Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1996 May 28;9311:5443-8. doi: 10.1073/pnas.93.11.5443.
Show Gene links
Show Anatomy links
Conserved motifs in prokaryotic and eukaryotic polypeptide release factors: tRNA-protein mimicry hypothesis.
Ito K
,
Ebihara K
,
Uno M
,
Nakamura Y
.
???displayArticle.abstract???
Translation termination requires two codon-specific polypeptide release factors in prokaryotes and one omnipotent factor in eukaryotes. Sequences of 17 different polypeptide release factors from prokaryotes and eukaryotes were compared. The prokaryotic release factors share residues split into seven motifs. Conservation of many discrete, perhaps critical, amino acids is observed in eukaryotic release factors, as well as in the C-terminal portion of elongation factor (EF) G. Given that the C-terminal domains of EF-G interacts with ribosomes by mimicry of a tRNA structure, the pattern of conservation of residues in release factors may reflect requirements for a tRNA-mimicry for binding to the A site of the ribosome. This mimicry would explain why release factors recognize stop codons and suggests that all prokaryotic and eukaryotic release factors evolved from the progenitor of EF-G.
AEvarsson,
Three-dimensional structure of the ribosomal translocase: elongation factor G from Thermus thermophilus.
1994, Pubmed
AEvarsson,
Three-dimensional structure of the ribosomal translocase: elongation factor G from Thermus thermophilus.
1994,
Pubmed
Breining,
Yeast omnipotent supressor SUP1 (SUP45): nucleotide sequence of the wildtype and a mutant gene.
1986,
Pubmed
Capecchi,
Characterization of three proteins involved in polypeptide chain termination.
1969,
Pubmed
Caskey,
Peptide chain termination, codon, protein factor, and ribosomal requirements.
1969,
Pubmed
Craigen,
Bacterial peptide chain release factors: conserved primary structure and possible frameshift regulation of release factor 2.
1985,
Pubmed
Craigen,
Recent advances in peptide chain termination.
1990,
Pubmed
Czworkowski,
The crystal structure of elongation factor G complexed with GDP, at 2.7 A resolution.
1994,
Pubmed
Devereux,
A comprehensive set of sequence analysis programs for the VAX.
1984,
Pubmed
Doel,
The dominant PNM2- mutation which eliminates the psi factor of Saccharomyces cerevisiae is the result of a missense mutation in the SUP35 gene.
1994,
Pubmed
Doudna,
Selection of an RNA molecule that mimics a major autoantigenic epitope of human insulin receptor.
1995,
Pubmed
Elliott,
Cloning, genetic characterization, and nucleotide sequence of the hemA-prfA operon of Salmonella typhimurium.
1989,
Pubmed
Elliott,
Salmonella typhimurium prfA mutants defective in release factor 1.
1991,
Pubmed
Fleischmann,
Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.
1995,
Pubmed
Fraser,
The minimal gene complement of Mycoplasma genitalium.
1995,
Pubmed
Frolova,
A highly conserved eukaryotic protein family possessing properties of polypeptide chain release factor.
1994,
Pubmed
,
Xenbase
Goldstein,
Peptide chain termination: effect of protein S on ribosomal binding of release factors.
1970,
Pubmed
Grentzmann,
Localization and characterization of the gene encoding release factor RF3 in Escherichia coli.
1994,
Pubmed
Kawakami,
Chromosomal location and structure of the operon encoding peptide-chain-release factor 2 of Escherichia coli.
1988,
Pubmed
Kawakami,
Autogenous suppression of an opal mutation in the gene encoding peptide chain release factor 2.
1990,
Pubmed
Kawazu,
Comparative characterization of release factor RF-3 genes of Escherichia coli, Salmonella typhimurium, and Dichelobacter nodosus.
1995,
Pubmed
Konecki,
Characterization of reticulocyte release factor.
1977,
Pubmed
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1985,
Pubmed
Mikuni,
Sequence and functional analysis of mutations in the gene encoding peptide-chain-release factor 2 of Escherichia coli.
1991,
Pubmed
Mikuni,
Identification of the prfC gene, which encodes peptide-chain-release factor 3 of Escherichia coli.
1994,
Pubmed
Mironova,
Reversions to respiratory competence of omnipotent sup45 suppressor mutants may be caused by secondary sup45 mutations.
1995,
Pubmed
Moffat,
Interaction of the release factors with the Escherichia coli ribosome: structurally and functionally-important domains.
1991,
Pubmed
Mol,
Crystal structure of human uracil-DNA glycosylase in complex with a protein inhibitor: protein mimicry of DNA.
1995,
Pubmed
Nissen,
Crystal structure of the ternary complex of Phe-tRNAPhe, EF-Tu, and a GTP analog.
1995,
Pubmed
Ogawara,
Properties of peptide chain release factor 2 from Streptomyces coelicolor A3(2): conserved primary structure but no frameshift regulation.
1995,
Pubmed
Pel,
Sequence comparison of new prokaryotic and mitochondrial members of the polypeptide chain release factor family predicts a five-domain model for release factor structure.
1992,
Pubmed
Pel,
Single point mutations in domain II of the yeast mitochondrial release factor mRF-1 affect ribosome binding.
1993,
Pubmed
Scolnick,
Release factors differing in specificity for terminator codons.
1968,
Pubmed
Sikorski,
A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
1989,
Pubmed
Stansfield,
Ribosomal association of the yeast SAL4 (SUP45) gene product: implications for its role in translation fidelity and termination.
1992,
Pubmed
Stansfield,
Polypeptide chain termination in Saccharomyces cerevisiae.
1994,
Pubmed
Stansfield,
The products of the SUP45 (eRF1) and SUP35 genes interact to mediate translation termination in Saccharomyces cerevisiae.
1995,
Pubmed
,
Xenbase
Tate,
Translational termination: "stop" for protein synthesis or "pause" for regulation of gene expression.
1992,
Pubmed
Ter-Avanesyan,
The SUP35 omnipotent suppressor gene is involved in the maintenance of the non-Mendelian determinant [psi+] in the yeast Saccharomyces cerevisiae.
1994,
Pubmed
Weiss,
Genetic screen for cloned release factor genes.
1984,
Pubmed
Zhang,
Genetic implication for an interaction between release factor one and ribosomal protein L7/L12 in vivo.
1994,
Pubmed
Zhouravleva,
Termination of translation in eukaryotes is governed by two interacting polypeptide chain release factors, eRF1 and eRF3.
1995,
Pubmed
,
Xenbase