Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1995 Dec 01;1512:6663-9. doi: 10.1128/MCB.15.12.6663.
Show Gene links
Show Anatomy links
Modular structure of chromosomal proteins HMG-14 and HMG-17: definition of a transcriptional enhancement domain distinct from the nucleosomal binding domain.
Trieschmann L
,
Postnikov YV
,
Rickers A
,
Bustin M
.
???displayArticle.abstract???
Chromosomal proteins HMG-14 and HMG-17 are the only known nuclear proteins which specifically bind to the nucleosome core particle and are implicated in the generation and/or maintenance of structural features specific to active chromatin. The two proteins facilitate polymerase II and III transcription from in vitro- and in vivo-assembled circular chromatin templates. Here we used deletion mutants and specific peptides to identify the transcriptional enhancement domain and delineate the nucleosomal binding domain of the HMG-14 and -17 proteins. Deletion of the 22 C-terminal amino acids of HMG-17 or 26 C-terminal amino acids of HMG-14 reduces significantly the ability of the proteins to enhance transcription from chromatin templates. In contrast, N-terminal truncation mutants had the same transcriptional enhancement activity as the full-length proteins. We conclude that the negatively charged C-terminal region of the proteins is required for transcriptional enhancement. Chromatin transcription enhancement assays, which involve binding competition between the full-length proteins and peptides derived from their nucleosomal binding regions, indicate that the minimal nucleosomal binding domain of human HMG-17 is 24 amino acids long and spans residues 17 to 40. The results suggest that HMG-14 and -17 proteins have a modular structure and contain distinct functional domains.
Abercrombie,
Studies on the conformational properties of the high-mobility-group chromosomal protein HMG 17 and its interaction with DNA.
1978, Pubmed
Abercrombie,
Studies on the conformational properties of the high-mobility-group chromosomal protein HMG 17 and its interaction with DNA.
1978,
Pubmed
Albright,
Subunit structures of different electrophoretic forms of nucleosomes.
1980,
Pubmed
Alfonso,
The footprint of chromosomal proteins HMG-14 and HMG-17 on chromatin subunits.
1994,
Pubmed
Allan,
Participation of core histone "tails" in the stabilization of the chromatin solenoid.
1982,
Pubmed
Almouzni,
Assembly of spaced chromatin promoted by DNA synthesis in extracts from Xenopus eggs.
1988,
Pubmed
,
Xenbase
Almouzni,
Chromatin assembly on replicating DNA in vitro.
1990,
Pubmed
,
Xenbase
Blair,
Mutational analysis of the transcription activation domain of RelA: identification of a highly synergistic minimal acidic activation module.
1994,
Pubmed
Brawley,
HMG proteins 14 and 17 become cross-linked to the globular domain of histone H3 near the nucleosome core particle dyad.
1992,
Pubmed
Bustin,
Structural features of the HMG chromosomal proteins and their genes.
1990,
Pubmed
Bustin,
Immunochemical analysis of the exposure of high mobility group protein 14 and 17 surfaces in chromatin.
1990,
Pubmed
Bustin,
Recombinant human chromosomal proteins HMG-14 and HMG-17.
1991,
Pubmed
Cary,
Structural studies on two high-mobility-group proteins from calf thymus, HMG-14 and HMG-20 (ubiquitin), and their interaction with DNA.
1980,
Pubmed
Cook,
High mobility group protein 17 cross-links primarily to histone H2A in the reconstituted HMG 17-nucleosome core particle complex.
1986,
Pubmed
Cook,
Analysis of the binding of high mobility group protein 17 to the nucleosome core particle by 1H NMR spectroscopy.
1989,
Pubmed
Crippa,
Nucleosome core binding region of chromosomal protein HMG-17 acts as an independent functional domain.
1992,
Pubmed
Crippa,
Deposition of chromosomal protein HMG-17 during replication affects the nucleosomal ladder and transcriptional potential of nascent chromatin.
1993,
Pubmed
,
Xenbase
Ding,
Stimulation of RNA polymerase II elongation by chromosomal protein HMG-14.
1994,
Pubmed
Dorbic,
Chromatin from transcribed genes contains HMG17 only downstream from the starting point of transcription.
1987,
Pubmed
Druckmann,
Immunofractionation of DNA sequences associated with HMG-17 in chromatin.
1986,
Pubmed
Einck,
Inhibition of transcription in somatic cells by microinjection of antibodies to chromosomal proteins.
1983,
Pubmed
Garcia-Ramirez,
Role of the histone "tails" in the folding of oligonucleosomes depleted of histone H1.
1992,
Pubmed
Hahn,
Structure(?) and function of acidic transcription activators.
1993,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Landsman,
Chromosomal protein HMG-17. Complete human cDNA sequence and evidence for a multigene family.
1986,
Pubmed
Landsman,
Chromosomal protein HMG-14. Complete human cDNA sequence and evidence for a multigene family.
1986,
Pubmed
Landsman,
Assessment of the transcriptional activation potential of the HMG chromosomal proteins.
1991,
Pubmed
Li,
Acidic transcription factors alleviate nucleosome-mediated repression of DNA replication of bovine papillomavirus type 1.
1994,
Pubmed
Malik,
Enrichment of acetylated histones in polynucleosomes containing high mobility group protein 17 revealed by immunoaffinity chromatography.
1984,
Pubmed
Mardian,
Nucleosome cores have two specific binding sites for nonhistone chromosomal proteins HMG 14 and HMG 17.
1980,
Pubmed
Paranjape,
Role of chromatin structure in the regulation of transcription by RNA polymerase II.
1994,
Pubmed
Peterson,
Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA.
1980,
Pubmed
,
Xenbase
Postnikov,
Distribution of high mobility group proteins 1/2, E and 14/17 and linker histones H1 and H5 on transcribed and non-transcribed regions of chicken erythrocyte chromatin.
1991,
Pubmed
Postnikov,
The cooperative binding of chromosomal protein HMG-14 to nucleosome cores is reduced by single point mutations in the nucleosomal binding domain.
1994,
Pubmed
Roberts,
Activator-induced conformational change in general transcription factor TFIIB.
1994,
Pubmed
Sandeen,
The interaction of high mobility proteins HMG14 and 17 with nucleosomes.
1980,
Pubmed
Svaren,
The transactivation domain of Pho4 is required for nucleosome disruption at the PHO5 promoter.
1994,
Pubmed
Tjian,
Transcriptional activation: a complex puzzle with few easy pieces.
1994,
Pubmed
Trieschmann,
Modular structure of chromosomal proteins HMG-14 and HMG-17: definition of a transcriptional enhancement domain distinct from the nucleosomal binding domain.
1995,
Pubmed
,
Xenbase
Trieschmann,
Incorporation of chromosomal proteins HMG-14/HMG-17 into nascent nucleosomes induces an extended chromatin conformation and enhances the utilization of active transcription complexes.
1995,
Pubmed
,
Xenbase
