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Characterization of an insulin from the three-toed amphiuma (Amphibia: Urodela) with an N-terminally extended A-chain and high receptor-binding affinity.
Conlon JM
,
Cavanaugh ES
,
Mynarcik DC
,
Whittaker J
.
???displayArticle.abstract??? Insulin was isolated from an extract of the pancreas of a urodele, the three-toed amphiuma (Amphiuma tridactylum), and its primary structure established as Ala-Arg-Gly-Ile-Val-Glu-Gln-Cys-Cys-His10-Asn-Thr-Cys- Ser-Leu-Asn-Gln-Leu-Glu-Asn20-Tyr-Cys-Asn for the A-chain and Ile-Thr-Asn-Gln-Tyr-Leu-Cys-Gly-Ser-His10-Leu-Val-Glu-Ala- Leu-Tyr-Leu-Val-Cys-Gly20-Asp-Arg-Gly-Phe-Phe-Tyr-Ser-Pro-Lys for the B-chain. The N-terminus of the A-chain is extended by two amino acids (Ala-Arg) relative to all other known insulins suggesting an anomalous pathway of post-translational processing in the region of the C-peptide/A-chain junction of proinsulin. In common with chicken and Xenopus insulins, which contain a HisA8, amphiuma insulin was more potent (approx. 5-fold) than porcine insulin in inhibiting the binding of [125I-TyrA14]insulin to the soluble human insulin receptor from transfected 293EBNA cells (an adenovirus-transformed human kidney cell line). This result is consistent with previous data showing that insulin analogues extended at GlyA1 by uncharged groups have reduced binding affinity whereas high affinity is preserved in analogues extended by basic amino acid residues.
Andersen,
Neuropeptides in the amphibian brain.
1992, Pubmed,
Xenbase
Andersen,
Neuropeptides in the amphibian brain.
1992,
Pubmed
,
Xenbase
Bailey,
Role of ovarian hormones in the long-term control of glucose homeostasis. Effects of insulin secretion.
1980,
Pubmed
Bajaj,
Evolution in the insulin family: molecular clocks that tell the wrong time.
1984,
Pubmed
Baker,
The structure of 2Zn pig insulin crystals at 1.5 A resolution.
1988,
Pubmed
Chan,
Messenger RNA sequence and primary structure of preproinsulin in a primitive vertebrate, the Atlantic hagfish.
1981,
Pubmed
Conlon,
Isolation and structural characterization of insulin, glucagon and somatostatin from the turtle, Pseudemys scripta.
1990,
Pubmed
Conlon,
Characterization of insulin, glucagon, and somatostatin from the river lamprey, Lampetra fluviatilis.
1995,
Pubmed
Conlon,
Multiple molecular forms of insulin and glucagon-like peptide from the Pacific ratfish (Hydrolagus colliei).
1989,
Pubmed
Gammeltoft,
Insulin receptors: binding kinetics and structure-function relationship of insulin.
1984,
Pubmed
Gliemann,
The biological activity and the binding affinity of modified insulins determined on isolated rat fat cells.
1974,
Pubmed
Hedges,
Tetrapod phylogeny inferred from 18S and 28S ribosomal RNA sequences and a review of the evidence for amniote relationships.
1990,
Pubmed
Katsoyannis,
Analogs of insulin. 1. Synthesis of destripeptide B 28-30 bovine insulin and destripeptide B 28-30 porcine (human) insulin.
1971,
Pubmed
Lipkind,
Molecular modeling of the substrate specificity of prohormone convertases SPC2 and SPC3.
1995,
Pubmed
Lomedico,
The structure and evolution of the two nonallelic rat preproinsulin genes.
1979,
Pubmed
Markussen,
Soluble, prolonged-acting insulin derivatives. I. Degree of protraction and crystallizability of insulins substituted in the termini of the B-chain.
1987,
Pubmed
Perler,
The evolution of genes: the chicken preproinsulin gene.
1980,
Pubmed
Plisetskaya,
Characterization of coho salmon (Oncorhynchus kisutch) insulin.
1985,
Pubmed
Plisetskaya,
Isolation and structure of lamprey (Petromyzon marinus) insulin.
1988,
Pubmed
Rösen,
Binding of insulin to bovine liver plasma membrane. Use of insulin analogues modified at the A1 residue.
1980,
Pubmed
Schäffer,
A model for insulin binding to the insulin receptor.
1994,
Pubmed
Shuldiner,
Xenopus laevis contains two nonallelic preproinsulin genes. cDNA cloning and evolutionary perspective.
1989,
Pubmed
,
Xenbase
Shuldiner,
Isolation and characterization of two different insulins from an amphibian, Xenopus laevis.
1989,
Pubmed
,
Xenbase
Simon,
Enhanced binding affinity of chicken insulin in rat liver membranes and human lymphocytes: relationship tothe kinetic properties of the hormone- receptor interaction.
1977,
Pubmed
Smeekens,
Proinsulin processing by the subtilisin-related proprotein convertases furin, PC2, and PC3.
1992,
Pubmed
Steiner,
The new enzymology of precursor processing endoproteases.
1992,
Pubmed
Waelbroeck,
The pH dependence of insulin binding. A quantitative study.
1982,
Pubmed
Williams,
Mapping of an NH2-terminal ligand binding site of the insulin receptor by alanine scanning mutagenesis.
1995,
Pubmed
