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Clin Exp Metastasis
1996 Mar 01;142:107-14. doi: 10.1007/bf00121207.
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CD44 exon variant 6 epitope and hyaluronate synthase are expressed on HT29 human colorectal carcinoma cells in a SCID mouse model of metastasis formation.
Mitchell BS
,
Whitehouse A
,
Prehm P
,
Delpech B
,
Schumacher U
.
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The factors which lead to the formation of metastases are generally poorly understood; however the expression of a particular variant of the cell adhesion molecule CD44 may be important in facilitating metastasis formation in colon cancer. The aim of the present study was to investigate the expression of CD44 exon v 6 (CD44v6), hyaluronate (one of its ligands), and hyaluronate synthase, in a clinically relevant animal model of metastatic colon carcinoma. HT29 human colon carcinoma cells were injected subcutaneously between the scapulae of severe combined immunodeficient (SCID) mice and left for 3 weeks (by which time the tumours had produced metastases in the lungs). Morphological observations at the tumour-host interface were consistent with the dissociation of neoplastic cells from the primary tumours, and the ability of these cells to migrate through the extracellular matrix facilitating metastasis formation. Immunohistochemically detectable hyaluronate synthase expression was increased in vivo compared with the parent cell line in vitro. CD44v6 expression and hyaluronate were increased around single cells at the periphery of tumours compared with the central regions. CD44v6 and hyaluronate snythase expression were co-expressed in the same cells. Indeed, the present study is the first to demonstrate hyaluronate synthase expression by an epithelial cell type.
Aruffo,
CD44 is the principal cell surface receptor for hyaluronate.
1990, Pubmed
Aruffo,
CD44 is the principal cell surface receptor for hyaluronate.
1990,
Pubmed
Bartolazzi,
Interaction between CD44 and hyaluronate is directly implicated in the regulation of tumor development.
1994,
Pubmed
Brecht,
Increased hyaluronate synthesis is required for fibroblast detachment and mitosis.
1986,
Pubmed
,
Xenbase
Buehring,
Human breast tumor cell lines: identity evaluation by ultrastructure.
1974,
Pubmed
Bumpers,
Human villous adenomas engrafted into scid mice survive for prolonged period without malignant transformation.
1994,
Pubmed
Carter,
Characterization of the class III collagen receptor, a phosphorylated, transmembrane glycoprotein expressed in nucleated human cells.
1988,
Pubmed
DeAngelis,
Molecular cloning, identification, and sequence of the hyaluronan synthase gene from group A Streptococcus pyogenes.
1993,
Pubmed
,
Xenbase
Finke,
Colorectal cancer prognosis and expression of exon-v6-containing CD44 proteins.
1995,
Pubmed
Hart,
Molecular aspects of the metastatic cascade.
1989,
Pubmed
Hashimoto,
Collagenolytic activities of squamous cell carcinoma of the skin.
1973,
Pubmed
Hashimoto,
Electron microscopic observations of collagenolytic activity of basal cell epithelioma of the skin in vivo and in vitro.
1972,
Pubmed
Heider,
A human homologue of the rat metastasis-associated variant of CD44 is expressed in colorectal carcinomas and adenomatous polyps.
1993,
Pubmed
Hudson,
CD44 is the major peanut lectin-binding glycoprotein of human epidermal keratinocytes and plays a role in intercellular adhesion.
1995,
Pubmed
Jalkanen,
Human lymphocyte and lymphoma homing receptors.
1987,
Pubmed
Klewes,
The hyaluronate synthase from a eukaryotic cell line.
1993,
Pubmed
,
Xenbase
Knudson,
Epithelial-mesenchymal interaction in the regulation of hyaluronate production during limb development.
1988,
Pubmed
Knudson,
Stimulation of glycosaminoglycan production in murine tumors.
1984,
Pubmed
Koretz,
Effect of CD44v6 on survival in colorectal carcinoma.
1995,
Pubmed
LUIBEL,
An electron microscopic study of carcinoma in situ and invasive carcinoma of the cervix uteri.
1960,
Pubmed
Lansing,
Hyaluronate synthase: cloning and sequencing of the gene from Streptococcus sp.
1993,
Pubmed
,
Xenbase
Lévesque,
Localization and solubilization of hyaluronan and of the hyaluronan-binding protein hyaluronectin in human normal and arteriosclerotic arterial walls.
1994,
Pubmed
Matsumura,
Significance of CD44 gene products for cancer diagnosis and disease evaluation.
1992,
Pubmed
Mulder,
Colorectal cancer prognosis and expression of exon-v6-containing CD44 proteins.
1994,
Pubmed
Prehm,
Hyaluronate is synthesized at plasma membranes.
1984,
Pubmed
Prehm,
Synthesis of hyaluronate in differentiated teratocarcinoma cells. Characterization of the synthase.
1983,
Pubmed
,
Xenbase
Rooney,
The role of hyaluronan in tumour neovascularization (review).
1995,
Pubmed
Schumacher,
Helix pomatia agglutinin binding is a useful prognostic indicator in colorectal carcinoma.
1994,
Pubmed
Schumacher,
Glycosylation patterns of the human colon cancer cell line HT-29 detected by Helix pomatia agglutinin and other lectins in culture, in primary tumours and in metastases in SCID mice.
1994,
Pubmed
Sleeman,
The role of CD44 splice variants in human metastatic cancer.
1995,
Pubmed
Sofroniew,
Long-term storage and regular repeated use of diluted antisera in glass staining jars for increased sensitivity, reproducibility, and convenience of single- and two-color light microscopic immunocytochemistry.
1982,
Pubmed
Stamenkovic,
The hematopoietic and epithelial forms of CD44 are distinct polypeptides with different adhesion potentials for hyaluronate-bearing cells.
1991,
Pubmed
Terpe,
CD44 variant isoforms are preferentially expressed in basal epithelial of non-malignant human fetal and adult tissues.
1994,
Pubmed
,
Xenbase
Toole,
Hyaluronate and invasiveness of the rabbit V2 carcinoma.
1979,
Pubmed
Turley,
Characterization of hyaluronate binding proteins isolated from 3T3 and murine sarcoma virus transformed 3T3 cells.
1987,
Pubmed
Turley,
Localization of hyaluronate and hyaluronate-binding protein on motile and non-motile fibroblasts.
1985,
Pubmed
