cell line A6.
1993,
Pubmed
,
Xenbase
Chao,
Chloride conductive and cotransport mechanisms in cultures of canine tracheal epithelial cells measured by an entrapped fluorescent indicator.
1990,
Pubmed
Crowe,
Apical membrane sodium and chloride entry during osmotic swelling of renal (A6) epithelial cells.
1995,
Pubmed
Dubé,
Hypotonic shock activates a maxi K+ channel in primary cultured proximal tubule cells.
1990,
Pubmed
Ehrenfeld,
Basolateral potassium membrane permeability of A6 cells and cell volume regulation.
1994,
Pubmed
,
Xenbase
Fan,
Identification of a regulated Na/K/Cl cotransport system in a distal nephron cell line.
1992,
Pubmed
,
Xenbase
Geck,
Na+ + K+ + 2Cl- cotransport in animal cells--its role in volume regulation.
1985,
Pubmed
Gill,
Separation of drug transport and chloride channel functions of the human multidrug resistance P-glycoprotein.
1992,
Pubmed
Granitzer,
Osmotic swelling and membrane conductances in A6 cells.
1992,
Pubmed
,
Xenbase
Granitzer,
Basolateral membrane conductance in A6 cells: effect of high sodium transport rate.
1992,
Pubmed
Gründer,
Regions involved in the opening of CIC-2 chloride channel by voltage and cell volume.
,
Pubmed
,
Xenbase
Handler,
Toad urinary bladder epithelial cells in culture: maintenance of epithelial structure, sodium transport, and response to hormones.
1979,
Pubmed
Horn,
Muscarinic activation of ionic currents measured by a new whole-cell recording method.
1988,
Pubmed
Keeler,
Evidence that prostaglandin E2 stimulates chloride secretion in cultured A6 renal epithelial cells.
1986,
Pubmed
Kotera,
Calcium-dependent chloride current activated by hyposmotic stress in rat lacrimal acinar cells.
1993,
Pubmed
Krapivinsky,
Molecular characterization of a swelling-induced chloride conductance regulatory protein, pICln.
1994,
Pubmed
,
Xenbase
Kregenow,
Osmoregulatory salt transporting mechanisms: control of cell volume in anisotonic media.
1981,
Pubmed
Kubo,
Volume-regulatory Cl- channel currents in cultured human epithelial cells.
1992,
Pubmed
Kunzelmann,
Small-conductance Cl- channels in HT29 cells: activation by Ca2+, hypotonic cell swelling and 8-Br-cGMP.
1992,
Pubmed
Lau,
Intracellular Cl- concentration in striated intralobular ducts from rabbit mandibular salivary glands.
1994,
Pubmed
Marunaka,
Effects of insulin and phosphatase on a Ca2(+)-dependent Cl- channel in a distal nephron cell line (A6).
1990,
Pubmed
Marunaka,
Modification of Ca(2+)-sensitivity of Ca(2+)-activated Cl- channel by vasopressin and cholera toxin.
1993,
Pubmed
Marunaka,
Effects of vasopressin on single Cl- channels in the apical membrane of distal nephron cells (A6).
1993,
Pubmed
Marunaka,
Chloride channels in the apical membrane of a distal nephron A6 cell line.
1990,
Pubmed
McCann,
Identification and regulation of whole-cell chloride currents in airway epithelium.
1989,
Pubmed
Middleton,
Nucleotide receptors regulate membrane ion transport in renal epithelial cells.
1993,
Pubmed
Nagel,
The intracellular electrical potential profile of the frog skin epithelium.
1976,
Pubmed
Nilius,
Volume-activated Cl- currents in different mammalian non-excitable cell types.
1994,
Pubmed
O'Neill,
Regulation of vascular endothelial cell volume by Na-K-2Cl cotransport.
1992,
Pubmed
Paulmichl,
The effect of hypoosmolarity on the electrical properties of Madin Darby canine kidney cells.
1989,
Pubmed
Paulmichl,
New mammalian chloride channel identified by expression cloning.
1992,
Pubmed
,
Xenbase
Perkins,
Transport properties of toad kidney epithelia in culture.
1981,
Pubmed
,
Xenbase
Poncet,
Chloride channels in apical membrane of primary cultures of rabbit distal bright convoluted tubule.
1994,
Pubmed
Rindler,
(Na+,K+)-cotransport in the Madin-Darby canine kidney cell line. Kinetic characterization of the interaction between Na+ and K+.
1982,
Pubmed
Rothstein,
Volume-activated K+ and Cl- pathways of dissociated epithelial cells (MDCK): role of Ca2+.
1990,
Pubmed
Roy,
Effect of anisotonic media on volume, ion and amino-acid content and membrane potential of kidney cells (MDCK) in culture.
1987,
Pubmed
Sariban-Sohraby,
Apical sodium uptake in toad kidney epithelial cell line A6.
1983,
Pubmed
Solc,
Swelling-induced and depolarization-induced C1-channels in normal and cystic fibrosis epithelial cells.
1991,
Pubmed
Ubl,
Simultaneous recording of cell volume, membrane current and membrane potential: effect of hypotonic shock.
1989,
Pubmed
Ussing,
Volume regulation and basolateral co-transport of sodium, potassium, and chloride ions in frog skin epithelium.
1985,
Pubmed
Valverde,
Volume-regulated chloride channels associated with the human multidrug-resistance P-glycoprotein.
1992,
Pubmed
Van Driessche,
An automatic monitoring system for epithelial cell height.
1993,
Pubmed
Verkman,
Development and biological applications of chloride-sensitive fluorescent indicators.
1990,
Pubmed
Verrey,
Regulation by aldosterone of Na+,K+-ATPase mRNAs, protein synthesis, and sodium transport in cultured kidney cells.
1987,
Pubmed
,
Xenbase
Verrey,
Antidiuretic hormone action in A6 cells: effect on apical Cl and Na conductances and synergism with aldosterone for NaCl reabsorption.
1994,
Pubmed
,
Xenbase
Wills,
Amiloride-sensitive Na+ transport across cultured renal (A6) epithelium: evidence for large currents and high Na:K selectivity.
1990,
Pubmed
,
Xenbase
Willumsen,
Activation of an apical Cl- conductance by Ca2+ ionophores in cystic fibrosis airway epithelia.
1989,
Pubmed
Worrell,
A volume-sensitive chloride conductance in human colonic cell line T84.
1989,
Pubmed
Yanase,
Adenosine 3',5'-cyclic monophosphate stimulates chloride secretion in A6 epithelia.
1986,
Pubmed
,
Xenbase