Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Pflugers Arch
1996 Jan 01;4313:408-14. doi: 10.1007/bf02207279.
Show Gene links
Show Anatomy links
Glycerol permeability of mutant aquaporin 1 and other AQP-MIP proteins: inhibition studies.
Abrami L
,
Berthonaud V
,
Deen PM
,
Rousselet G
,
Tacnet F
,
Ripoche P
.
???displayArticle.abstract???
In a recent work, we showed that the aquaporins 1 (AQP1) are permeable to certain small solutes such as glycerol. Here, we have further investigated the permeation pathway of glycerol through human AQP1 (hAQP1) by the use of mutants (C189S, H180A, H209A) and inhibitors such as P-chloromercuribenzene sulphonate (pCMBS), CuSO4 or phloretin, in comparison with other AQP-MIP (where MIP denotes major intrinsic protein) proteins: hAQP2, plant water channel gammaTIP and bacterial glycerol permease facilitator, GlpF. Glycerol movements were measured in Xenopus laevis oocytes. Apparent glycerol permeability coefficients (P'gly) were calculated from the rates of oocyte swelling upon exposure to an isoosmotic medium containing an inwardly directed gradient of glycerol and from [3H]glycerol uptake measurements. Similar P'gly values were obtained for hAQP1 and hAQP2 6 to 8 times greater than control indicating that hAQP2 also transports glycerol. P'gly of hAQP2-injected oocytes was pCMBS and CuSO4 sensitive. In contrast, the P'gly value of gammaTIP was close to that of control, indicating that gammaTIP does not transport glycerol. The hAQP1-C189S, -H180A and -H209A mutants gave P'gly values similar to those obtained for wild hAQP1, indicating that these mutations did not affect glycerol movements. However, the H209A mutant has an osmotic water permeability coefficient (Pf) value decreased by 50%. The inhibitory effect pCMBS on P'gly was maintained for the 2 His mutants and, more interestingly, was also conserved for the C189S mutant. CuSO4 significantly inhibited P'gly of oocytes expressing hAQP1, hAQP1-C189S, -H180A, and -H209A mutants and had no effect on P'gly of GlpF-injected oocytes. Phloretin was shown to inhibit by around 80% the glycerol fluxes of wild and mutant hAQP1, hAQP2 and to fully inhibit glycerol uptake in GlpF-injected oocytes.
Abrami,
Sequence and functional expression of an amphibian water channel, FA-CHIP: a new member of the MIP family.
1994, Pubmed,
Xenbase
Abrami,
Sequence and functional expression of an amphibian water channel, FA-CHIP: a new member of the MIP family.
1994,
Pubmed
,
Xenbase
Abrami,
Evidence for a glycerol pathway through aquaporin 1 (CHIP28) channels.
1995,
Pubmed
,
Xenbase
Agre,
Aquaporin CHIP: the archetypal molecular water channel.
1993,
Pubmed
,
Xenbase
Carlsen,
Glycerol transport in human red cells.
1976,
Pubmed
Deen,
Requirement of human renal water channel aquaporin-2 for vasopressin-dependent concentration of urine.
1994,
Pubmed
,
Xenbase
Denker,
Identification, purification, and partial characterization of a novel Mr 28,000 integral membrane protein from erythrocytes and renal tubules.
1988,
Pubmed
Echevarria,
Cloning and expression of AQP3, a water channel from the medullary collecting duct of rat kidney.
1994,
Pubmed
,
Xenbase
Fushimi,
Cloning and expression of apical membrane water channel of rat kidney collecting tubule.
1993,
Pubmed
,
Xenbase
Hasegawa,
Molecular cloning of a mercurial-insensitive water channel expressed in selected water-transporting tissues.
1994,
Pubmed
,
Xenbase
Heller,
Substrate specificity and transport properties of the glycerol facilitator of Escherichia coli.
1980,
Pubmed
Ishibashi,
Molecular cloning and expression of a member of the aquaporin family with permeability to glycerol and urea in addition to water expressed at the basolateral membrane of kidney collecting duct cells.
1994,
Pubmed
,
Xenbase
Iyer,
Synthesis of the native copper(II)-transport site of human serum albumin and its copper(II)-binding properties.
1978,
Pubmed
Jung,
Molecular structure of the water channel through aquaporin CHIP. The hourglass model.
1994,
Pubmed
,
Xenbase
Jung,
Molecular characterization of an aquaporin cDNA from brain: candidate osmoreceptor and regulator of water balance.
1994,
Pubmed
,
Xenbase
Ma,
Cloning of a water channel homolog expressed in brain meningeal cells and kidney collecting duct that functions as a stilbene-sensitive glycerol transporter.
1994,
Pubmed
,
Xenbase
Maurel,
Functional characterization of the Escherichia coli glycerol facilitator, GlpF, in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Maurel,
The vacuolar membrane protein gamma-TIP creates water specific channels in Xenopus oocytes.
1993,
Pubmed
,
Xenbase
Mühlrad,
Studies on the properties of chemically modified actin. 3. Carbethoxylation.
1969,
Pubmed
Nielsen,
Vasopressin increases water permeability of kidney collecting duct by inducing translocation of aquaporin-CD water channels to plasma membrane.
1995,
Pubmed
Preston,
Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein.
1992,
Pubmed
,
Xenbase
Preston,
The mercury-sensitive residue at cysteine 189 in the CHIP28 water channel.
1993,
Pubmed
,
Xenbase
Preston,
Isolation of the cDNA for erythrocyte integral membrane protein of 28 kilodaltons: member of an ancient channel family.
1991,
Pubmed
Verkman,
Water permeability and fluidity of renal basolateral membranes.
1986,
Pubmed
Zeidel,
Reconstitution of functional water channels in liposomes containing purified red cell CHIP28 protein.
1992,
Pubmed
,
Xenbase
Zeidel,
Ultrastructure, pharmacologic inhibition, and transport selectivity of aquaporin channel-forming integral protein in proteoliposomes.
1994,
Pubmed
Zhang,
A point mutation at cysteine 189 blocks the water permeability of rat kidney water channel CHIP28k.
1993,
Pubmed
,
Xenbase
Zhang,
Cloning, functional analysis and cell localization of a kidney proximal tubule water transporter homologous to CHIP28.
1993,
Pubmed
,
Xenbase