Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-18886
Biochem Biophys Res Commun 1995 Dec 14;2172:419-27.
Show Gene links Show Anatomy links

Distinct phosphorylation sites differentially influence facilitated transport of an NLS-protein and its subsequent intranuclear binding.

Vancurova I , Jochova-Rupes J , Lou W , Paine PL .


???displayArticle.abstract???
We measured the nuclear transport of radiolabeled fusion proteins consisting of variants of the Simian Virus 40 large T antigen's nuclear localization sequence region linked to beta-galactosidase, itself a cytoplasmic protein. We microinjected the fusion protein variants into the cytoplasm of living Xenopus oocytes or supplied them to the surface of oil-isolated oocyte nuclei via paired beads or cytoplasm. Presence of the cdc2 kinase site (124T) on the amino flank of the nuclear localization sequence (126PKKKRKV132) greatly enhances facilitated transport through the nuclear pore complex; additional presence of the casein kinase II site (112S) enhances subsequent intranuclear binding.

???displayArticle.pubmedLink??? 7503717
???displayArticle.link??? Biochem Biophys Res Commun
???displayArticle.grants??? [+]

Species referenced: Xenopus laevis
Genes referenced: cdk1